Fast and Sensitive Detection of Anti-SARS-CoV-2 IgG Using SiO<sub>2</sub>@Au@CDs Nanoparticle-Based Lateral Flow Immunoassay Strip Coupled with Miniaturized Fluorimeter
The development of a novel strategy for the measurement of SARS-CoV-2 IgG antibodies is of vital significance for <i>COVID-19</i> diagnosis and effect of vaccination evaluation. In this investigation, an SiO<sub>2</sub>@Au@CDs nanoparticle (NP)-based lateral flow immunoassay...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/14/12/1568 |
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| Summary: | The development of a novel strategy for the measurement of SARS-CoV-2 IgG antibodies is of vital significance for <i>COVID-19</i> diagnosis and effect of vaccination evaluation. In this investigation, an SiO<sub>2</sub>@Au@CDs nanoparticle (NP)-based lateral flow immunoassay (LFIA) strip was fabricated and coupled with a miniaturized fluorimeter. The morphology features and particle sizes of the SiO<sub>2</sub>@Au@CDs NPs were characterized carefully, and the results indicated that the materials possess monodisperse, uniform, and spherical structures. Finally, this system was employed for SARS-CoV-2 IgG antibody test. In this work, the strategy for the SARS-CoV-2 IgG antibody test possesses several merits, such as speed (less than 15 min), high sensitivity (1.2 × 10<sup>−7</sup> mg/mL), broad linearity range (7.4 × 10<sup>−7</sup>~7.4 × 10<sup>−4</sup> mg/mL), accurate results, high selectivity, good stability, and low cost. Additionally, future trends in LFAs using quantum dot-based diagnostics are envisioned. |
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| ISSN: | 2218-273X |