Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line
Abstract Gene delivery has emerged as a novel and effective method in the treatment of malignancies within medical interventions by applying nanotechnology. Consequently, the development of appropriate nanocarriers is a key focus of this research. Dynamic light scattering (DLS), fourier transform in...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-11-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-78958-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846172137908338688 |
|---|---|
| author | Amirreza Diari Bidgoli Abbas Farmany Mohammad Taheri Meysam Soleimani Fatemeh Nouri |
| author_facet | Amirreza Diari Bidgoli Abbas Farmany Mohammad Taheri Meysam Soleimani Fatemeh Nouri |
| author_sort | Amirreza Diari Bidgoli |
| collection | DOAJ |
| description | Abstract Gene delivery has emerged as a novel and effective method in the treatment of malignancies within medical interventions by applying nanotechnology. Consequently, the development of appropriate nanocarriers is a key focus of this research. Dynamic light scattering (DLS), fourier transform infrared (FT-IR) spectroscopy, x-ray diffraction (XRD), and thermal gravimetric analysis (TGA) were employed for the characterization of the synthesized nanocarrier. Furthermore, to assess the gene transfer capability of the nanocarrier, various techniques such as gel retardation assay, nuclease resistance assay, cytotoxicity assay, flow cytometry, and transfection were employed. The average particle size and zeta potential of the GO-CS@Ca nanocarrier were obtained as 319.8 nm and + 92.8 mv, respectively. In the gel retardation test, it was observed that pDNA was effectively condensed by the GO-CS@Ca nanocarrier. The results of the MTT assay indicated that both GO-CS@Ca nanocarrier and the GO-CS@Ca/pDNA nanoplex with low toxicity. In flow cytometry analysis, it was observed that the complexation of pDNA with the GO-CS@Ca nanocarrier resulted in effective gene delivery to the MCF-7 cell line and consequently increased apoptosis induction. |
| format | Article |
| id | doaj-art-e07e371e312d49d080e03424bd1efb22 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e07e371e312d49d080e03424bd1efb222024-11-10T12:16:20ZengNature PortfolioScientific Reports2045-23222024-11-0114111110.1038/s41598-024-78958-3Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell lineAmirreza Diari Bidgoli0Abbas Farmany1Mohammad Taheri2Meysam Soleimani3Fatemeh Nouri4Department of Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical SciencesDental Implant Research Center, Dental School, Hamadan University of Medical SciencesDepartment of Medical Microbiology, School of Medicine, Hamadan University of Medical SciencesDepartment of Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical SciencesDepartment of Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical SciencesAbstract Gene delivery has emerged as a novel and effective method in the treatment of malignancies within medical interventions by applying nanotechnology. Consequently, the development of appropriate nanocarriers is a key focus of this research. Dynamic light scattering (DLS), fourier transform infrared (FT-IR) spectroscopy, x-ray diffraction (XRD), and thermal gravimetric analysis (TGA) were employed for the characterization of the synthesized nanocarrier. Furthermore, to assess the gene transfer capability of the nanocarrier, various techniques such as gel retardation assay, nuclease resistance assay, cytotoxicity assay, flow cytometry, and transfection were employed. The average particle size and zeta potential of the GO-CS@Ca nanocarrier were obtained as 319.8 nm and + 92.8 mv, respectively. In the gel retardation test, it was observed that pDNA was effectively condensed by the GO-CS@Ca nanocarrier. The results of the MTT assay indicated that both GO-CS@Ca nanocarrier and the GO-CS@Ca/pDNA nanoplex with low toxicity. In flow cytometry analysis, it was observed that the complexation of pDNA with the GO-CS@Ca nanocarrier resulted in effective gene delivery to the MCF-7 cell line and consequently increased apoptosis induction.https://doi.org/10.1038/s41598-024-78958-3Graphene-oxideChitosanBreast cancerMCF-7 cell lineGene deliveryNanocarrier |
| spellingShingle | Amirreza Diari Bidgoli Abbas Farmany Mohammad Taheri Meysam Soleimani Fatemeh Nouri Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line Scientific Reports Graphene-oxide Chitosan Breast cancer MCF-7 cell line Gene delivery Nanocarrier |
| title | Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line |
| title_full | Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line |
| title_fullStr | Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line |
| title_full_unstemmed | Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line |
| title_short | Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line |
| title_sort | preparation and characterization of calcium doped graphene oxide chitosan nanocarrier to enhance the gene delivery in mcf 7 cell line |
| topic | Graphene-oxide Chitosan Breast cancer MCF-7 cell line Gene delivery Nanocarrier |
| url | https://doi.org/10.1038/s41598-024-78958-3 |
| work_keys_str_mv | AT amirrezadiaribidgoli preparationandcharacterizationofcalciumdopedgrapheneoxidechitosannanocarriertoenhancethegenedeliveryinmcf7cellline AT abbasfarmany preparationandcharacterizationofcalciumdopedgrapheneoxidechitosannanocarriertoenhancethegenedeliveryinmcf7cellline AT mohammadtaheri preparationandcharacterizationofcalciumdopedgrapheneoxidechitosannanocarriertoenhancethegenedeliveryinmcf7cellline AT meysamsoleimani preparationandcharacterizationofcalciumdopedgrapheneoxidechitosannanocarriertoenhancethegenedeliveryinmcf7cellline AT fatemehnouri preparationandcharacterizationofcalciumdopedgrapheneoxidechitosannanocarriertoenhancethegenedeliveryinmcf7cellline |