Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance
Introduction: Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. Methodology: Serum samples were collecte...
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The Journal of Infection in Developing Countries
2011-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/1339 |
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| author | Moustafa Abdel Fadeel Brent L House Momtaz M Wasfy John D Klena Engy E Habashy Mayar M Said Mohamed Abdel Maksoud Bassem Abdel Rahman Guillermo Pimentel |
| author_facet | Moustafa Abdel Fadeel Brent L House Momtaz M Wasfy John D Klena Engy E Habashy Mayar M Said Mohamed Abdel Maksoud Bassem Abdel Rahman Guillermo Pimentel |
| author_sort | Moustafa Abdel Fadeel |
| collection | DOAJ |
| description |
Introduction: Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations.
Methodology: Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses a mixture of somatic and flagellar antigens to detect the total antibody response against Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) infection. The levels of Ig isotype response (IgG, IgM and IgA) were also evaluated, and results were compared to those of TUBEX-TF and Typhidot commercial kits.
Results: Of 234 culture-confirmed typhoid patients, the total Ig ELISA diagnosed 93% compared to 71% using Widal test. This sensitivity level (93%) is higher than that observed for the individual Ig ELISAs (IgG 75%; IgM 79%; IgA 57%) and the commercial tests TUBEX-TF (75%), Typhidot IgM (63%) and Typhidot IgG (28%). An agreement of 78% was achieved between the total Ig ELISA and Widal test. The average specificity of the ELISA was 96%. Using ELISA, up to 200 samples can be tested per run with cost per test at US$0.20.
Conclusions: The developed ELISA shows superior sensitivity and specificity, when compared to Widal, TUBEX-TF and Typhidot assays, is more cost effective and allows higher throughput. This method is highly recommended for active surveillance studies or outbreak investigations of typhoid fever.
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| format | Article |
| id | doaj-art-dfd8957bf57e46cf8415a0d9df1eacf8 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2011-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-dfd8957bf57e46cf8415a0d9df1eacf82025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802011-03-0150310.3855/jidc.1339Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillanceMoustafa Abdel Fadeel0Brent L House1Momtaz M Wasfy2John D Klena3Engy E Habashy4Mayar M Said5Mohamed Abdel Maksoud6Bassem Abdel Rahman7Guillermo Pimentel8US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, EgyptUS Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt Introduction: Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. Methodology: Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses a mixture of somatic and flagellar antigens to detect the total antibody response against Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) infection. The levels of Ig isotype response (IgG, IgM and IgA) were also evaluated, and results were compared to those of TUBEX-TF and Typhidot commercial kits. Results: Of 234 culture-confirmed typhoid patients, the total Ig ELISA diagnosed 93% compared to 71% using Widal test. This sensitivity level (93%) is higher than that observed for the individual Ig ELISAs (IgG 75%; IgM 79%; IgA 57%) and the commercial tests TUBEX-TF (75%), Typhidot IgM (63%) and Typhidot IgG (28%). An agreement of 78% was achieved between the total Ig ELISA and Widal test. The average specificity of the ELISA was 96%. Using ELISA, up to 200 samples can be tested per run with cost per test at US$0.20. Conclusions: The developed ELISA shows superior sensitivity and specificity, when compared to Widal, TUBEX-TF and Typhidot assays, is more cost effective and allows higher throughput. This method is highly recommended for active surveillance studies or outbreak investigations of typhoid fever. https://jidc.org/index.php/journal/article/view/1339typhoidserological diagnosisELISAsurveillance |
| spellingShingle | Moustafa Abdel Fadeel Brent L House Momtaz M Wasfy John D Klena Engy E Habashy Mayar M Said Mohamed Abdel Maksoud Bassem Abdel Rahman Guillermo Pimentel Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance Journal of Infection in Developing Countries typhoid serological diagnosis ELISA surveillance |
| title | Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance |
| title_full | Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance |
| title_fullStr | Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance |
| title_full_unstemmed | Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance |
| title_short | Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance |
| title_sort | evaluation of a newly developed elisa against widal tubex tf and typhidot for typhoid fever surveillance |
| topic | typhoid serological diagnosis ELISA surveillance |
| url | https://jidc.org/index.php/journal/article/view/1339 |
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