Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma
Abstract PrPc is expressed in various tumors and is associated with cancer progression, but previous studies have shown conflicting results regarding its relationship with patient prognosis—potentially due to differences in the antibodies used. This study aimed to clarify the relationship between Pr...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-82398-4 |
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| author | Cheng Zhang Fanlei Ran Lei Du Yang Cao Hong Chen Quan Chen Lijun Bi Haiying Hang |
| author_facet | Cheng Zhang Fanlei Ran Lei Du Yang Cao Hong Chen Quan Chen Lijun Bi Haiying Hang |
| author_sort | Cheng Zhang |
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| description | Abstract PrPc is expressed in various tumors and is associated with cancer progression, but previous studies have shown conflicting results regarding its relationship with patient prognosis—potentially due to differences in the antibodies used. This study aimed to clarify the relationship between PrPc expression and primary esophageal squamous cell carcinoma (ESCC) and primary hepatocellular carcinoma (HCC) using a novel anti-PrPc antibody, 4AA-m, noted for its high specificity and sensitivity. We used flow cytometry to detect PrPc expression in ESCC and HCC cell lines. Immunohistochemistry with 4AA-m was then performed on tissue microarrays from 179 patients with primary ESCC and 92 patients with primary HCC. PrPc expression was semi-quantitatively assessed using the Tumor-DAB-H-Score, and its association with tumor prognosis was analyzed. In ESCC, PrPc expression was negatively correlated with lymph node metastasis, and patients with high PrPc expression had better overall survival compared to those with low expression. PrPc expression was identified as an independent prognostic factor for overall survival in ESCC. In HCC, patients with positive PrPc expression had shorter recurrence-free survival (RFS) than those without PrPc expression. PrPc expression was also found to be an independent prognostic factor for RFS in HCC. |
| format | Article |
| id | doaj-art-df149d8ccd08419cafeea6cd1bb7cd8b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-df149d8ccd08419cafeea6cd1bb7cd8b2024-12-29T12:24:40ZengNature PortfolioScientific Reports2045-23222024-12-0114111210.1038/s41598-024-82398-4Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinomaCheng Zhang0Fanlei Ran1Lei Du2Yang Cao3Hong Chen4Quan Chen5Lijun Bi6Haiying Hang7Institute of Biophysics, Chinese Academy of SciencesInstitute of Biophysics, Chinese Academy of SciencesThe State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of SciencesCenter of Growth, Metabolism and Aging, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan UniversityInstitute of Biophysics, Chinese Academy of SciencesThe State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai UniversityInstitute of Biophysics, Chinese Academy of SciencesInstitute of Biophysics, Chinese Academy of SciencesAbstract PrPc is expressed in various tumors and is associated with cancer progression, but previous studies have shown conflicting results regarding its relationship with patient prognosis—potentially due to differences in the antibodies used. This study aimed to clarify the relationship between PrPc expression and primary esophageal squamous cell carcinoma (ESCC) and primary hepatocellular carcinoma (HCC) using a novel anti-PrPc antibody, 4AA-m, noted for its high specificity and sensitivity. We used flow cytometry to detect PrPc expression in ESCC and HCC cell lines. Immunohistochemistry with 4AA-m was then performed on tissue microarrays from 179 patients with primary ESCC and 92 patients with primary HCC. PrPc expression was semi-quantitatively assessed using the Tumor-DAB-H-Score, and its association with tumor prognosis was analyzed. In ESCC, PrPc expression was negatively correlated with lymph node metastasis, and patients with high PrPc expression had better overall survival compared to those with low expression. PrPc expression was identified as an independent prognostic factor for overall survival in ESCC. In HCC, patients with positive PrPc expression had shorter recurrence-free survival (RFS) than those without PrPc expression. PrPc expression was also found to be an independent prognostic factor for RFS in HCC.https://doi.org/10.1038/s41598-024-82398-4Cellular prion proteinPrimary esophageal squamous cell carcinomaPrimary hepatocellular carcinomaPrognosisLymph node metastasis |
| spellingShingle | Cheng Zhang Fanlei Ran Lei Du Yang Cao Hong Chen Quan Chen Lijun Bi Haiying Hang Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma Scientific Reports Cellular prion protein Primary esophageal squamous cell carcinoma Primary hepatocellular carcinoma Prognosis Lymph node metastasis |
| title | Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma |
| title_full | Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma |
| title_fullStr | Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma |
| title_full_unstemmed | Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma |
| title_short | Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma |
| title_sort | re evaluation of the relationship between prpc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma |
| topic | Cellular prion protein Primary esophageal squamous cell carcinoma Primary hepatocellular carcinoma Prognosis Lymph node metastasis |
| url | https://doi.org/10.1038/s41598-024-82398-4 |
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