Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing
Abstract Wound dressing development is an area of active research. Traditional dressings lack antibacterial activity, biocompatibility, and tissue regeneration. Alginate is a heavily investigated polymer employed as wound dressings and can be combined with a wide range of additives. Herein, we repor...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06836-5 |
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| author | Suhair Hikmat Ola Tarawneh Lama Hamadneh Rania Hamed Ala A. Alhusban Mohammad Hailat Hadeel Abu Mahfouz Sawsan Shraim Alghadeer Al-Shammari Aya Aljariri Rafa Abu Rayya Lana Hamdan |
| author_facet | Suhair Hikmat Ola Tarawneh Lama Hamadneh Rania Hamed Ala A. Alhusban Mohammad Hailat Hadeel Abu Mahfouz Sawsan Shraim Alghadeer Al-Shammari Aya Aljariri Rafa Abu Rayya Lana Hamdan |
| author_sort | Suhair Hikmat |
| collection | DOAJ |
| description | Abstract Wound dressing development is an area of active research. Traditional dressings lack antibacterial activity, biocompatibility, and tissue regeneration. Alginate is a heavily investigated polymer employed as wound dressings and can be combined with a wide range of additives. Herein, we report the preparation of alginate gel using the crosslinking technique as potential wound dressing, with insight investigation of the influence of employing single, two, or three cross-linkers: Strontium (Sr), zinc oxide (ZnO), and gentamicin sulfate. Rheology was used to confirm the gel’s preparation, where the samples’ viscosity curves show decreased viscosity with increased shear rate, indicating pseudoplastic flow. The linear viscoelastic region shows constant G’ and G” within the sample structure. In this study, we used three gels with different mixtures of ingredients: Gels A, B, and C contain sodium alginate (1% w/v) and 0.5 mL of Sr nitrate (4% w/v). However, Gels B and C contain 0.25 mL of ZnO (0.5% w/v). Gel C also includes 0.1 mL of gentamicin (1% w/v). The study examined the effectiveness of Gel A, B, and C on wound healing, calculating the reduction of wound area after seven, 14, and 20 days of a single topical treatment. Gel A, B, and C significantly reduced wound area, while Gel B and C showed a significant reduction. The zone of inhibition was used to detect the gels’ efficacy against microorganisms. The study found zinc deposition in the liver and bone, with Gel B and C showing higher levels. The study also found significant overexpression of MIP α and MIP β in tissues and downregulation of CCL2, IL8, and TGF β, explaining wound healing with minimal scar formation. Graphical Abstract |
| format | Article |
| id | doaj-art-e120e3c4ab3f458fb1e095b9498c5782 |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-e120e3c4ab3f458fb1e095b9498c57822025-01-12T12:06:18ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382025-01-0136111110.1007/s10856-024-06836-5Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healingSuhair Hikmat0Ola Tarawneh1Lama Hamadneh2Rania Hamed3Ala A. Alhusban4Mohammad Hailat5Hadeel Abu Mahfouz6Sawsan Shraim7Alghadeer Al-Shammari8Aya Aljariri9Rafa Abu Rayya10Lana Hamdan11Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Basic Medical Sciences, Faculty of Medicine, Al-Balqa Applied UniversityDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanDepartment of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of JordanAbstract Wound dressing development is an area of active research. Traditional dressings lack antibacterial activity, biocompatibility, and tissue regeneration. Alginate is a heavily investigated polymer employed as wound dressings and can be combined with a wide range of additives. Herein, we report the preparation of alginate gel using the crosslinking technique as potential wound dressing, with insight investigation of the influence of employing single, two, or three cross-linkers: Strontium (Sr), zinc oxide (ZnO), and gentamicin sulfate. Rheology was used to confirm the gel’s preparation, where the samples’ viscosity curves show decreased viscosity with increased shear rate, indicating pseudoplastic flow. The linear viscoelastic region shows constant G’ and G” within the sample structure. In this study, we used three gels with different mixtures of ingredients: Gels A, B, and C contain sodium alginate (1% w/v) and 0.5 mL of Sr nitrate (4% w/v). However, Gels B and C contain 0.25 mL of ZnO (0.5% w/v). Gel C also includes 0.1 mL of gentamicin (1% w/v). The study examined the effectiveness of Gel A, B, and C on wound healing, calculating the reduction of wound area after seven, 14, and 20 days of a single topical treatment. Gel A, B, and C significantly reduced wound area, while Gel B and C showed a significant reduction. The zone of inhibition was used to detect the gels’ efficacy against microorganisms. The study found zinc deposition in the liver and bone, with Gel B and C showing higher levels. The study also found significant overexpression of MIP α and MIP β in tissues and downregulation of CCL2, IL8, and TGF β, explaining wound healing with minimal scar formation. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06836-5 |
| spellingShingle | Suhair Hikmat Ola Tarawneh Lama Hamadneh Rania Hamed Ala A. Alhusban Mohammad Hailat Hadeel Abu Mahfouz Sawsan Shraim Alghadeer Al-Shammari Aya Aljariri Rafa Abu Rayya Lana Hamdan Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing Journal of Materials Science: Materials in Medicine |
| title | Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing |
| title_full | Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing |
| title_fullStr | Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing |
| title_full_unstemmed | Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing |
| title_short | Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing |
| title_sort | strontium nitrate dopped zinc oxide loaded alginate gels with gentamicin for improved wound healing |
| url | https://doi.org/10.1007/s10856-024-06836-5 |
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