Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i>
<i>Phytophthora capsici</i> is a devastating pathogen in horticultural crops, particularly affecting <i>Capsicum annuum</i> (pepper). The overuse of chemical fungicides has led to resistance development, necessitating alternative strategies. This study investigates the antago...
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MDPI AG
2024-11-01
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| author | José Luis Ávila-Oviedo Carlos Méndez-Inocencio María Dolores Rodríguez-Torres María Valentina Angoa-Pérez Mauricio Nahuam Chávez-Avilés Erika Karina Martínez-Mendoza Ernesto Oregel-Zamudio Edgar Villar-Luna |
| author_facet | José Luis Ávila-Oviedo Carlos Méndez-Inocencio María Dolores Rodríguez-Torres María Valentina Angoa-Pérez Mauricio Nahuam Chávez-Avilés Erika Karina Martínez-Mendoza Ernesto Oregel-Zamudio Edgar Villar-Luna |
| author_sort | José Luis Ávila-Oviedo |
| collection | DOAJ |
| description | <i>Phytophthora capsici</i> is a devastating pathogen in horticultural crops, particularly affecting <i>Capsicum annuum</i> (pepper). The overuse of chemical fungicides has led to resistance development, necessitating alternative strategies. This study investigates the antagonistic effects of four rhizobacterial isolates (<i>Bacillus</i> sp., <i>Pseudomonas putida</i>, <i>Bacillus subtilis</i>, <i>Bacillus amyloliquefaciens</i>) against <i>P. capsici</i>, focusing on the production of volatile organic compounds (VOCs). Using in vitro dual culture assays, we observed a significant inhibition of mycelial growth and sporangia production, especially by <i>B. subtilis</i> and <i>B. amyloliquefaciens</i>. The GC-MS/SPME-HS analysis identified key VOCs responsible for these antagonistic effects. Our findings demonstrate that specific rhizobacteria and their VOCs offer a promising biocontrol strategy, potentially reducing the reliance on chemical fungicides and contributing to sustainable agriculture. |
| format | Article |
| id | doaj-art-dde85ece17e94129bfcd4a5ed410680c |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-dde85ece17e94129bfcd4a5ed410680c2024-11-26T18:19:00ZengMDPI AGPlants2223-77472024-11-011322322410.3390/plants13223224Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i>José Luis Ávila-Oviedo0Carlos Méndez-Inocencio1María Dolores Rodríguez-Torres2María Valentina Angoa-Pérez3Mauricio Nahuam Chávez-Avilés4Erika Karina Martínez-Mendoza5Ernesto Oregel-Zamudio6Edgar Villar-Luna7Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Michoacán, Justo Sierra 28, Col. Centro, Jiquilpan 59510, Michoacán, MexicoInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Michoacán, Justo Sierra 28, Col. Centro, Jiquilpan 59510, Michoacán, MexicoInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Michoacán, Justo Sierra 28, Col. Centro, Jiquilpan 59510, Michoacán, MexicoInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Michoacán, Justo Sierra 28, Col. Centro, Jiquilpan 59510, Michoacán, MexicoLaboratorio de Bioquímica y Biología Molecular, División de Ingeniería Bioquímica, Tecnológico Nacional de México/ITS de Ciudad Hidalgo, Av. Ing. Carlos Rojas Gutiérrez No. 2120 Fracc. Valle de La Herradura C.P., Hidalgo 61100, Michoacán, MexicoInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Michoacán, Justo Sierra 28, Col. Centro, Jiquilpan 59510, Michoacán, MexicoInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Michoacán, Justo Sierra 28, Col. Centro, Jiquilpan 59510, Michoacán, MexicoInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Michoacán, Justo Sierra 28, Col. Centro, Jiquilpan 59510, Michoacán, Mexico<i>Phytophthora capsici</i> is a devastating pathogen in horticultural crops, particularly affecting <i>Capsicum annuum</i> (pepper). The overuse of chemical fungicides has led to resistance development, necessitating alternative strategies. This study investigates the antagonistic effects of four rhizobacterial isolates (<i>Bacillus</i> sp., <i>Pseudomonas putida</i>, <i>Bacillus subtilis</i>, <i>Bacillus amyloliquefaciens</i>) against <i>P. capsici</i>, focusing on the production of volatile organic compounds (VOCs). Using in vitro dual culture assays, we observed a significant inhibition of mycelial growth and sporangia production, especially by <i>B. subtilis</i> and <i>B. amyloliquefaciens</i>. The GC-MS/SPME-HS analysis identified key VOCs responsible for these antagonistic effects. Our findings demonstrate that specific rhizobacteria and their VOCs offer a promising biocontrol strategy, potentially reducing the reliance on chemical fungicides and contributing to sustainable agriculture.https://www.mdpi.com/2223-7747/13/22/3224biological controlfungicide alternativesmicrobial interactionsplant disease managementsustainable agriculture |
| spellingShingle | José Luis Ávila-Oviedo Carlos Méndez-Inocencio María Dolores Rodríguez-Torres María Valentina Angoa-Pérez Mauricio Nahuam Chávez-Avilés Erika Karina Martínez-Mendoza Ernesto Oregel-Zamudio Edgar Villar-Luna Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i> Plants biological control fungicide alternatives microbial interactions plant disease management sustainable agriculture |
| title | Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i> |
| title_full | Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i> |
| title_fullStr | Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i> |
| title_full_unstemmed | Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i> |
| title_short | Antagonistic Effects and Volatile Organic Compound Profiles of Rhizobacteria in the Biocontrol of <i>Phytophthora capsici</i> |
| title_sort | antagonistic effects and volatile organic compound profiles of rhizobacteria in the biocontrol of i phytophthora capsici i |
| topic | biological control fungicide alternatives microbial interactions plant disease management sustainable agriculture |
| url | https://www.mdpi.com/2223-7747/13/22/3224 |
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