OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression
Plants are susceptible to infection by various pathogens with high epidemic potential. Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight in rice, one of the most significant diseases in both temperate and tropical regions. In this study, we report the identification and characterization of...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1519039/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841553926691749888 |
|---|---|
| author | Win Tun Kieu Thi Xuan Vo Behnam Derakhshani Jinmi Yoon Lae-Hyeon Cho Kay Tha Ye Soe Win Sang-Won Lee Ki-Hong Jung Jong-Seong Jeon Gynheung An |
| author_facet | Win Tun Kieu Thi Xuan Vo Behnam Derakhshani Jinmi Yoon Lae-Hyeon Cho Kay Tha Ye Soe Win Sang-Won Lee Ki-Hong Jung Jong-Seong Jeon Gynheung An |
| author_sort | Win Tun |
| collection | DOAJ |
| description | Plants are susceptible to infection by various pathogens with high epidemic potential. Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight in rice, one of the most significant diseases in both temperate and tropical regions. In this study, we report the identification and characterization of OsWRKY26, a sucrose-inducible transcription factor, that plays a role in the plant defense responses following Xoo infection. We found that mutant plants with defective OsWRKY26 showed enhanced defense response specifically to Xoo, indicating that this transcription factor acts as a negative defense regulator. In contrast, mutant plants did not exhibit higher resistance compared to wild-type (WT) plants when infected with the rice blast fungal pathogen Magnaporthe oryzae. Transcriptomic analysis of mutant and WT plants revealed that several pathogen resistance genes were upregulated in mutants. Of these, we selected OsXa39 for further analysis. Transient expression experiments in rice protoplasts showed that OsWRKY26 repressed the expression of a Luciferase reporter gene driven by the OsXa39 promoter. Chromatin immunoprecipitation analysis revealed that OsWRKY26 binds directly to the promoter region of OsXa39. These findings suggest that OsWRKY26 negatively regulates the defense response during Xoo infection by repressing OsXa39 as well as other pathogen-related genes such as OsXa47, OsBBR1, OsRSR1, OsPR1a, OsPR1-11, OsPR2, and OsPR4c. |
| format | Article |
| id | doaj-art-e0b45e992a824c64adbaaeeb9a710fcb |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-e0b45e992a824c64adbaaeeb9a710fcb2025-01-09T06:10:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15190391519039OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expressionWin Tun0Kieu Thi Xuan Vo1Behnam Derakhshani2Jinmi Yoon3Lae-Hyeon Cho4Kay Tha Ye Soe Win5Sang-Won Lee6Ki-Hong Jung7Jong-Seong Jeon8Gynheung An9Graduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaDepartment of Biological Sciences and Bioengineering, Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University, Incheon, Republic of KoreaDepartment of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of KoreaPlants are susceptible to infection by various pathogens with high epidemic potential. Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight in rice, one of the most significant diseases in both temperate and tropical regions. In this study, we report the identification and characterization of OsWRKY26, a sucrose-inducible transcription factor, that plays a role in the plant defense responses following Xoo infection. We found that mutant plants with defective OsWRKY26 showed enhanced defense response specifically to Xoo, indicating that this transcription factor acts as a negative defense regulator. In contrast, mutant plants did not exhibit higher resistance compared to wild-type (WT) plants when infected with the rice blast fungal pathogen Magnaporthe oryzae. Transcriptomic analysis of mutant and WT plants revealed that several pathogen resistance genes were upregulated in mutants. Of these, we selected OsXa39 for further analysis. Transient expression experiments in rice protoplasts showed that OsWRKY26 repressed the expression of a Luciferase reporter gene driven by the OsXa39 promoter. Chromatin immunoprecipitation analysis revealed that OsWRKY26 binds directly to the promoter region of OsXa39. These findings suggest that OsWRKY26 negatively regulates the defense response during Xoo infection by repressing OsXa39 as well as other pathogen-related genes such as OsXa47, OsBBR1, OsRSR1, OsPR1a, OsPR1-11, OsPR2, and OsPR4c.https://www.frontiersin.org/articles/10.3389/fpls.2024.1519039/fulldiseaseOsWRKY26OsXa39riceXanthomonas oryzae |
| spellingShingle | Win Tun Kieu Thi Xuan Vo Behnam Derakhshani Jinmi Yoon Lae-Hyeon Cho Kay Tha Ye Soe Win Sang-Won Lee Ki-Hong Jung Jong-Seong Jeon Gynheung An OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression Frontiers in Plant Science disease OsWRKY26 OsXa39 rice Xanthomonas oryzae |
| title | OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression |
| title_full | OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression |
| title_fullStr | OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression |
| title_full_unstemmed | OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression |
| title_short | OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression |
| title_sort | oswrky26 negatively regulates bacterial blight resistance by suppressing osxa39 expression |
| topic | disease OsWRKY26 OsXa39 rice Xanthomonas oryzae |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1519039/full |
| work_keys_str_mv | AT wintun oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT kieuthixuanvo oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT behnamderakhshani oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT jinmiyoon oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT laehyeoncho oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT kaythayesoewin oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT sangwonlee oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT kihongjung oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT jongseongjeon oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression AT gynheungan oswrky26negativelyregulatesbacterialblightresistancebysuppressingosxa39expression |