lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression
lncRNA is a key epigenetic regulator in biological processes. In the human cancer transcriptome library MiTranscriptome, we identified GAU1 as the top upregulated lncRNA in colorectal cancer (CRC) by sample set enrichment analysis (overexpression ranking percentile=99.75%, P<10−50), which is coex...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2021/5960821 |
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| author | Xuemei Tang Haoyu Ruan Liu Dong Sihan Li Zhiyuan Wu Ming Guan |
| author_facet | Xuemei Tang Haoyu Ruan Liu Dong Sihan Li Zhiyuan Wu Ming Guan |
| author_sort | Xuemei Tang |
| collection | DOAJ |
| description | lncRNA is a key epigenetic regulator in biological processes. In the human cancer transcriptome library MiTranscriptome, we identified GAU1 as the top upregulated lncRNA in colorectal cancer (CRC) by sample set enrichment analysis (overexpression ranking percentile=99.75%, P<10−50), which is coexpressed with the potential oncogene GALNT8 (Spearman rho=0.67, P=2.44×10−23, TCGA dataset n=184). Experimental data revealed that GAU1 regulates the expression of GALNT8. The overexpression of either GAU1 or GALNT8 significantly promotes the cell cycle and proliferation of CRC cell lines and correlates with poor prognosis in patients with CRC (P=3.04×10−2), while silencing of GAU1 or GALNT8 suppressed the cancer cell proliferation and induced the CRC cell line resistance to oxaliplatin in vitro treatment. Our results suggested that the previously less studied GAU1 and GALNT8 may play as CRC prognosis markers and potential targets for chemotherapy treatment. |
| format | Article |
| id | doaj-art-df0975b85f5747ac801abc3854dda14e |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-df0975b85f5747ac801abc3854dda14e2025-02-03T07:23:28ZengWileyGastroenterology Research and Practice1687-61211687-630X2021-01-01202110.1155/2021/59608215960821lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer ProgressionXuemei Tang0Haoyu Ruan1Liu Dong2Sihan Li3Zhiyuan Wu4Ming Guan5Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, ChinaCenter for Pharmacogenetics, Department of Pharmaceutical Sciences, University of Pittsburgh, PA 15261, USADepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, ChinalncRNA is a key epigenetic regulator in biological processes. In the human cancer transcriptome library MiTranscriptome, we identified GAU1 as the top upregulated lncRNA in colorectal cancer (CRC) by sample set enrichment analysis (overexpression ranking percentile=99.75%, P<10−50), which is coexpressed with the potential oncogene GALNT8 (Spearman rho=0.67, P=2.44×10−23, TCGA dataset n=184). Experimental data revealed that GAU1 regulates the expression of GALNT8. The overexpression of either GAU1 or GALNT8 significantly promotes the cell cycle and proliferation of CRC cell lines and correlates with poor prognosis in patients with CRC (P=3.04×10−2), while silencing of GAU1 or GALNT8 suppressed the cancer cell proliferation and induced the CRC cell line resistance to oxaliplatin in vitro treatment. Our results suggested that the previously less studied GAU1 and GALNT8 may play as CRC prognosis markers and potential targets for chemotherapy treatment.http://dx.doi.org/10.1155/2021/5960821 |
| spellingShingle | Xuemei Tang Haoyu Ruan Liu Dong Sihan Li Zhiyuan Wu Ming Guan lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression Gastroenterology Research and Practice |
| title | lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression |
| title_full | lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression |
| title_fullStr | lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression |
| title_full_unstemmed | lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression |
| title_short | lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression |
| title_sort | lncrna gau1 induces galnt8 overexpression and potentiates colorectal cancer progression |
| url | http://dx.doi.org/10.1155/2021/5960821 |
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