Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis
Background: The differential diagnosis of tuberculosis (TB) and lung cancer (LC) is often challenging due to similar clinicopathological presentations when bacterial shedding is negative, which can lead to delays in treatment. In this study, we tested the potential of plasma-circulating microRNAs (m...
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2024-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/14/23/2684 |
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| author | Yeldar Ashirbekov Nazgul Khamitova Kantemir Satken Arman Abaildayev Ilya Pinskiy Askar Yeleussizov Laura Yegenova Anargul Kairanbayeva Danara Kadirshe Gulzhakhan Utegenova Nurlan Jainakbayev Kamalidin Sharipov |
| author_facet | Yeldar Ashirbekov Nazgul Khamitova Kantemir Satken Arman Abaildayev Ilya Pinskiy Askar Yeleussizov Laura Yegenova Anargul Kairanbayeva Danara Kadirshe Gulzhakhan Utegenova Nurlan Jainakbayev Kamalidin Sharipov |
| author_sort | Yeldar Ashirbekov |
| collection | DOAJ |
| description | Background: The differential diagnosis of tuberculosis (TB) and lung cancer (LC) is often challenging due to similar clinicopathological presentations when bacterial shedding is negative, which can lead to delays in treatment. In this study, we tested the potential of plasma-circulating microRNAs (miRNAs) for the early and differential diagnosis of TB and LC. Methods: We conducted a two-phase study: profiling 188 miRNAs in pooled plasma samples and validating 14 selected miRNAs in individual plasma samples from 68 LC patients, 38 pulmonary TB patients, and 41 healthy controls. Results: Twelve miRNAs were significantly elevated in LC patients compared to controls and TB patients, while two miRNAs were significantly elevated in TB patients compared to controls. ROC analysis demonstrated that miR-130b-3p, miR-1-3p, miR-423-5p, and miR-200a-3p had good discriminatory ability to distinguish LC patients (including those with stage I tumours) from healthy individuals and miR-130b-3p, miR-423-5p, miR-15b-5p, and miR-18b-5p effectively distinguished LC patients (including those with stage I tumours) from TB patients. Additionally, miR-18b-5p showed good discriminatory ability between SCLC and NSCLC patients. Conclusions: Circulating miRNAs hold strong potential for the early detection of LC and for distinguishing LC from TB. |
| format | Article |
| id | doaj-art-dc8180e0aa7a46bcae2268ed1c679e70 |
| institution | Kabale University |
| issn | 2075-4418 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj-art-dc8180e0aa7a46bcae2268ed1c679e702024-12-13T16:24:39ZengMDPI AGDiagnostics2075-44182024-11-011423268410.3390/diagnostics14232684Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from TuberculosisYeldar Ashirbekov0Nazgul Khamitova1Kantemir Satken2Arman Abaildayev3Ilya Pinskiy4Askar Yeleussizov5Laura Yegenova6Anargul Kairanbayeva7Danara Kadirshe8Gulzhakhan Utegenova9Nurlan Jainakbayev10Kamalidin Sharipov11Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, KazakhstanAitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, KazakhstanAitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, KazakhstanAitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, KazakhstanAl-Farabi Kazakh National University, Almaty 050040, KazakhstanKazakh Institute of Oncology and Radiology, Almaty 050012, KazakhstanNational Scientific Center of Phthisiopulmonology, Almaty 050010, KazakhstanNational Scientific Center of Phthisiopulmonology, Almaty 050010, KazakhstanAitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, KazakhstanAitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, KazakhstanKazakh-Russian Medical University, Almaty 050004, KazakhstanAitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, KazakhstanBackground: The differential diagnosis of tuberculosis (TB) and lung cancer (LC) is often challenging due to similar clinicopathological presentations when bacterial shedding is negative, which can lead to delays in treatment. In this study, we tested the potential of plasma-circulating microRNAs (miRNAs) for the early and differential diagnosis of TB and LC. Methods: We conducted a two-phase study: profiling 188 miRNAs in pooled plasma samples and validating 14 selected miRNAs in individual plasma samples from 68 LC patients, 38 pulmonary TB patients, and 41 healthy controls. Results: Twelve miRNAs were significantly elevated in LC patients compared to controls and TB patients, while two miRNAs were significantly elevated in TB patients compared to controls. ROC analysis demonstrated that miR-130b-3p, miR-1-3p, miR-423-5p, and miR-200a-3p had good discriminatory ability to distinguish LC patients (including those with stage I tumours) from healthy individuals and miR-130b-3p, miR-423-5p, miR-15b-5p, and miR-18b-5p effectively distinguished LC patients (including those with stage I tumours) from TB patients. Additionally, miR-18b-5p showed good discriminatory ability between SCLC and NSCLC patients. Conclusions: Circulating miRNAs hold strong potential for the early detection of LC and for distinguishing LC from TB.https://www.mdpi.com/2075-4418/14/23/2684lung cancertuberculosisdifferential diagnosismicroRNA |
| spellingShingle | Yeldar Ashirbekov Nazgul Khamitova Kantemir Satken Arman Abaildayev Ilya Pinskiy Askar Yeleussizov Laura Yegenova Anargul Kairanbayeva Danara Kadirshe Gulzhakhan Utegenova Nurlan Jainakbayev Kamalidin Sharipov Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis Diagnostics lung cancer tuberculosis differential diagnosis microRNA |
| title | Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis |
| title_full | Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis |
| title_fullStr | Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis |
| title_full_unstemmed | Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis |
| title_short | Circulating MicroRNAs as Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis |
| title_sort | circulating micrornas as biomarkers for the early diagnosis of lung cancer and its differentiation from tuberculosis |
| topic | lung cancer tuberculosis differential diagnosis microRNA |
| url | https://www.mdpi.com/2075-4418/14/23/2684 |
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