Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice

Optoacoustic (OA) tomography is a powerful noninvasive preclinical imaging tool enabling high resolution whole-body visualization of biodistribution and dynamics of molecular agents. The technique yet lacks endogenous soft-tissue contrast, which often hampers anatomical navigation. Herein, we devise...

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Main Authors: Sandeep Kumar Kalva, Ali Özbek, Michael Reiss, Xosé Luís Deán-Ben, Daniel Razansky
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Photoacoustics
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213597924000764
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author Sandeep Kumar Kalva
Ali Özbek
Michael Reiss
Xosé Luís Deán-Ben
Daniel Razansky
author_facet Sandeep Kumar Kalva
Ali Özbek
Michael Reiss
Xosé Luís Deán-Ben
Daniel Razansky
author_sort Sandeep Kumar Kalva
collection DOAJ
description Optoacoustic (OA) tomography is a powerful noninvasive preclinical imaging tool enabling high resolution whole-body visualization of biodistribution and dynamics of molecular agents. The technique yet lacks endogenous soft-tissue contrast, which often hampers anatomical navigation. Herein, we devise spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography for concurrent OA and pulse-echo ultrasound (US) imaging of whole mice. To this end, a spherical array transducer featuring a central curvilinear segment is employed. Full rotation of the array renders transverse US and OA views, while additional translation facilitates volumetric whole-body imaging with high spatial resolution down to 150 µm and 110 µm in the OA and US modes, respectively. OA imaging revealed blood-filled, vascular organs like heart, liver, spleen, kidneys, and surrounding vasculature, whilst complementary details of bones, lungs, and skin boundaries were provided by the US. The dual-modal capability of SVOPUS for label-free imaging of tissue morphology and function is poised to facilitate pharmacokinetic studies, disease monitoring, and image-guided therapies.
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institution Kabale University
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publishDate 2024-12-01
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series Photoacoustics
spelling doaj-art-dca6518c504b44b09ca19fd5eb7311d62024-11-20T05:06:56ZengElsevierPhotoacoustics2213-59792024-12-0140100659Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of miceSandeep Kumar Kalva0Ali Özbek1Michael Reiss2Xosé Luís Deán-Ben3Daniel Razansky4Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, Switzerland; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, IndiaInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, SwitzerlandInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, SwitzerlandInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, SwitzerlandInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, Switzerland; Correspondence to: Institute of Biomedical Engineering, University of Zurich and ETH Zurich, HIT E42.1, Wolfgang-Pauli-Strasse 27, Zurich CH-8093, Switzerland.Optoacoustic (OA) tomography is a powerful noninvasive preclinical imaging tool enabling high resolution whole-body visualization of biodistribution and dynamics of molecular agents. The technique yet lacks endogenous soft-tissue contrast, which often hampers anatomical navigation. Herein, we devise spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography for concurrent OA and pulse-echo ultrasound (US) imaging of whole mice. To this end, a spherical array transducer featuring a central curvilinear segment is employed. Full rotation of the array renders transverse US and OA views, while additional translation facilitates volumetric whole-body imaging with high spatial resolution down to 150 µm and 110 µm in the OA and US modes, respectively. OA imaging revealed blood-filled, vascular organs like heart, liver, spleen, kidneys, and surrounding vasculature, whilst complementary details of bones, lungs, and skin boundaries were provided by the US. The dual-modal capability of SVOPUS for label-free imaging of tissue morphology and function is poised to facilitate pharmacokinetic studies, disease monitoring, and image-guided therapies.http://www.sciencedirect.com/science/article/pii/S2213597924000764Optoacoustic imagingPhotoacousticsSmall animal imagingUltrasonographyUltrasonic transducers
spellingShingle Sandeep Kumar Kalva
Ali Özbek
Michael Reiss
Xosé Luís Deán-Ben
Daniel Razansky
Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice
Photoacoustics
Optoacoustic imaging
Photoacoustics
Small animal imaging
Ultrasonography
Ultrasonic transducers
title Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice
title_full Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice
title_fullStr Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice
title_full_unstemmed Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice
title_short Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice
title_sort spiral volumetric optoacoustic and ultrasound svopus tomography of mice
topic Optoacoustic imaging
Photoacoustics
Small animal imaging
Ultrasonography
Ultrasonic transducers
url http://www.sciencedirect.com/science/article/pii/S2213597924000764
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