Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression
Tea plant can enrich a large amount of fluorine (F) in the cell wall of its mature leaves, thus posing the risk of excessive intake of F for tea consumers. This study investigated the effect of foliar calcium (Ca) application (0.05-1 mM) on F accumulation in tea plant leaves by analyzing the associa...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1443439/full |
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| author | Jinlei Luo Jinlei Luo Lintao Zhang Daili Li Shuangjie Huang Chunlei Li Yi Chen Yi Chen Peng Yin Wei Liu Mufang Sun Mufang Sun Guiyi Guo Guiyi Guo Guiyi Guo |
| author_facet | Jinlei Luo Jinlei Luo Lintao Zhang Daili Li Shuangjie Huang Chunlei Li Yi Chen Yi Chen Peng Yin Wei Liu Mufang Sun Mufang Sun Guiyi Guo Guiyi Guo Guiyi Guo |
| author_sort | Jinlei Luo |
| collection | DOAJ |
| description | Tea plant can enrich a large amount of fluorine (F) in the cell wall of its mature leaves, thus posing the risk of excessive intake of F for tea consumers. This study investigated the effect of foliar calcium (Ca) application (0.05-1 mM) on F accumulation in tea plant leaves by analyzing the association of F with cell wall materials, pectin methylesterification structure, and cell wall genes. Ca spray could effectively reduce the F level, the content of wall carbohydrates (excluding the chelate pectin fraction) and the pectin methylesterification degree in tea plant leaves. Correlation analysis further revealed that the tea leaf F content was positively correlated with most of the cell wall materials. Transcriptomics analysis indicated that the key genes involved in reorganization of cell wall polysaccharides (such as CslE6, XTH32, PG, PGIP, PME18, PMEU1, and PMEI9) were associated with the variations in F content and cell wall components. All the results suggest that Ca may lessen the tea leaf F level by reducing the accumulation of cell wall materials and intervening the cell wall structure and gene expression. This study provided useful information for reducing the F level and solving the F safety problem in tea plant. |
| format | Article |
| id | doaj-art-e09a5900084c46e6b8e3c04cf2a85e7c |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-e09a5900084c46e6b8e3c04cf2a85e7c2025-01-03T06:47:05ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14434391443439Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expressionJinlei Luo0Jinlei Luo1Lintao Zhang2Daili Li3Shuangjie Huang4Chunlei Li5Yi Chen6Yi Chen7Peng Yin8Wei Liu9Mufang Sun10Mufang Sun11Guiyi Guo12Guiyi Guo13Guiyi Guo14Xinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaDabie Mountain Laboratory, Xinyang, Henan, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaShandong Provincial University Laboratory for Protected Horticulture, Weifang University of Science and Technology, Shouguang, Shandong, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaDabie Mountain Laboratory, Xinyang, Henan, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaDabie Mountain Laboratory, Xinyang, Henan, ChinaXinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, Xinyang, Henan, ChinaDabie Mountain Laboratory, Xinyang, Henan, ChinaXinyang Wenxin Tea Co., Ltd., Xinyang, Henan, ChinaTea plant can enrich a large amount of fluorine (F) in the cell wall of its mature leaves, thus posing the risk of excessive intake of F for tea consumers. This study investigated the effect of foliar calcium (Ca) application (0.05-1 mM) on F accumulation in tea plant leaves by analyzing the association of F with cell wall materials, pectin methylesterification structure, and cell wall genes. Ca spray could effectively reduce the F level, the content of wall carbohydrates (excluding the chelate pectin fraction) and the pectin methylesterification degree in tea plant leaves. Correlation analysis further revealed that the tea leaf F content was positively correlated with most of the cell wall materials. Transcriptomics analysis indicated that the key genes involved in reorganization of cell wall polysaccharides (such as CslE6, XTH32, PG, PGIP, PME18, PMEU1, and PMEI9) were associated with the variations in F content and cell wall components. All the results suggest that Ca may lessen the tea leaf F level by reducing the accumulation of cell wall materials and intervening the cell wall structure and gene expression. This study provided useful information for reducing the F level and solving the F safety problem in tea plant.https://www.frontiersin.org/articles/10.3389/fpls.2024.1443439/fulltea plantfluorinecalciumcell walltranscriptomics |
| spellingShingle | Jinlei Luo Jinlei Luo Lintao Zhang Daili Li Shuangjie Huang Chunlei Li Yi Chen Yi Chen Peng Yin Wei Liu Mufang Sun Mufang Sun Guiyi Guo Guiyi Guo Guiyi Guo Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression Frontiers in Plant Science tea plant fluorine calcium cell wall transcriptomics |
| title | Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression |
| title_full | Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression |
| title_fullStr | Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression |
| title_full_unstemmed | Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression |
| title_short | Foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression |
| title_sort | foliar calcium application reduces fluorine accumulation in tea plant by regulating cell wall structure and gene expression |
| topic | tea plant fluorine calcium cell wall transcriptomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1443439/full |
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