Inflammatory PDLSCs promotes T cell migration and activation: A pilot study
[Objective:] To explore the inflammatory periodontal ligament stem cells (PDLSCs) on T cell immunity in periodontitis conditions and its possible mechanism. [Methods:] PDLSCs were stimulated with phosphate buffered saline （PBS) and lipopolysaccharide (LPS) to mimic PDLSCs in healthy and inflammatory...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-06-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.003 |
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| _version_ | 1849225138383355904 |
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| author | REN Le WEI Yu MA Pengfei YI Zimei ZHAO Jiechen WU Junhua |
| author_facet | REN Le WEI Yu MA Pengfei YI Zimei ZHAO Jiechen WU Junhua |
| author_sort | REN Le |
| collection | DOAJ |
| description | [Objective:] To explore the inflammatory periodontal ligament stem cells (PDLSCs) on T cell immunity in periodontitis conditions and its possible mechanism. [Methods:] PDLSCs were stimulated with phosphate buffered saline （PBS) and lipopolysaccharide (LPS) to mimic PDLSCs in healthy and inflammatory environments, and then the two groups of conditioned medium (CM) were collected, namely healthy PDLSCs CM (CON‐CM group) and inflammatory PDLSCs CM (LPS‐CM group). The same batch of culture medium was collected as the negative control (NC group) as well. They were used to act on human normal T lymphocyte line H9 cells respectively to observe the effects of different CM on T cell proliferation and migration. Furthermore, phytohemagglutinin‐L (PHA‐L) was used to stimulate T cell immune activation, and CM was used to treat H9 cells in the activated state, then the expression of T cell immune activation related genes—interleukin‐2 (IL‐2), interferon‐γ (IFN‐γ), interleukin‐17 (IL‐17) and CC chemokine receptor 6 (CCR6) were detected, and the effect of LPS stimulation on the secretion of CC chemokine ligand 20 (CCL20) by PDLSCs was observed. [Results:] Compared with the NC group and the CON‐CM group, the LPS‐CM group could promote the proliferation and migration of T cells, as well as the expression of IL‐2, IFN‐γ, IL‐17 and CCR6 mRNA (P<0.05). While the content of CCL20 secreted by PDLSCs was significantly increased under LPS stimulation (P<0.001). [Conclusion:] This study provides evidence that normal PDLSCs have certain immunomodulatory effects, while inflammatory PDLSCs may promote T cell proliferation, migration, activation and other immunobiological behaviors through the CCL20‐CCR6 axis. |
| format | Article |
| id | doaj-art-df5002def58d4b71b08c48ab13b97c0b |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2023-06-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-df5002def58d4b71b08c48ab13b97c0b2025-08-25T06:10:06ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792023-06-0133314815410.3969/j.issn.1005-4979.2023.03.003Inflammatory PDLSCs promotes T cell migration and activation: A pilot studyREN Le0WEI Yu1MA Pengfei2YI Zimei3ZHAO Jiechen4WU Junhua5Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China[Objective:] To explore the inflammatory periodontal ligament stem cells (PDLSCs) on T cell immunity in periodontitis conditions and its possible mechanism. [Methods:] PDLSCs were stimulated with phosphate buffered saline （PBS) and lipopolysaccharide (LPS) to mimic PDLSCs in healthy and inflammatory environments, and then the two groups of conditioned medium (CM) were collected, namely healthy PDLSCs CM (CON‐CM group) and inflammatory PDLSCs CM (LPS‐CM group). The same batch of culture medium was collected as the negative control (NC group) as well. They were used to act on human normal T lymphocyte line H9 cells respectively to observe the effects of different CM on T cell proliferation and migration. Furthermore, phytohemagglutinin‐L (PHA‐L) was used to stimulate T cell immune activation, and CM was used to treat H9 cells in the activated state, then the expression of T cell immune activation related genes—interleukin‐2 (IL‐2), interferon‐γ (IFN‐γ), interleukin‐17 (IL‐17) and CC chemokine receptor 6 (CCR6) were detected, and the effect of LPS stimulation on the secretion of CC chemokine ligand 20 (CCL20) by PDLSCs was observed. [Results:] Compared with the NC group and the CON‐CM group, the LPS‐CM group could promote the proliferation and migration of T cells, as well as the expression of IL‐2, IFN‐γ, IL‐17 and CCR6 mRNA (P<0.05). While the content of CCL20 secreted by PDLSCs was significantly increased under LPS stimulation (P<0.001). [Conclusion:] This study provides evidence that normal PDLSCs have certain immunomodulatory effects, while inflammatory PDLSCs may promote T cell proliferation, migration, activation and other immunobiological behaviors through the CCL20‐CCR6 axis.https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.003periodontitisperiodontal ligament stem cellst cell immunitycc chemokine ligand 20cc chemokine receptor 6 |
| spellingShingle | REN Le WEI Yu MA Pengfei YI Zimei ZHAO Jiechen WU Junhua Inflammatory PDLSCs promotes T cell migration and activation: A pilot study Kouqiang hemian waike zazhi periodontitis periodontal ligament stem cells t cell immunity cc chemokine ligand 20 cc chemokine receptor 6 |
| title | Inflammatory PDLSCs promotes T cell migration and activation: A pilot study |
| title_full | Inflammatory PDLSCs promotes T cell migration and activation: A pilot study |
| title_fullStr | Inflammatory PDLSCs promotes T cell migration and activation: A pilot study |
| title_full_unstemmed | Inflammatory PDLSCs promotes T cell migration and activation: A pilot study |
| title_short | Inflammatory PDLSCs promotes T cell migration and activation: A pilot study |
| title_sort | inflammatory pdlscs promotes t cell migration and activation a pilot study |
| topic | periodontitis periodontal ligament stem cells t cell immunity cc chemokine ligand 20 cc chemokine receptor 6 |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.003 |
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