Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model
Abstract Acute kidney injury (AKI) has been reported to occur in 30–70% of asphyxiated neonates. Hydrogen (H2) gas became a major research focus in neonatal medicine after the identification of its robust antioxidative properties. However, the ability of H2 gas to ameliorate AKI is unknown. We exami...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-85231-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544853542928384 |
|---|---|
| author | Takuma Iwaki Shinji Nakamura Takayuki Wakabayashi Yasuhiro Nakao Yinmon Htun Toui Tsuchiya Tsutomu Mitsuie Kosuke Koyano Aya Morimoto Noriko Fuke Takayuki Yokota Sonoko Kondo Yukihiko Konishi Takanori Miki Masaki Ueno Takashi Iwase Takashi Kusaka |
| author_facet | Takuma Iwaki Shinji Nakamura Takayuki Wakabayashi Yasuhiro Nakao Yinmon Htun Toui Tsuchiya Tsutomu Mitsuie Kosuke Koyano Aya Morimoto Noriko Fuke Takayuki Yokota Sonoko Kondo Yukihiko Konishi Takanori Miki Masaki Ueno Takashi Iwase Takashi Kusaka |
| author_sort | Takuma Iwaki |
| collection | DOAJ |
| description | Abstract Acute kidney injury (AKI) has been reported to occur in 30–70% of asphyxiated neonates. Hydrogen (H2) gas became a major research focus in neonatal medicine after the identification of its robust antioxidative properties. However, the ability of H2 gas to ameliorate AKI is unknown. We examined histopathological injuries in the piglet renal cortex on day 5 after a hypoxic-ischemic (HI) insult and if H2 gas can alleviate kidney injuries. Twenty piglets were divided into three groups: no insult (Control, n = 6), HI insult alone (HI, n = 8), and HI insult with H2 gas ventilation (HI-H2, 2.1–2.7% for 24 h, n = 6). The total glomerular cell count was significantly higher in the HI group than in the other groups, with no difference between the HI-H2 and control groups. Proximal tubular lumen narrowing was significantly increased in the HI group versus control, but not in the HI-H2 group. In this piglet model, glomerular enlargement with an increase in glomerular cell number due to tubular lumen narrowing was observed on day 5 after HI insult. H2 gas effectively suppressed this glomerular cell increase and tubular lumen narrowing. |
| format | Article |
| id | doaj-art-dec0c9047dcf437391400d987425ce67 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-dec0c9047dcf437391400d987425ce672025-01-12T12:16:18ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-85231-8Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet modelTakuma Iwaki0Shinji Nakamura1Takayuki Wakabayashi2Yasuhiro Nakao3Yinmon Htun4Toui Tsuchiya5Tsutomu Mitsuie6Kosuke Koyano7Aya Morimoto8Noriko Fuke9Takayuki Yokota10Sonoko Kondo11Yukihiko Konishi12Takanori Miki13Masaki Ueno14Takashi Iwase15Takashi Kusaka16Department of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityMedical Engineering Equipment Management Center, Kagawa University Hospital, Kagawa UniversityMaternal Perinatal Center, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Anatomy and Neurobiology, Faculty of Medicine, Kagawa UniversityDepartment of Pathology and Host Defense, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityDepartment of Pediatrics, Faculty of Medicine, Kagawa UniversityAbstract Acute kidney injury (AKI) has been reported to occur in 30–70% of asphyxiated neonates. Hydrogen (H2) gas became a major research focus in neonatal medicine after the identification of its robust antioxidative properties. However, the ability of H2 gas to ameliorate AKI is unknown. We examined histopathological injuries in the piglet renal cortex on day 5 after a hypoxic-ischemic (HI) insult and if H2 gas can alleviate kidney injuries. Twenty piglets were divided into three groups: no insult (Control, n = 6), HI insult alone (HI, n = 8), and HI insult with H2 gas ventilation (HI-H2, 2.1–2.7% for 24 h, n = 6). The total glomerular cell count was significantly higher in the HI group than in the other groups, with no difference between the HI-H2 and control groups. Proximal tubular lumen narrowing was significantly increased in the HI group versus control, but not in the HI-H2 group. In this piglet model, glomerular enlargement with an increase in glomerular cell number due to tubular lumen narrowing was observed on day 5 after HI insult. H2 gas effectively suppressed this glomerular cell increase and tubular lumen narrowing.https://doi.org/10.1038/s41598-025-85231-8Acute kidney injuryAsphyxiaGlomerular enlargementHydrogen gasHypoxic-ischemiaPiglet |
| spellingShingle | Takuma Iwaki Shinji Nakamura Takayuki Wakabayashi Yasuhiro Nakao Yinmon Htun Toui Tsuchiya Tsutomu Mitsuie Kosuke Koyano Aya Morimoto Noriko Fuke Takayuki Yokota Sonoko Kondo Yukihiko Konishi Takanori Miki Masaki Ueno Takashi Iwase Takashi Kusaka Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model Scientific Reports Acute kidney injury Asphyxia Glomerular enlargement Hydrogen gas Hypoxic-ischemia Piglet |
| title | Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model |
| title_full | Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model |
| title_fullStr | Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model |
| title_full_unstemmed | Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model |
| title_short | Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model |
| title_sort | hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic ischemic insult in asphyxiated piglet model |
| topic | Acute kidney injury Asphyxia Glomerular enlargement Hydrogen gas Hypoxic-ischemia Piglet |
| url | https://doi.org/10.1038/s41598-025-85231-8 |
| work_keys_str_mv | AT takumaiwaki hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT shinjinakamura hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT takayukiwakabayashi hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT yasuhironakao hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT yinmonhtun hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT touitsuchiya hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT tsutomumitsuie hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT kosukekoyano hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT ayamorimoto hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT norikofuke hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT takayukiyokota hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT sonokokondo hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT yukihikokonishi hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT takanorimiki hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT masakiueno hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT takashiiwase hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel AT takashikusaka hydrogengasinhalationamelioratesglomerularenlargementafterhypoxicischemicinsultinasphyxiatedpigletmodel |