Low cost, portable, 3D printable tissue precision slicer
Slicing tissue samples into thin pieces is commonly used in histology analysis and more recently for organotypic culture when tissue samples are sliced alive. Currently available devices for slicing tissue samples are either designed for fixed tissue samples at low cryogenic temperatures (e.g., Cryo...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | HardwareX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468067224001056 |
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| author | Beatriz Martinez-Martin Isabella Lambros Lukas Nuesslein Yubing Sun |
| author_facet | Beatriz Martinez-Martin Isabella Lambros Lukas Nuesslein Yubing Sun |
| author_sort | Beatriz Martinez-Martin |
| collection | DOAJ |
| description | Slicing tissue samples into thin pieces is commonly used in histology analysis and more recently for organotypic culture when tissue samples are sliced alive. Currently available devices for slicing tissue samples are either designed for fixed tissue samples at low cryogenic temperatures (e.g., Cryostats), or bulky and expensive (e.g., vibratome), preventing them from routine lab usage. Here we report a cost-effective device designed to section live tissues for subsequent culture. This device consists of components crafted from 3D-printed Nylon-12- a material suitable for autoclaving to ensure sterility. Its small footprint enhances portability, allowing for convenient placement within a biosafety cabinet for an added layer of sterility assurance. Using human pluripotent stem cells derived brain organoids as an example, we demonstrated that the device both precisely and accurately makes slices. We further validate its suitability for long-term culture by extended tissue culture following slicing. Our results indicate that brain organoid slices are viable and show improved proliferation rate compared with unsliced organoids. |
| format | Article |
| id | doaj-art-de51a095902848f5a35b37afcbfc6f6e |
| institution | Kabale University |
| issn | 2468-0672 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj-art-de51a095902848f5a35b37afcbfc6f6e2024-12-16T05:36:44ZengElsevierHardwareX2468-06722024-12-0120e00611Low cost, portable, 3D printable tissue precision slicerBeatriz Martinez-Martin0Isabella Lambros1Lukas Nuesslein2Yubing Sun3Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst, MA 01003, United StatesDepartment of Mechanical and Industrial Engineering, University of Massachusetts Amherst, Amherst, MA 01003, United StatesDepartment of Mechanical and Industrial Engineering, University of Massachusetts Amherst, Amherst, MA 01003, United StatesMolecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst, MA 01003, United States; Department of Mechanical and Industrial Engineering, University of Massachusetts Amherst, Amherst, MA 01003, United States; Department of Biomedical Engineering, University of Massachusetts Amherst, Amherst, MA 01003, United States; Corresponding author at: Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst, MA 01003, United States.Slicing tissue samples into thin pieces is commonly used in histology analysis and more recently for organotypic culture when tissue samples are sliced alive. Currently available devices for slicing tissue samples are either designed for fixed tissue samples at low cryogenic temperatures (e.g., Cryostats), or bulky and expensive (e.g., vibratome), preventing them from routine lab usage. Here we report a cost-effective device designed to section live tissues for subsequent culture. This device consists of components crafted from 3D-printed Nylon-12- a material suitable for autoclaving to ensure sterility. Its small footprint enhances portability, allowing for convenient placement within a biosafety cabinet for an added layer of sterility assurance. Using human pluripotent stem cells derived brain organoids as an example, we demonstrated that the device both precisely and accurately makes slices. We further validate its suitability for long-term culture by extended tissue culture following slicing. Our results indicate that brain organoid slices are viable and show improved proliferation rate compared with unsliced organoids.http://www.sciencedirect.com/science/article/pii/S2468067224001056Tissue slicesMicrotomyOrganoidsLow-cost |
| spellingShingle | Beatriz Martinez-Martin Isabella Lambros Lukas Nuesslein Yubing Sun Low cost, portable, 3D printable tissue precision slicer HardwareX Tissue slices Microtomy Organoids Low-cost |
| title | Low cost, portable, 3D printable tissue precision slicer |
| title_full | Low cost, portable, 3D printable tissue precision slicer |
| title_fullStr | Low cost, portable, 3D printable tissue precision slicer |
| title_full_unstemmed | Low cost, portable, 3D printable tissue precision slicer |
| title_short | Low cost, portable, 3D printable tissue precision slicer |
| title_sort | low cost portable 3d printable tissue precision slicer |
| topic | Tissue slices Microtomy Organoids Low-cost |
| url | http://www.sciencedirect.com/science/article/pii/S2468067224001056 |
| work_keys_str_mv | AT beatrizmartinezmartin lowcostportable3dprintabletissueprecisionslicer AT isabellalambros lowcostportable3dprintabletissueprecisionslicer AT lukasnuesslein lowcostportable3dprintabletissueprecisionslicer AT yubingsun lowcostportable3dprintabletissueprecisionslicer |