Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch
Abstract Background The use of light to control the activity of living cells is a promising approach in cardiac research due to its unparalleled spatio-temporal selectivity and minimal invasiveness. Ziapin2, a newly synthesized azobenzene compound, has recently been reported as an efficient tool for...
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| Format: | Article |
| Language: | English |
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BMC
2024-11-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-024-05902-4 |
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| author | Chiara Florindi Vito Vurro Paola Moretti Chiara Bertarelli Antonio Zaza Guglielmo Lanzani Francesco Lodola |
| author_facet | Chiara Florindi Vito Vurro Paola Moretti Chiara Bertarelli Antonio Zaza Guglielmo Lanzani Francesco Lodola |
| author_sort | Chiara Florindi |
| collection | DOAJ |
| description | Abstract Background The use of light to control the activity of living cells is a promising approach in cardiac research due to its unparalleled spatio-temporal selectivity and minimal invasiveness. Ziapin2, a newly synthesized azobenzene compound, has recently been reported as an efficient tool for light-driven modulation of excitation-contraction coupling (ECC) in human-induced pluripotent stem cells–derived cardiomyocytes. However, the exact biophysical mechanism of this process remains incompletely understood. Methods To address this, we performed a detailed electrophysiological characterization in a more mature cardiac model, specifically adult mouse ventricular myocytes (AMVMs). Results Our in vitro results demonstrate that Ziapin2 can photomodulate cardiac ECC in mature AMVMs without affecting the main transporters and receptors located within the sarcolemma. We established a connection between Ziapin2-induced membrane thickness modulation and light-generated action potentials by showcasing the pivotal role of stretch-activated channels (SACs). Notably, our experimental findings, through pharmacological blockade, suggest that non-selective SACs might serve as the biological culprit responsible for the effect. Conclusions Taken together, these findings elucidate the intricacies of Ziapin2-mediated photostimulation mechanism and open new perspectives for its application in cardiac research. |
| format | Article |
| id | doaj-art-df3124a47a35433495c4e2113205fc32 |
| institution | Kabale University |
| issn | 1479-5876 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-df3124a47a35433495c4e2113205fc322024-12-01T12:42:18ZengBMCJournal of Translational Medicine1479-58762024-11-0122111210.1186/s12967-024-05902-4Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitchChiara Florindi0Vito Vurro1Paola Moretti2Chiara Bertarelli3Antonio Zaza4Guglielmo Lanzani5Francesco Lodola6Department of Biotechnology and Biosciences, University of Milano-BicoccaCenter for Nano Science and Technology, Istituto Italiano di TecnologiaCenter for Nano Science and Technology, Istituto Italiano di TecnologiaCenter for Nano Science and Technology, Istituto Italiano di TecnologiaDepartment of Biotechnology and Biosciences, University of Milano-BicoccaCenter for Nano Science and Technology, Istituto Italiano di TecnologiaDepartment of Biotechnology and Biosciences, University of Milano-BicoccaAbstract Background The use of light to control the activity of living cells is a promising approach in cardiac research due to its unparalleled spatio-temporal selectivity and minimal invasiveness. Ziapin2, a newly synthesized azobenzene compound, has recently been reported as an efficient tool for light-driven modulation of excitation-contraction coupling (ECC) in human-induced pluripotent stem cells–derived cardiomyocytes. However, the exact biophysical mechanism of this process remains incompletely understood. Methods To address this, we performed a detailed electrophysiological characterization in a more mature cardiac model, specifically adult mouse ventricular myocytes (AMVMs). Results Our in vitro results demonstrate that Ziapin2 can photomodulate cardiac ECC in mature AMVMs without affecting the main transporters and receptors located within the sarcolemma. We established a connection between Ziapin2-induced membrane thickness modulation and light-generated action potentials by showcasing the pivotal role of stretch-activated channels (SACs). Notably, our experimental findings, through pharmacological blockade, suggest that non-selective SACs might serve as the biological culprit responsible for the effect. Conclusions Taken together, these findings elucidate the intricacies of Ziapin2-mediated photostimulation mechanism and open new perspectives for its application in cardiac research.https://doi.org/10.1186/s12967-024-05902-4Light stimulationMolecular photoswitchesCardiac cell modulationStretch-activated channels |
| spellingShingle | Chiara Florindi Vito Vurro Paola Moretti Chiara Bertarelli Antonio Zaza Guglielmo Lanzani Francesco Lodola Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch Journal of Translational Medicine Light stimulation Molecular photoswitches Cardiac cell modulation Stretch-activated channels |
| title | Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch |
| title_full | Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch |
| title_fullStr | Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch |
| title_full_unstemmed | Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch |
| title_short | Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch |
| title_sort | role of stretch activated channels in light generated action potentials mediated by an intramembrane molecular photoswitch |
| topic | Light stimulation Molecular photoswitches Cardiac cell modulation Stretch-activated channels |
| url | https://doi.org/10.1186/s12967-024-05902-4 |
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