Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model
Developing sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with 18F-labeling tracers for assessing liver fibrosis in a rat model with...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-01-01
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| Series: | Molecular Imaging |
| Online Access: | http://dx.doi.org/10.1155/2021/7545284 |
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| author | Chun-Yi Wu Hsin-Hua Hsieh Pei-An Chu Wen-Hsiang Hong Ting-Yu Chang Chia-Fang Hsu Siao-Ting Lin Po-Hsun Su Shin-Lei Peng |
| author_facet | Chun-Yi Wu Hsin-Hua Hsieh Pei-An Chu Wen-Hsiang Hong Ting-Yu Chang Chia-Fang Hsu Siao-Ting Lin Po-Hsun Su Shin-Lei Peng |
| author_sort | Chun-Yi Wu |
| collection | DOAJ |
| description | Developing sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with 18F-labeling tracers for assessing liver fibrosis in a rat model with bile duct ligation (BDL). Twenty-one 6-week-old Sprague-Dawley male rats were used in this study. Longitudinal PET images using [18F]N-2-(2-fluoroethoxy)benzyl)-N-(4-phenoxypyridin-3-yl)acetamide ([18F]FEPPA) (n=3), [18F]fluoroacetate ([18F]FAc) (n=3), and 18F-fluoro-2-deoxy-D-glucose ([18F]FDG) (n=3) were obtained at 0, 1, and 2 weeks after BDL. Biochemical assays, histological assays, immunohistochemical staining assays, and next generation sequencing analyses were also performed at 0 (n=3), 1 (n=3), 2 (n=3), and 3 (n=3) weeks after BDL, which demonstrated the severe damage in rat livers after BDL. Regarding [18F]FEPPA and [18F]FDG, there was a significantly higher uptake in the liver after BDL (both P<0.05), which lasted until week 2. However, the uptake of [18F]FAc in the liver was not significantly different before and after BDL (P=0.28). Collectively, both [18F]FEPPA and [18F]FDG can serve as sensitive probes for detecting the liver fibrosis. However, [18F]FAc is not recommended to diagnose liver fibrosis. |
| format | Article |
| id | doaj-art-e0948ff8ee7f4e6f88ff3365e9a15973 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-e0948ff8ee7f4e6f88ff3365e9a159732025-01-03T00:11:12ZengSAGE PublishingMolecular Imaging1536-01212021-01-01202110.1155/2021/7545284Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat ModelChun-Yi Wu0Hsin-Hua Hsieh1Pei-An Chu2Wen-Hsiang Hong3Ting-Yu Chang4Chia-Fang Hsu5Siao-Ting Lin6Po-Hsun Su7Shin-Lei Peng8Department of Biomedical Imaging and Radiological SciencesDepartment of Biomedical Imaging and Radiological SciencesDepartment of Biomedical Imaging and Radiological ScienceDepartment of Biomedical Imaging and Radiological SciencesDepartment of Biomedical Imaging and Radiological SciencesDepartment of Biomedical Imaging and Radiological ScienceDepartment of Biomedical Imaging and Radiological ScienceDepartment of Biomedical Imaging and Radiological ScienceDepartment of Biomedical Imaging and Radiological ScienceDeveloping sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with 18F-labeling tracers for assessing liver fibrosis in a rat model with bile duct ligation (BDL). Twenty-one 6-week-old Sprague-Dawley male rats were used in this study. Longitudinal PET images using [18F]N-2-(2-fluoroethoxy)benzyl)-N-(4-phenoxypyridin-3-yl)acetamide ([18F]FEPPA) (n=3), [18F]fluoroacetate ([18F]FAc) (n=3), and 18F-fluoro-2-deoxy-D-glucose ([18F]FDG) (n=3) were obtained at 0, 1, and 2 weeks after BDL. Biochemical assays, histological assays, immunohistochemical staining assays, and next generation sequencing analyses were also performed at 0 (n=3), 1 (n=3), 2 (n=3), and 3 (n=3) weeks after BDL, which demonstrated the severe damage in rat livers after BDL. Regarding [18F]FEPPA and [18F]FDG, there was a significantly higher uptake in the liver after BDL (both P<0.05), which lasted until week 2. However, the uptake of [18F]FAc in the liver was not significantly different before and after BDL (P=0.28). Collectively, both [18F]FEPPA and [18F]FDG can serve as sensitive probes for detecting the liver fibrosis. However, [18F]FAc is not recommended to diagnose liver fibrosis.http://dx.doi.org/10.1155/2021/7545284 |
| spellingShingle | Chun-Yi Wu Hsin-Hua Hsieh Pei-An Chu Wen-Hsiang Hong Ting-Yu Chang Chia-Fang Hsu Siao-Ting Lin Po-Hsun Su Shin-Lei Peng Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model Molecular Imaging |
| title | Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model |
| title_full | Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model |
| title_fullStr | Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model |
| title_full_unstemmed | Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model |
| title_short | Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model |
| title_sort | comparison of 18f fdg 18f fluoroacetate and 18f feppa for imaging liver fibrosis in a bile duct ligated rat model |
| url | http://dx.doi.org/10.1155/2021/7545284 |
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