Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection
Stripe rust of wheat is a serious disease caused by Puccinia striiformis f. sp. tritici (Pst). Growing resistant cultivars is the most preferred approach to control the disease. To identify wheat genotypes with quantitative trait loci (QTL) for durable resistance to stripe rust, 465 winter wheat ent...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1514926/full |
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| author | Fangjie Yao Fangjie Yao Meinan Wang Deven R. See Deven R. See Ennian Yang Guoyue Chen Guoyue Chen Xianming Chen Xianming Chen |
| author_facet | Fangjie Yao Fangjie Yao Meinan Wang Deven R. See Deven R. See Ennian Yang Guoyue Chen Guoyue Chen Xianming Chen Xianming Chen |
| author_sort | Fangjie Yao |
| collection | DOAJ |
| description | Stripe rust of wheat is a serious disease caused by Puccinia striiformis f. sp. tritici (Pst). Growing resistant cultivars is the most preferred approach to control the disease. To identify wheat genotypes with quantitative trait loci (QTL) for durable resistance to stripe rust, 465 winter wheat entries that were presumed to have high-temperature adult-plant (HTAP) resistance were used in this study. In the greenhouse seedling tests with seven Pst races, 16 entries were resistant to all the tested races. The 465 entries were also phenotyped for stripe rust responses at the adult-plant stage under natural infection of Pst in multiple field locations from 2018 to 2021 in the Washington state, and 345 entries were found to have stable resistance. The contrast of the susceptibility in the greenhouse seedling tests and the resistance in the field adult-plant stage for most of the entries indicated predominantly HTAP resistance in this panel. The durability of the resistance was demonstrated by a subset of 175 entries that were tested in multiple locations from 2007 to 2021. The 465 entries were genotyped through genotyping by multiplexed sequencing of single-nucleotide polymorphism (SNP) markers. Combining the stripe rust response and SNP marker data, a genome-wide association study (GWAS) was conducted, resulting in 143 marker–trait associations, from which 28 QTL that were detected at least with two races or in two field environments were identified, including seven for all-stage resistance and 21 for HTAP resistance. These QTL each explained 6.0% to 40.0% of the phenotypic variation. Compared with previously reported Yr genes and QTL based on their genomic positions, five QTL including two for HTAP resistance were identified as new. A total of 10 user-friendly Kompetitive allele specific PCR (KASP) markers were developed for eight of the HTAP resistance loci. In addition, molecular markers were used to detect 13 previously reported HTAP resistance genes/QTL, including two also identified in the GWAS analyses, and their frequencies ranged from 0.86% to 88.17% in the panel. The durable resistant genotypes, the genes/QTL identified, and the KASP markers developed in this study should be useful to develop wheat cultivars with long-lasting resistance to stripe rust. |
| format | Article |
| id | doaj-art-e05ab22493da427db9d547649bfead92 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-e05ab22493da427db9d547649bfead922025-01-07T06:48:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15149261514926Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detectionFangjie Yao0Fangjie Yao1Meinan Wang2Deven R. See3Deven R. See4Ennian Yang5Guoyue Chen6Guoyue Chen7Xianming Chen8Xianming Chen9Environment Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Key Laboratory of Wheat Biology and Genetic Improvement in Southwestern China, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, ChinaDepartment of Plant Pathology, Washington State University, Pullman, WA, United StatesDepartment of Plant Pathology, Washington State University, Pullman, WA, United StatesDepartment of Plant Pathology, Washington State University, Pullman, WA, United StatesUS Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics, and Quality Research Unit, Pullman, WA, United StatesEnvironment Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Key Laboratory of Wheat Biology and Genetic Improvement in Southwestern China, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, ChinaState Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Plant Pathology, Washington State University, Pullman, WA, United StatesUS Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics, and Quality Research Unit, Pullman, WA, United StatesStripe rust of wheat is a serious disease caused by Puccinia striiformis f. sp. tritici (Pst). Growing resistant cultivars is the most preferred approach to control the disease. To identify wheat genotypes with quantitative trait loci (QTL) for durable resistance to stripe rust, 465 winter wheat entries that were presumed to have high-temperature adult-plant (HTAP) resistance were used in this study. In the greenhouse seedling tests with seven Pst races, 16 entries were resistant to all the tested races. The 465 entries were also phenotyped for stripe rust responses at the adult-plant stage under natural infection of Pst in multiple field locations from 2018 to 2021 in the Washington state, and 345 entries were found to have stable resistance. The contrast of the susceptibility in the greenhouse seedling tests and the resistance in the field adult-plant stage for most of the entries indicated predominantly HTAP resistance in this panel. The durability of the resistance was demonstrated by a subset of 175 entries that were tested in multiple locations from 2007 to 2021. The 465 entries were genotyped through genotyping by multiplexed sequencing of single-nucleotide polymorphism (SNP) markers. Combining the stripe rust response and SNP marker data, a genome-wide association study (GWAS) was conducted, resulting in 143 marker–trait associations, from which 28 QTL that were detected at least with two races or in two field environments were identified, including seven for all-stage resistance and 21 for HTAP resistance. These QTL each explained 6.0% to 40.0% of the phenotypic variation. Compared with previously reported Yr genes and QTL based on their genomic positions, five QTL including two for HTAP resistance were identified as new. A total of 10 user-friendly Kompetitive allele specific PCR (KASP) markers were developed for eight of the HTAP resistance loci. In addition, molecular markers were used to detect 13 previously reported HTAP resistance genes/QTL, including two also identified in the GWAS analyses, and their frequencies ranged from 0.86% to 88.17% in the panel. The durable resistant genotypes, the genes/QTL identified, and the KASP markers developed in this study should be useful to develop wheat cultivars with long-lasting resistance to stripe rust.https://www.frontiersin.org/articles/10.3389/fpls.2024.1514926/fullwheatstripe rustdurable resistancegenome-wide association studymarker-assisted detection |
| spellingShingle | Fangjie Yao Fangjie Yao Meinan Wang Deven R. See Deven R. See Ennian Yang Guoyue Chen Guoyue Chen Xianming Chen Xianming Chen Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection Frontiers in Plant Science wheat stripe rust durable resistance genome-wide association study marker-assisted detection |
| title | Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection |
| title_full | Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection |
| title_fullStr | Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection |
| title_full_unstemmed | Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection |
| title_short | Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection |
| title_sort | identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high temperature adult plant resistance through genome wide association mapping and marker assisted detection |
| topic | wheat stripe rust durable resistance genome-wide association study marker-assisted detection |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1514926/full |
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