d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activities
Abstract Amino acids play essential roles in various biological processes. In humans, most amino acids are present in the l-form; however, small amounts of d-amino acids also exist and have significant physiological roles, highlighting the importance of dietary intake from foods or drinks. In this s...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-10379-2 |
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| author | Mayu Onozato Tomoya Takaura Wataru Shinohara Takehiro Tsukada Tatsuya Sakamoto Kenji Okoshi Takeshi Fukushima |
| author_facet | Mayu Onozato Tomoya Takaura Wataru Shinohara Takehiro Tsukada Tatsuya Sakamoto Kenji Okoshi Takeshi Fukushima |
| author_sort | Mayu Onozato |
| collection | DOAJ |
| description | Abstract Amino acids play essential roles in various biological processes. In humans, most amino acids are present in the l-form; however, small amounts of d-amino acids also exist and have significant physiological roles, highlighting the importance of dietary intake from foods or drinks. In this study, we investigated the amino acid composition of the geoduck clam Panopea japonica, emphasizing its remarkably high d-alanine (d-Ala) content in the siphon tissue. The d-Ala content (6.99–14.2 mmol/100 g-wet) amounted to 91–94% of the total Ala, far exceeding that of other bivalves such as Tresus keenae (74%). Enzyme assays revealed alanine racemase and d-amino acid oxidase activities, suggesting active d-Ala biosynthesis and metabolism. The high concentrations of d-Ala enhance its value as a delicacy owing to its unique sweetness. This study provides new insights into the biosynthesis and metabolic characteristics of d-Ala in bivalves, highlighting its physiological and food ingredient significance. |
| format | Article |
| id | doaj-art-ddbe0aaff7f94ff29e4d63e6830fc8d6 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ddbe0aaff7f94ff29e4d63e6830fc8d62025-08-20T04:02:45ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-10379-2d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activitiesMayu Onozato0Tomoya Takaura1Wataru Shinohara2Takehiro Tsukada3Tatsuya Sakamoto4Kenji Okoshi5Takeshi Fukushima6Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho UniversityDepartment of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho UniversityChiba Municipal Chiba High SchoolDepartment of Biomolecular Science, Faculty of Science, Toho UniversityDepartment of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho UniversityDepartment of Environmental Science, Faculty of Science, Toho UniversityDepartment of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho UniversityAbstract Amino acids play essential roles in various biological processes. In humans, most amino acids are present in the l-form; however, small amounts of d-amino acids also exist and have significant physiological roles, highlighting the importance of dietary intake from foods or drinks. In this study, we investigated the amino acid composition of the geoduck clam Panopea japonica, emphasizing its remarkably high d-alanine (d-Ala) content in the siphon tissue. The d-Ala content (6.99–14.2 mmol/100 g-wet) amounted to 91–94% of the total Ala, far exceeding that of other bivalves such as Tresus keenae (74%). Enzyme assays revealed alanine racemase and d-amino acid oxidase activities, suggesting active d-Ala biosynthesis and metabolism. The high concentrations of d-Ala enhance its value as a delicacy owing to its unique sweetness. This study provides new insights into the biosynthesis and metabolic characteristics of d-Ala in bivalves, highlighting its physiological and food ingredient significance.https://doi.org/10.1038/s41598-025-10379-2d-AlanineBivalveAmino acidSiphonAlanine racemased-amino acid oxidase |
| spellingShingle | Mayu Onozato Tomoya Takaura Wataru Shinohara Takehiro Tsukada Tatsuya Sakamoto Kenji Okoshi Takeshi Fukushima d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activities Scientific Reports d-Alanine Bivalve Amino acid Siphon Alanine racemase d-amino acid oxidase |
| title | d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activities |
| title_full | d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activities |
| title_fullStr | d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activities |
| title_full_unstemmed | d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activities |
| title_short | d-Alanine content in the marine edible bivalve Panopea japonica and evaluation of its associated enzyme activities |
| title_sort | d alanine content in the marine edible bivalve panopea japonica and evaluation of its associated enzyme activities |
| topic | d-Alanine Bivalve Amino acid Siphon Alanine racemase d-amino acid oxidase |
| url | https://doi.org/10.1038/s41598-025-10379-2 |
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