PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes
Objective This study was conducted to reveal the role of nuclear poly(A) binding protein 1 (PABPN1) in the proliferation of preadipocytes, and to reveal the relationship between PABPN1 and cAMP response element (CRE)-binding protein (CREB) in the regulation of preadipocyte proliferation. Methods Vec...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2025-01-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0072.pdf |
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| author | Xiao-Han Zhang Jia-Xin Li Xiao-Xu Wu Qian Zhang Ming Tian Si-Qi Yang Di Liu Xiu-Qin Yang |
| author_facet | Xiao-Han Zhang Jia-Xin Li Xiao-Xu Wu Qian Zhang Ming Tian Si-Qi Yang Di Liu Xiu-Qin Yang |
| author_sort | Xiao-Han Zhang |
| collection | DOAJ |
| description | Objective This study was conducted to reveal the role of nuclear poly(A) binding protein 1 (PABPN1) in the proliferation of preadipocytes, and to reveal the relationship between PABPN1 and cAMP response element (CRE)-binding protein (CREB) in the regulation of preadipocyte proliferation. Methods Vectors overexpressing and siRNAs against PABPN1/CREB were transiently transfected into both porcine preadipocytes and mouse 3T3-L1 cells. Preadipocyte proliferation was measured with cell counting kit-8, 5-ethynyl-2′-deoxyuridine, real-time quantitative polymerase chain reaction, Western blotting, and flow cytometry analyses. Additionally, the transcriptional regulation of CREB on PABPN1 were analyzed with dual-luciferase reporter gene and electrophoretic mobility shift assay. Results Overexpression of PABPN1 inhibits, and knockdown of PABPN1 promotes, the proliferation of both porcine preadipocytes and 3T3-L1 cell lines. PABPN1 overexpression increased, while knockdown decreased, the cell population in the G0/G1 phase. These indicates that PABPN1 repressed preadipocyte proliferation by inhibiting cell cycle progress. Additionally, it was revealed that CREB regulated the expression of PABPN1 through binding to the promoter and that CREB inhibited preadipocyte proliferation by repressed cell cycle progress. Furthermore, we showed that PABPN1 functions as a downstream gene of CREB to regulate the proliferation of preadipocytes. Conclusion PABPN1 inhibits preadipocyte proliferation by suppressing the cell cycle. We also found that CREB could promote PABPN1 expression by binding to a motif in the promoter. Further analysis confirmed that PABPN1 functions as a downstream gene of CREB to regulate the proliferation of preadipocytes. These results suggest that the CREB/PABPN1 axis plays a role in the regulation of preadipocyte proliferation, which will contribute to further revealing the mechanism of fat accumulation. |
| format | Article |
| id | doaj-art-dee5b46cdaa444d4b5d4a73da4bbd954 |
| institution | Kabale University |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-dee5b46cdaa444d4b5d4a73da4bbd9542025-01-03T00:24:05ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-01-01381415310.5713/ab.24.007225327PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytesXiao-Han Zhang0Jia-Xin Li1Xiao-Xu Wu2Qian Zhang3Ming Tian4Si-Qi Yang5Di Liu6Xiu-Qin Yang7 College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaObjective This study was conducted to reveal the role of nuclear poly(A) binding protein 1 (PABPN1) in the proliferation of preadipocytes, and to reveal the relationship between PABPN1 and cAMP response element (CRE)-binding protein (CREB) in the regulation of preadipocyte proliferation. Methods Vectors overexpressing and siRNAs against PABPN1/CREB were transiently transfected into both porcine preadipocytes and mouse 3T3-L1 cells. Preadipocyte proliferation was measured with cell counting kit-8, 5-ethynyl-2′-deoxyuridine, real-time quantitative polymerase chain reaction, Western blotting, and flow cytometry analyses. Additionally, the transcriptional regulation of CREB on PABPN1 were analyzed with dual-luciferase reporter gene and electrophoretic mobility shift assay. Results Overexpression of PABPN1 inhibits, and knockdown of PABPN1 promotes, the proliferation of both porcine preadipocytes and 3T3-L1 cell lines. PABPN1 overexpression increased, while knockdown decreased, the cell population in the G0/G1 phase. These indicates that PABPN1 repressed preadipocyte proliferation by inhibiting cell cycle progress. Additionally, it was revealed that CREB regulated the expression of PABPN1 through binding to the promoter and that CREB inhibited preadipocyte proliferation by repressed cell cycle progress. Furthermore, we showed that PABPN1 functions as a downstream gene of CREB to regulate the proliferation of preadipocytes. Conclusion PABPN1 inhibits preadipocyte proliferation by suppressing the cell cycle. We also found that CREB could promote PABPN1 expression by binding to a motif in the promoter. Further analysis confirmed that PABPN1 functions as a downstream gene of CREB to regulate the proliferation of preadipocytes. These results suggest that the CREB/PABPN1 axis plays a role in the regulation of preadipocyte proliferation, which will contribute to further revealing the mechanism of fat accumulation.http://www.animbiosci.org/upload/pdf/ab-24-0072.pdfcrebpabpn1preadipocyteproliferationregulatory axis |
| spellingShingle | Xiao-Han Zhang Jia-Xin Li Xiao-Xu Wu Qian Zhang Ming Tian Si-Qi Yang Di Liu Xiu-Qin Yang PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes Animal Bioscience creb pabpn1 preadipocyte proliferation regulatory axis |
| title | PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes |
| title_full | PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes |
| title_fullStr | PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes |
| title_full_unstemmed | PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes |
| title_short | PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes |
| title_sort | pabpn1 functions as a downstream gene of creb to inhibit the proliferation of preadipocytes |
| topic | creb pabpn1 preadipocyte proliferation regulatory axis |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0072.pdf |
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