Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine quality

This study explores the potential of indigenous non-Saccharomyces yeasts isolated from Vitis vinifera L. grape skins to improve the quality of regional wines by enhancing their physicochemical and sensory characteristics. Five promising yeast strains were identified at different stages of fermentati...

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Main Authors: Sathivel Thivijan, Dayani Pavalakumar, Chathuri J. Gunathunga, Lanka J. S. Undugoda, Pathmalal M. Manage, Ruwani N. Nugara, Pasan C. Bandara, Kasun M. Thambugala, Fahad Al-Asmari, Itthayakorn Promputtha
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Cellular and Infection Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2024.1495177/full
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author Sathivel Thivijan
Sathivel Thivijan
Sathivel Thivijan
Dayani Pavalakumar
Dayani Pavalakumar
Chathuri J. Gunathunga
Chathuri J. Gunathunga
Lanka J. S. Undugoda
Pathmalal M. Manage
Ruwani N. Nugara
Pasan C. Bandara
Kasun M. Thambugala
Kasun M. Thambugala
Kasun M. Thambugala
Fahad Al-Asmari
Itthayakorn Promputtha
author_facet Sathivel Thivijan
Sathivel Thivijan
Sathivel Thivijan
Dayani Pavalakumar
Dayani Pavalakumar
Chathuri J. Gunathunga
Chathuri J. Gunathunga
Lanka J. S. Undugoda
Pathmalal M. Manage
Ruwani N. Nugara
Pasan C. Bandara
Kasun M. Thambugala
Kasun M. Thambugala
Kasun M. Thambugala
Fahad Al-Asmari
Itthayakorn Promputtha
author_sort Sathivel Thivijan
collection DOAJ
description This study explores the potential of indigenous non-Saccharomyces yeasts isolated from Vitis vinifera L. grape skins to improve the quality of regional wines by enhancing their physicochemical and sensory characteristics. Five promising yeast strains were identified at different stages of fermentation: Hanseniaspora opuntiae (J1Y-T1), H. guilliermondii (Y5P-T5), H. uvarum (JF3-T1N), Pichia kudriavzevii (Y8P-T8), and Starmerella bacillaris (WMP4-T4). Among these, H. uvarum and S. bacillaris were particularly noteworthy due to their superior alcohol production, achieving levels of 8.16 ± 0.05% and 8.04 ± 0.04% (v/v), respectively, and demonstrating higher alcohol tolerance even in later fermentation stages. Hanseniaspora uvarum also showed exceptional resilience, with a half-life of 3.34 ± 0.03 days and a Km value of 1.0200 ± 0.0100 mol L⁻¹, achieving the highest biomass even in the later stages of fermentation. High-Performance Liquid Chromatography analysis revealed that while tartaric acid levels remained constant, malic acid content decreased, and acetic acid was produced by all strains. Solid-Phase Microextraction-Gas Chromatography Mass Spectrometry identified ethyl acetate as the dominant volatile compound, with H. uvarum producing the highest concentration (43.411 ± 1.602%), contributing to a fruitier aroma and flavor. The combined attributes of H. uvarum higher alcohol content, enhanced fruity notes, improved clarity, lower acetic acid (0.52 ± 0.03 g L⁻¹), and significant residual sugar (162.37 ± 2.48 g L⁻¹) make it a promising candidate for improving the overall quality of regional wines. Incorporating H. uvarum into mixed starter cultures with specific Saccharomyces strains could further optimize the wine fermentation process.
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publisher Frontiers Media S.A.
