Structural basis of RNA polymerase complexes in African swine fever virus
Abstract African swine fever virus is highly contagious and causes a fatal infectious disease in pigs, resulting in a significant global impact on pork supply. The African swine fever virus RNA polymerase serves as a crucial multifunctional protein complex responsible for genome transcription and re...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55683-z |
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| author | Guoliang Zhu Fei Xi Wuxia Zeng Yifei Zhao Weijun Cao Chen Liu Fan Yang Yi Ru Shuqi Xiao Shilei Zhang Huanan Liu Hong Tian Fayu Yang Biao Lu Shukai Sun Haiyang Song Bozhang Sun Xiaoyi Zhao Lijie Tang Kangli Li Jijun He Jianhong Guo Yun Zhu Zixiang Zhu Fei Sun Haixue Zheng |
| author_facet | Guoliang Zhu Fei Xi Wuxia Zeng Yifei Zhao Weijun Cao Chen Liu Fan Yang Yi Ru Shuqi Xiao Shilei Zhang Huanan Liu Hong Tian Fayu Yang Biao Lu Shukai Sun Haiyang Song Bozhang Sun Xiaoyi Zhao Lijie Tang Kangli Li Jijun He Jianhong Guo Yun Zhu Zixiang Zhu Fei Sun Haixue Zheng |
| author_sort | Guoliang Zhu |
| collection | DOAJ |
| description | Abstract African swine fever virus is highly contagious and causes a fatal infectious disease in pigs, resulting in a significant global impact on pork supply. The African swine fever virus RNA polymerase serves as a crucial multifunctional protein complex responsible for genome transcription and regulation. Therefore, it is essential to investigate its structural and functional characteristics for the prevention and control of African swine fever. Here, we determine the structures of endogenous African swine fever virus RNA polymerase in both nucleic acid-free and elongation states. The African swine fever virus RNA polymerase shares similarities with the core of typical RNA polymerases, but possesses a distinct subunit M1249L. Notably, the dynamic binding mode of M1249L with RNA polymerase, along with the C-terminal tail insertion of M1249L in the active center of DNA-RNA scaffold binding, suggests the potential of M1249L to regulate RNA polymerase activity within cells. These results are important for understanding the transcription cycle of the African swine fever virus and for developing antiviral strategies. |
| format | Article |
| id | doaj-art-dccef9e551c7499d8bc5934daf4a0ce7 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-dccef9e551c7499d8bc5934daf4a0ce72025-01-12T12:30:48ZengNature PortfolioNature Communications2041-17232025-01-0116111310.1038/s41467-024-55683-zStructural basis of RNA polymerase complexes in African swine fever virusGuoliang Zhu0Fei Xi1Wuxia Zeng2Yifei Zhao3Weijun Cao4Chen Liu5Fan Yang6Yi Ru7Shuqi Xiao8Shilei Zhang9Huanan Liu10Hong Tian11Fayu Yang12Biao Lu13Shukai Sun14Haiyang Song15Bozhang Sun16Xiaoyi Zhao17Lijie Tang18Kangli Li19Jijun He20Jianhong Guo21Yun Zhu22Zixiang Zhu23Fei Sun24Haixue Zheng25State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural UniversityState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesAbstract African swine fever virus is highly contagious and causes a fatal infectious disease in pigs, resulting in a significant global impact on pork supply. The African swine fever virus RNA polymerase serves as a crucial multifunctional protein complex responsible for genome transcription and regulation. Therefore, it is essential to investigate its structural and functional characteristics for the prevention and control of African swine fever. Here, we determine the structures of endogenous African swine fever virus RNA polymerase in both nucleic acid-free and elongation states. The African swine fever virus RNA polymerase shares similarities with the core of typical RNA polymerases, but possesses a distinct subunit M1249L. Notably, the dynamic binding mode of M1249L with RNA polymerase, along with the C-terminal tail insertion of M1249L in the active center of DNA-RNA scaffold binding, suggests the potential of M1249L to regulate RNA polymerase activity within cells. These results are important for understanding the transcription cycle of the African swine fever virus and for developing antiviral strategies.https://doi.org/10.1038/s41467-024-55683-z |
| spellingShingle | Guoliang Zhu Fei Xi Wuxia Zeng Yifei Zhao Weijun Cao Chen Liu Fan Yang Yi Ru Shuqi Xiao Shilei Zhang Huanan Liu Hong Tian Fayu Yang Biao Lu Shukai Sun Haiyang Song Bozhang Sun Xiaoyi Zhao Lijie Tang Kangli Li Jijun He Jianhong Guo Yun Zhu Zixiang Zhu Fei Sun Haixue Zheng Structural basis of RNA polymerase complexes in African swine fever virus Nature Communications |
| title | Structural basis of RNA polymerase complexes in African swine fever virus |
| title_full | Structural basis of RNA polymerase complexes in African swine fever virus |
| title_fullStr | Structural basis of RNA polymerase complexes in African swine fever virus |
| title_full_unstemmed | Structural basis of RNA polymerase complexes in African swine fever virus |
| title_short | Structural basis of RNA polymerase complexes in African swine fever virus |
| title_sort | structural basis of rna polymerase complexes in african swine fever virus |
| url | https://doi.org/10.1038/s41467-024-55683-z |
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