Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice
In recent years, various gene therapy strategies have been developed for cancer treatment. One of these strategies is electroporation-based delivery of therapeutic transgenes - gene electrotransfer (GET). Electrochemotherapy and GET have been combined in several contemporary preclinical and veterina...
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| Format: | Article |
| Language: | English |
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Sciendo
2025-04-01
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| Series: | Radiology and Oncology |
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| Online Access: | https://doi.org/10.2478/raon-2025-0027 |
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| author | Omerzel Masa Markelc Bostjan Brezar Simona Kranjc Sersa Gregor Cemazar Maja |
| author_facet | Omerzel Masa Markelc Bostjan Brezar Simona Kranjc Sersa Gregor Cemazar Maja |
| author_sort | Omerzel Masa |
| collection | DOAJ |
| description | In recent years, various gene therapy strategies have been developed for cancer treatment. One of these strategies is electroporation-based delivery of therapeutic transgenes - gene electrotransfer (GET). Electrochemotherapy and GET have been combined in several contemporary preclinical and veterinary studies. In most cases, two different pulse protocols are used, each for a specific treatment. The aim of our current study was to test whether the standard pulse protocol used in daily clinical practice for electrochemotherapy can also be used for effective GET. |
| format | Article |
| id | doaj-art-e09a72a362e74534ac230c3fe3fd596a |
| institution | Kabale University |
| issn | 1581-3207 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Radiology and Oncology |
| spelling | doaj-art-e09a72a362e74534ac230c3fe3fd596a2025-08-20T04:03:13ZengSciendoRadiology and Oncology1581-32072025-04-0159220321210.2478/raon-2025-0027Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in miceOmerzel Masa0Markelc Bostjan1Brezar Simona Kranjc2Sersa Gregor3Cemazar Maja41Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia1Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia1Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia1Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia1Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, SloveniaIn recent years, various gene therapy strategies have been developed for cancer treatment. One of these strategies is electroporation-based delivery of therapeutic transgenes - gene electrotransfer (GET). Electrochemotherapy and GET have been combined in several contemporary preclinical and veterinary studies. In most cases, two different pulse protocols are used, each for a specific treatment. The aim of our current study was to test whether the standard pulse protocol used in daily clinical practice for electrochemotherapy can also be used for effective GET.https://doi.org/10.2478/raon-2025-0027gene electrotransferelectrochemotherapycellstumormuscle |
| spellingShingle | Omerzel Masa Markelc Bostjan Brezar Simona Kranjc Sersa Gregor Cemazar Maja Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice Radiology and Oncology gene electrotransfer electrochemotherapy cells tumor muscle |
| title | Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice |
| title_full | Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice |
| title_fullStr | Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice |
| title_full_unstemmed | Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice |
| title_short | Efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice |
| title_sort | efficient gene transfer by pulse parameters for electrochemotherapy of cells in vitro and in muscle and melanoma tumors in mice |
| topic | gene electrotransfer electrochemotherapy cells tumor muscle |
| url | https://doi.org/10.2478/raon-2025-0027 |
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