Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms
Background: It is well known that cryopreservation procedure may result in negative impact on spermatozoa function throughout relative overproduction of reactive oxygen species (ROS). However, ROS overproduction can be corrected by antioxidants. Objective: To find out the effect of vitamin A on...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Al-Nahrain University/College of medicine
2023-06-01
|
| Series: | The Iraqi Journal of Medical Sciences |
| Online Access: | https://www.iraqijms.net/index.php?do=view&type=article&id=856 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849220344071585792 |
|---|---|
| author | Samir Al-Anbari Mustafa Ibraheem Thaer Farhan |
| author_facet | Samir Al-Anbari Mustafa Ibraheem Thaer Farhan |
| author_sort | Samir Al-Anbari |
| collection | DOAJ |
| description |
Background: It is well known that cryopreservation procedure may result in negative impact on spermatozoa function throughout relative overproduction of reactive oxygen species (ROS). However, ROS overproduction can be corrected by antioxidants.
Objective: To find out the effect of vitamin A on cryopreserved spermatozoa throughout measuring their motility rate and DNA fragmentation and compare it with vitamin E effect.
Methods: Forty seminal fluid specimens were individually collected from forty healthy, non-drug and non-alcohol consumers, normozoospermic males in Um Al-Baneen Center for Infertility Management and In Vitro Fertilization in Baghdad. To facilitate comparing between used vitamins and experimenting each one alone, those specimens were distributed into 4 groups of 10 specimens each. Following deriving their own controls without any vitamin treatment, specimens of these groups, in general, were treated with two concentrations of vitamin A (20 µg/dl and 30 µg/dl), and two concentrations of vitamin E (10 µmol/l and 20 µmol/l). Then, each specimen was incubated for 1 hour before being cryopreserved in liquid nitrogen for 14 days. Motility percentage and DNA fragmentation were assessed following cryopreservation and thawing of spermatozoa.
Results: Results revealed that there were significant statistically differences in post-thawing motility and DNA fragmentation means between specimens treated with vitamin A and their relevant control; between specimens treated with vitamin E and their relevant control; and between specimens treated with vitamin E and those treated with vitamin A among all groups of the study.
Conclusion: These results lead to the conclusion that both of vitamin A and E play an important role in improving and protecting sperm motility and DNA integrity following cryopreservation, and vitamin E is more effective than vitamin A.
Keywords: Seminal fluid analysis, vitamin A, vitamin E, reactive oxygen species, sperm cryopreservation, sperm DNA fragmentation, sperm motility assessment
Citation: Al-Anbari SA, Ibraheem MM, Farhan TM. Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms. Iraqi JMS. 2023; 21(1): 43-62. doi: 10.22578/IJMS.21.1.5 |
| format | Article |
| id | doaj-art-dd7b8a34863b471b90c8deb6179f0a7b |
| institution | Kabale University |
| issn | 1681-6579 2224-4719 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Al-Nahrain University/College of medicine |
| record_format | Article |
| series | The Iraqi Journal of Medical Sciences |
| spelling | doaj-art-dd7b8a34863b471b90c8deb6179f0a7b2024-12-13T20:02:34ZengAl-Nahrain University/College of medicineThe Iraqi Journal of Medical Sciences1681-65792224-47192023-06-01211436210.22578/IJMS.21.1.5Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved SpermsSamir Al-AnbariMustafa IbraheemThaer Farhan Background: It is well known that cryopreservation procedure may result in negative impact on spermatozoa function throughout relative overproduction of reactive oxygen species (ROS). However, ROS overproduction can be corrected by antioxidants. Objective: To find out the effect of vitamin A on cryopreserved spermatozoa throughout measuring their motility rate and DNA fragmentation and compare it with vitamin E effect. Methods: Forty seminal fluid specimens were individually collected from forty healthy, non-drug and non-alcohol consumers, normozoospermic males in Um Al-Baneen Center for Infertility Management and In Vitro Fertilization in Baghdad. To facilitate comparing between used vitamins and experimenting each one alone, those specimens were distributed into 4 groups of 10 specimens each. Following deriving their own controls without any vitamin treatment, specimens of these groups, in general, were treated with two concentrations of vitamin A (20 µg/dl and 30 µg/dl), and two concentrations of vitamin E (10 µmol/l and 20 µmol/l). Then, each specimen was incubated for 1 hour before being cryopreserved in liquid nitrogen for 14 days. Motility percentage and DNA fragmentation were assessed following cryopreservation and thawing of spermatozoa. Results: Results revealed that there were significant statistically differences in post-thawing motility and DNA fragmentation means between specimens treated with vitamin A and their relevant control; between specimens treated with vitamin E and their relevant control; and between specimens treated with vitamin E and those treated with vitamin A among all groups of the study. Conclusion: These results lead to the conclusion that both of vitamin A and E play an important role in improving and protecting sperm motility and DNA integrity following cryopreservation, and vitamin E is more effective than vitamin A. Keywords: Seminal fluid analysis, vitamin A, vitamin E, reactive oxygen species, sperm cryopreservation, sperm DNA fragmentation, sperm motility assessment Citation: Al-Anbari SA, Ibraheem MM, Farhan TM. Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms. Iraqi JMS. 2023; 21(1): 43-62. doi: 10.22578/IJMS.21.1.5https://www.iraqijms.net/index.php?do=view&type=article&id=856 |
| spellingShingle | Samir Al-Anbari Mustafa Ibraheem Thaer Farhan Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms The Iraqi Journal of Medical Sciences |
| title | Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms |
| title_full | Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms |
| title_fullStr | Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms |
| title_full_unstemmed | Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms |
| title_short | Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms |
| title_sort | assessment of vitamin a versus vitamin e effect on motility and dna integrity of human cryopreserved sperms |
| url | https://www.iraqijms.net/index.php?do=view&type=article&id=856 |
| work_keys_str_mv | AT samiralanbari assessmentofvitaminaversusvitamineeffectonmotilityanddnaintegrityofhumancryopreservedsperms AT mustafaibraheem assessmentofvitaminaversusvitamineeffectonmotilityanddnaintegrityofhumancryopreservedsperms AT thaerfarhan assessmentofvitaminaversusvitamineeffectonmotilityanddnaintegrityofhumancryopreservedsperms |