Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pH
Hyaluronic acid (HA) is an acidic mucopolysaccharide of animal origin composed of repeating disaccharide units of N-acetylglucosamine and glucuronic acid. Due to its excellent biocompatibility, biodegradability, and selective affinity for CD44 receptors on cell surfaces, HA is widely employed as a d...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/29/24/5971 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846103446406561792 |
|---|---|
| author | Jun Luo Hui Huang Junfeng Jiang Wenyu Zheng Peng Chen Hongjin Bai |
| author_facet | Jun Luo Hui Huang Junfeng Jiang Wenyu Zheng Peng Chen Hongjin Bai |
| author_sort | Jun Luo |
| collection | DOAJ |
| description | Hyaluronic acid (HA) is an acidic mucopolysaccharide of animal origin composed of repeating disaccharide units of N-acetylglucosamine and glucuronic acid. Due to its excellent biocompatibility, biodegradability, and selective affinity for CD44 receptors on cell surfaces, HA is widely employed as a drug carrier. In our study, we aimed to target subcellular bacteria by grafting cystamine onto HA scaffolds through an amide reaction, producing a linker responsive to H<sub>2</sub>S and pH changes. Subsequently, hydrophobic dodecylamine was attached to HA, forming amphiphilic molecules. These amphiphilic entities can self-assemble into nanomicelles in an aqueous solution, thereby encapsulating the antibacterial agent triclosan (TCS). The resulting HA-based system (HASS-TCS) can be internalized via CD44-mediated endocytosis, releasing substantial amounts of streptomycin and TCS in H<sub>2</sub>S-rich and acidic environments. Additionally, HASS-TCS has demonstrated effectiveness in eradicating biofilms and addressing intracellular infections caused by <i>Salmonella</i>. This study underscores a novel pH-sensitive hyaluronic acid-based drug delivery system with significant potential for the effective treatment of intracellular infections. |
| format | Article |
| id | doaj-art-dcfd41c4846346d5b4fa4ba777a9dbe7 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-dcfd41c4846346d5b4fa4ba777a9dbe72024-12-27T14:42:48ZengMDPI AGMolecules1420-30492024-12-012924597110.3390/molecules29245971Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pHJun Luo0Hui Huang1Junfeng Jiang2Wenyu Zheng3Peng Chen4Hongjin Bai5Engineering Laboratory of Chemical Resources Utilization in South Xinjiang, Tarim University, Alar 843300, ChinaEngineering Laboratory of Chemical Resources Utilization in South Xinjiang, Tarim University, Alar 843300, ChinaEngineering Laboratory of Chemical Resources Utilization in South Xinjiang, Tarim University, Alar 843300, ChinaEngineering Laboratory of Chemical Resources Utilization in South Xinjiang, Tarim University, Alar 843300, ChinaEngineering Laboratory of Chemical Resources Utilization in South Xinjiang, Tarim University, Alar 843300, ChinaEngineering Laboratory of Chemical Resources Utilization in South Xinjiang, Tarim University, Alar 843300, ChinaHyaluronic acid (HA) is an acidic mucopolysaccharide of animal origin composed of repeating disaccharide units of N-acetylglucosamine and glucuronic acid. Due to its excellent biocompatibility, biodegradability, and selective affinity for CD44 receptors on cell surfaces, HA is widely employed as a drug carrier. In our study, we aimed to target subcellular bacteria by grafting cystamine onto HA scaffolds through an amide reaction, producing a linker responsive to H<sub>2</sub>S and pH changes. Subsequently, hydrophobic dodecylamine was attached to HA, forming amphiphilic molecules. These amphiphilic entities can self-assemble into nanomicelles in an aqueous solution, thereby encapsulating the antibacterial agent triclosan (TCS). The resulting HA-based system (HASS-TCS) can be internalized via CD44-mediated endocytosis, releasing substantial amounts of streptomycin and TCS in H<sub>2</sub>S-rich and acidic environments. Additionally, HASS-TCS has demonstrated effectiveness in eradicating biofilms and addressing intracellular infections caused by <i>Salmonella</i>. This study underscores a novel pH-sensitive hyaluronic acid-based drug delivery system with significant potential for the effective treatment of intracellular infections.https://www.mdpi.com/1420-3049/29/24/5971hyaluronic acidpH and H<sub>2</sub>S dual-responsiveintracellular bacteriabiofilm |
| spellingShingle | Jun Luo Hui Huang Junfeng Jiang Wenyu Zheng Peng Chen Hongjin Bai Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pH Molecules hyaluronic acid pH and H<sub>2</sub>S dual-responsive intracellular bacteria biofilm |
| title | Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pH |
| title_full | Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pH |
| title_fullStr | Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pH |
| title_full_unstemmed | Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pH |
| title_short | Clearance of Intracellular Pathogens with Hyaluronic Acid Nanomicelles Responsive to H<sub>2</sub>S and pH |
| title_sort | clearance of intracellular pathogens with hyaluronic acid nanomicelles responsive to h sub 2 sub s and ph |
| topic | hyaluronic acid pH and H<sub>2</sub>S dual-responsive intracellular bacteria biofilm |
| url | https://www.mdpi.com/1420-3049/29/24/5971 |
| work_keys_str_mv | AT junluo clearanceofintracellularpathogenswithhyaluronicacidnanomicellesresponsivetohsub2subsandph AT huihuang clearanceofintracellularpathogenswithhyaluronicacidnanomicellesresponsivetohsub2subsandph AT junfengjiang clearanceofintracellularpathogenswithhyaluronicacidnanomicellesresponsivetohsub2subsandph AT wenyuzheng clearanceofintracellularpathogenswithhyaluronicacidnanomicellesresponsivetohsub2subsandph AT pengchen clearanceofintracellularpathogenswithhyaluronicacidnanomicellesresponsivetohsub2subsandph AT hongjinbai clearanceofintracellularpathogenswithhyaluronicacidnanomicellesresponsivetohsub2subsandph |