Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i>
Microbial enhanced oil recovery (MEOR) is a promising technology for oil field extraction. This study investigated a co-culture system of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> to increase MEOR efficacy. We analyzed bacterial growth, biosurfactant producti...
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2024-11-01
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| author | Dingyu Kang Hai Lin Qiang Li Nan Su Changkun Cheng Yijing Luo Zhongzhi Zhang Zhiyong Zhang |
| author_facet | Dingyu Kang Hai Lin Qiang Li Nan Su Changkun Cheng Yijing Luo Zhongzhi Zhang Zhiyong Zhang |
| author_sort | Dingyu Kang |
| collection | DOAJ |
| description | Microbial enhanced oil recovery (MEOR) is a promising technology for oil field extraction. This study investigated a co-culture system of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> to increase MEOR efficacy. We analyzed bacterial growth, biosurfactant production, and crude oil emulsified performance under different inoculation ratios. Compared to single cultures, the co-culture system showed superior growth and functional expression, with an optimal inoculation ratio of 1:1. Quantitative assessments of the cell numbers and biosurfactant production during the co-culture revealed that rapid <i>B. subtilis</i> proliferation in early stages significantly stimulated <i>P. aeruginosa</i> growth. This interaction increased cell density and rhamnolipid production by 208.05% and 216.25%, respectively. The microscopic etching model displacement results demonstrated enhanced emulsification and mobilization of crude oil by the co-culture system, resulting in 94.48% recovery. A successful field application in a block-scale reservoir increased cumulative oil production by 3.25 × 10<sup>3</sup> t. An analysis of microbial community structure and function in different phases revealed that after co-culture system injection, <i>Pseudomonas</i> became the dominant genus in the reservoir community, with an average abundance of 24.80%. Additionally, the abundance of biosurfactant-producing and hydrocarbon-degrading bacteria increased significantly. This research and the application of the <i>P. aeruginosa</i> and <i>B. subtilis</i> co-culture system provide novel insights and strategies for MEOR. |
| format | Article |
| id | doaj-art-dce6aedcc5044888ba6976ce72ecdb3f |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-dce6aedcc5044888ba6976ce72ecdb3f2024-11-26T18:15:01ZengMDPI AGMicroorganisms2076-26072024-11-011211234310.3390/microorganisms12112343Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i>Dingyu Kang0Hai Lin1Qiang Li2Nan Su3Changkun Cheng4Yijing Luo5Zhongzhi Zhang6Zhiyong Zhang7State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, ChinaState Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, ChinaDrilling and Production Technology Research Institute of CNPC Qinghai Oilfield, Dunhuang 736202, ChinaDrilling and Production Technology Research Institute of CNPC Qinghai Oilfield, Dunhuang 736202, ChinaDrilling and Production Technology Research Institute of CNPC Qinghai Oilfield, Dunhuang 736202, ChinaState Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, ChinaState Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, ChinaState Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, ChinaMicrobial enhanced oil recovery (MEOR) is a promising technology for oil field extraction. This study investigated a co-culture system of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> to increase MEOR efficacy. We analyzed bacterial growth, biosurfactant production, and crude oil emulsified performance under different inoculation ratios. Compared to single cultures, the co-culture system showed superior growth and functional expression, with an optimal inoculation ratio of 1:1. Quantitative assessments of the cell numbers and biosurfactant production during the co-culture revealed that rapid <i>B. subtilis</i> proliferation in early stages significantly stimulated <i>P. aeruginosa</i> growth. This interaction increased cell density and rhamnolipid production by 208.05% and 216.25%, respectively. The microscopic etching model displacement results demonstrated enhanced emulsification and mobilization of crude oil by the co-culture system, resulting in 94.48% recovery. A successful field application in a block-scale reservoir increased cumulative oil production by 3.25 × 10<sup>3</sup> t. An analysis of microbial community structure and function in different phases revealed that after co-culture system injection, <i>Pseudomonas</i> became the dominant genus in the reservoir community, with an average abundance of 24.80%. Additionally, the abundance of biosurfactant-producing and hydrocarbon-degrading bacteria increased significantly. This research and the application of the <i>P. aeruginosa</i> and <i>B. subtilis</i> co-culture system provide novel insights and strategies for MEOR.https://www.mdpi.com/2076-2607/12/11/2343co-culturebiosurfactant productionmicrobial enhanced oil recoverycooperation mechanismresidual oil mobilizationfield trial |
| spellingShingle | Dingyu Kang Hai Lin Qiang Li Nan Su Changkun Cheng Yijing Luo Zhongzhi Zhang Zhiyong Zhang Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> Microorganisms co-culture biosurfactant production microbial enhanced oil recovery cooperation mechanism residual oil mobilization field trial |
| title | Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> |
| title_full | Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> |
| title_fullStr | Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> |
| title_full_unstemmed | Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> |
| title_short | Enhanced Oil Recovery in a Co-Culture System of <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> |
| title_sort | enhanced oil recovery in a co culture system of i pseudomonas aeruginosa i and i bacillus subtilis i |
| topic | co-culture biosurfactant production microbial enhanced oil recovery cooperation mechanism residual oil mobilization field trial |
| url | https://www.mdpi.com/2076-2607/12/11/2343 |
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