EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cells
Abstract Objectives The study aimed to explore the effects of EGb761 on vascular dementia (VD) rats and the mechanisms of action. Methods The Morris water maze test was utilized to assess the spatial learning and memory abilities of the rats; Hematoxylin and Eosin (HE) staining and electron microsco...
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| Language: | English |
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BMC
2025-06-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02681-6 |
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| author | Nan Yin Zhipeng Tang Yanyan Yang Xiuqin Li Ruisheng Duan Guodong Xu Peiyuan Lv |
| author_facet | Nan Yin Zhipeng Tang Yanyan Yang Xiuqin Li Ruisheng Duan Guodong Xu Peiyuan Lv |
| author_sort | Nan Yin |
| collection | DOAJ |
| description | Abstract Objectives The study aimed to explore the effects of EGb761 on vascular dementia (VD) rats and the mechanisms of action. Methods The Morris water maze test was utilized to assess the spatial learning and memory abilities of the rats; Hematoxylin and Eosin (HE) staining and electron microscopy were used to observe changes in hippocampal neuron cells; Immunohistochemistry was performed to detect the expression of cleaved caspase-3 and microtubule-associated proteins light chain 3 (LC3-II) positive cells in hippocampal neurons; immunofluorescence staining was carried out to determine the immunofluorescence intensity of IRGM in hippocampal neurons; western blotting was used to measure the expression of related proteins. Results EGb761 significantly improved the cognitive function of vascular dementia rats (P < 0.01) and reduced the apoptosis of hippocampal neurons.Furthermore, EGb761 suppressed ROS, thereby promoting the expression of proteins related to the Wnt/β-catenin signaling pathway and inhibiting the expression of C-Jun N-terminal Kinase (p-JNK), c-Jun N-terminal kinase (p-c-JUN), Protein 53 (P53), immunity-related GTPase M (IRGM), Transcription Factor EB (TFEB), microtubule-associated proteins light chain 3 (LC3), Lysosomal Associated Membrane Protein 1 (LAMP1), and Sequestosome 1 (SQSTM1). Conclusions Ginkgo Biloba Extract 761 (EGb761) mediated the Wnt/β-catenin signaling pathway to inhibit apoptosis and autophagy in hippocampal neurons in VD rats. |
| format | Article |
| id | doaj-art-df9bb058043b4f9296bde1035a88bc01 |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-df9bb058043b4f9296bde1035a88bc012025-08-20T03:47:24ZengBMCEuropean Journal of Medical Research2047-783X2025-06-0130111410.1186/s40001-025-02681-6EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cellsNan Yin0Zhipeng Tang1Yanyan Yang2Xiuqin Li3Ruisheng Duan4Guodong Xu5Peiyuan Lv6Department of Neurology, Hebei Medical UniversityDepartment of Laboratory Medicine, Hebei General HospitalDepartment of Gynecology, Hebei General HospitalDepartment of Geriatrics, Hebei General HospitalDepartment of Neurology, Hebei General HospitalDepartment of Neurology, Hebei General HospitalDepartment of Neurology, Hebei Medical UniversityAbstract Objectives The study aimed to explore the effects of EGb761 on vascular dementia (VD) rats and the mechanisms of action. Methods The Morris water maze test was utilized to assess the spatial learning and memory abilities of the rats; Hematoxylin and Eosin (HE) staining and electron microscopy were used to observe changes in hippocampal neuron cells; Immunohistochemistry was performed to detect the expression of cleaved caspase-3 and microtubule-associated proteins light chain 3 (LC3-II) positive cells in hippocampal neurons; immunofluorescence staining was carried out to determine the immunofluorescence intensity of IRGM in hippocampal neurons; western blotting was used to measure the expression of related proteins. Results EGb761 significantly improved the cognitive function of vascular dementia rats (P < 0.01) and reduced the apoptosis of hippocampal neurons.Furthermore, EGb761 suppressed ROS, thereby promoting the expression of proteins related to the Wnt/β-catenin signaling pathway and inhibiting the expression of C-Jun N-terminal Kinase (p-JNK), c-Jun N-terminal kinase (p-c-JUN), Protein 53 (P53), immunity-related GTPase M (IRGM), Transcription Factor EB (TFEB), microtubule-associated proteins light chain 3 (LC3), Lysosomal Associated Membrane Protein 1 (LAMP1), and Sequestosome 1 (SQSTM1). Conclusions Ginkgo Biloba Extract 761 (EGb761) mediated the Wnt/β-catenin signaling pathway to inhibit apoptosis and autophagy in hippocampal neurons in VD rats.https://doi.org/10.1186/s40001-025-02681-6Vascular dementia ratsHippocampal neuronsEGb761Wnt/β-catenin signaling pathwayAutophagy |
| spellingShingle | Nan Yin Zhipeng Tang Yanyan Yang Xiuqin Li Ruisheng Duan Guodong Xu Peiyuan Lv EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cells European Journal of Medical Research Vascular dementia rats Hippocampal neurons EGb761 Wnt/β-catenin signaling pathway Autophagy |
| title | EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cells |
| title_full | EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cells |
| title_fullStr | EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cells |
| title_full_unstemmed | EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cells |
| title_short | EGb761 suppressed vascular dementia via modulating Wnt/β-catenin signaling pathway-induced apoptosis and autophagy in hippocampal neuronal cells |
| title_sort | egb761 suppressed vascular dementia via modulating wnt β catenin signaling pathway induced apoptosis and autophagy in hippocampal neuronal cells |
| topic | Vascular dementia rats Hippocampal neurons EGb761 Wnt/β-catenin signaling pathway Autophagy |
| url | https://doi.org/10.1186/s40001-025-02681-6 |
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