Labeling Human Melanoma Cells With SPIO
Objectives: To use the superparamagnetic iron oxide (SPIO) contrast agent Resovist (±transfection agent) to label human melanoma cells and determine its effects on cellular viability, microstructure, iron quantity, and magnetic resonance imaging (MRI) detectability. Materials and Methods: Human SK-M...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2016-01-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012115624915 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841563178637459456 |
|---|---|
| author | Daniel Spira MD Rüdiger Bantleon PhD Hartwig Wolburg PhD Fritz Schick MD, PhD Gerd Groezinger MD Jakub Wiskirchen MD Benjamin Wiesinger MD |
| author_facet | Daniel Spira MD Rüdiger Bantleon PhD Hartwig Wolburg PhD Fritz Schick MD, PhD Gerd Groezinger MD Jakub Wiskirchen MD Benjamin Wiesinger MD |
| author_sort | Daniel Spira MD |
| collection | DOAJ |
| description | Objectives: To use the superparamagnetic iron oxide (SPIO) contrast agent Resovist (±transfection agent) to label human melanoma cells and determine its effects on cellular viability, microstructure, iron quantity, and magnetic resonance imaging (MRI) detectability. Materials and Methods: Human SK-Mel28 melanoma cells were incubated with Resovist (±liposomal transfection agent DOSPER). The cellular iron content was measured, and labeled cells were examined at 1.5 T and 3.0 T. The intracellular and extracellular distributions of the contrast agent were assessed by light and electron microscopy. Results: The incubation of melanoma cells with SPIO does not interfere with cell viability or proliferation. The iron is located both intracellularly and extracellularly as iron clusters associated with the exterior of the cell membrane. Despite thorough washing, the extracellular SPIO remained associated with the cell membrane. The liposomal transfection agent does not change the maximum achievable cellular iron content but promotes a faster iron uptake. The MRI detectability persists for at least 7 days. Conclusion: The transfection agent DOSPER facilitates the efficient labeling of human metastatic melanoma cells with Resovist. Our findings raise the possibility that other Resovist-labeled cells may collect associated extracellular nanoparticles. The SPIO may be available to other iron-handling cells and not completely compartmentalized during the labeling procedure. |
| format | Article |
| id | doaj-art-df7f0178db39470ea0414093dccbc0b0 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-df7f0178db39470ea0414093dccbc0b02025-01-03T00:10:41ZengSAGE PublishingMolecular Imaging1536-01212016-01-011510.1177/153601211562491510.1177_1536012115624915Labeling Human Melanoma Cells With SPIODaniel Spira MD0Rüdiger Bantleon PhD1Hartwig Wolburg PhD2Fritz Schick MD, PhD3Gerd Groezinger MD4Jakub Wiskirchen MD5Benjamin Wiesinger MD6Department of Diagnostic and Interventional Radiology, Universitätsklinikum Heidelberg, Im Neuenheimer Feld 110, 69120 HeidelbergDepartment of Diagnostic and Interventional Radiology, Eberhard-Karls-University Tübingen, Hoppe-Seyler-Str. 3, 72076 Tübingen, GermanyInstitute of Pathology and Neuropathology, Eberhard-Karls-University Tübingen, Liebermeisterstraße 8, 72076 Tübingen, GermanySection on Experimental Radiology, Eberhard-Karls-University Tübingen, Hoppe-Seyler-Str. 3, 72076 Tübingen, GermanyDepartment of Diagnostic and Interventional Radiology, Eberhard-Karls-University Tübingen, Hoppe-Seyler-Str. 3, 72076 Tübingen, GermanyDepartment of Radiology and Nuclear Medicine, Franziskus Hospital Bielefeld, Kiskerstraße 26, 33615 Bielefeld, GermanyDepartment of Diagnostic and Interventional Radiology, Eberhard-Karls-University Tübingen, Hoppe-Seyler-Str. 3, 72076 Tübingen, GermanyObjectives: To use the superparamagnetic iron oxide (SPIO) contrast agent Resovist (±transfection agent) to label human melanoma cells and determine its effects on cellular viability, microstructure, iron quantity, and magnetic resonance imaging (MRI) detectability. Materials and Methods: Human SK-Mel28 melanoma cells were incubated with Resovist (±liposomal transfection agent DOSPER). The cellular iron content was measured, and labeled cells were examined at 1.5 T and 3.0 T. The intracellular and extracellular distributions of the contrast agent were assessed by light and electron microscopy. Results: The incubation of melanoma cells with SPIO does not interfere with cell viability or proliferation. The iron is located both intracellularly and extracellularly as iron clusters associated with the exterior of the cell membrane. Despite thorough washing, the extracellular SPIO remained associated with the cell membrane. The liposomal transfection agent does not change the maximum achievable cellular iron content but promotes a faster iron uptake. The MRI detectability persists for at least 7 days. Conclusion: The transfection agent DOSPER facilitates the efficient labeling of human metastatic melanoma cells with Resovist. Our findings raise the possibility that other Resovist-labeled cells may collect associated extracellular nanoparticles. The SPIO may be available to other iron-handling cells and not completely compartmentalized during the labeling procedure.https://doi.org/10.1177/1536012115624915 |
| spellingShingle | Daniel Spira MD Rüdiger Bantleon PhD Hartwig Wolburg PhD Fritz Schick MD, PhD Gerd Groezinger MD Jakub Wiskirchen MD Benjamin Wiesinger MD Labeling Human Melanoma Cells With SPIO Molecular Imaging |
| title | Labeling Human Melanoma Cells With SPIO |
| title_full | Labeling Human Melanoma Cells With SPIO |
| title_fullStr | Labeling Human Melanoma Cells With SPIO |
| title_full_unstemmed | Labeling Human Melanoma Cells With SPIO |
| title_short | Labeling Human Melanoma Cells With SPIO |
| title_sort | labeling human melanoma cells with spio |
| url | https://doi.org/10.1177/1536012115624915 |
| work_keys_str_mv | AT danielspiramd labelinghumanmelanomacellswithspio AT rudigerbantleonphd labelinghumanmelanomacellswithspio AT hartwigwolburgphd labelinghumanmelanomacellswithspio AT fritzschickmdphd labelinghumanmelanomacellswithspio AT gerdgroezingermd labelinghumanmelanomacellswithspio AT jakubwiskirchenmd labelinghumanmelanomacellswithspio AT benjaminwiesingermd labelinghumanmelanomacellswithspio |