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series Frontiers in Cellular and Infection Microbiology
spelling doaj-art-df0125fea13d44dcacc40b4189ef14d12024-12-06T06:50:44ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-12-011410.3389/fcimb.2024.14951771495177Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine qualitySathivel Thivijan0Sathivel Thivijan1Sathivel Thivijan2Dayani Pavalakumar3Dayani Pavalakumar4Chathuri J. Gunathunga5Chathuri J. Gunathunga6Lanka J. S. Undugoda7Pathmalal M. Manage8Ruwani N. Nugara9Pasan C. Bandara10Kasun M. Thambugala11Kasun M. Thambugala12Kasun M. Thambugala13Fahad Al-Asmari14Itthayakorn Promputtha15Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, ThailandDepartment of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Homagama, Sri LankaFaculty of Graduate Studies, University of Sri Jayewardenepura, Nugegoda, Sri LankaDepartment of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Homagama, Sri LankaFaculty of Graduate Studies, University of Sri Jayewardenepura, Nugegoda, Sri LankaDepartment of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Homagama, Sri LankaFaculty of Graduate Studies, University of Sri Jayewardenepura, Nugegoda, Sri LankaDepartment of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Homagama, Sri LankaCentre for Water Quality and Algae Research, Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri LankaDepartment of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Homagama, Sri LankaDepartment of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Homagama, Sri LankaGenetics and Molecular Biology Unit, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri LankaCenter for Biotechnology, Department of Zoology, University of Sri Jayewardenepura, Nugegoda, Sri LankaCenter for Plant Materials and Herbal Products Research, University of Sri Jayewardenepura, Nugegoda, Sri LankaDepartment of Food and Nutrition Sciences, College of Agricultural and Food Sciences, King Faisal University, Al Ahsa, Saudi ArabiaDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, ThailandThis study explores the potential of indigenous non-Saccharomyces yeasts isolated from Vitis vinifera L. grape skins to improve the quality of regional wines by enhancing their physicochemical and sensory characteristics. Five promising yeast strains were identified at different stages of fermentation: Hanseniaspora opuntiae (J1Y-T1), H. guilliermondii (Y5P-T5), H. uvarum (JF3-T1N), Pichia kudriavzevii (Y8P-T8), and Starmerella bacillaris (WMP4-T4). Among these, H. uvarum and S. bacillaris were particularly noteworthy due to their superior alcohol production, achieving levels of 8.16 ± 0.05% and 8.04 ± 0.04% (v/v), respectively, and demonstrating higher alcohol tolerance even in later fermentation stages. Hanseniaspora uvarum also showed exceptional resilience, with a half-life of 3.34 ± 0.03 days and a Km value of 1.0200 ± 0.0100 mol L⁻¹, achieving the highest biomass even in the later stages of fermentation. High-Performance Liquid Chromatography analysis revealed that while tartaric acid levels remained constant, malic acid content decreased, and acetic acid was produced by all strains. Solid-Phase Microextraction-Gas Chromatography Mass Spectrometry identified ethyl acetate as the dominant volatile compound, with H. uvarum producing the highest concentration (43.411 ± 1.602%), contributing to a fruitier aroma and flavor. The combined attributes of H. uvarum higher alcohol content, enhanced fruity notes, improved clarity, lower acetic acid (0.52 ± 0.03 g L⁻¹), and significant residual sugar (162.37 ± 2.48 g L⁻¹) make it a promising candidate for improving the overall quality of regional wines. Incorporating H. uvarum into mixed starter cultures with specific Saccharomyces strains could further optimize the wine fermentation process.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1495177/fullfermentation kineticsHanseniaspora uvarumHPLCsensory attributesVitis vinifera
spellingShingle Sathivel Thivijan
Sathivel Thivijan
Sathivel Thivijan
Dayani Pavalakumar
Dayani Pavalakumar
Chathuri J. Gunathunga
Chathuri J. Gunathunga
Lanka J. S. Undugoda
Pathmalal M. Manage
Ruwani N. Nugara
Pasan C. Bandara
Kasun M. Thambugala
Kasun M. Thambugala
Kasun M. Thambugala
Fahad Al-Asmari
Itthayakorn Promputtha
Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine quality
Frontiers in Cellular and Infection Microbiology
fermentation kinetics
Hanseniaspora uvarum
HPLC
sensory attributes
Vitis vinifera
title Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine quality
title_full Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine quality
title_fullStr Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine quality
title_full_unstemmed Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine quality
title_short Influence of indigenous non-Saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation: a promising approach to enhancing wine quality
title_sort influence of indigenous non saccharomyces yeast strains on the physicochemical and sensory properties of wine fermentation a promising approach to enhancing wine quality
topic fermentation kinetics
Hanseniaspora uvarum
HPLC
sensory attributes
Vitis vinifera
url https://www.frontiersin.org/articles/10.3389/fcimb.2024.1495177/full
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