Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates
A study on the phytochemistry and antibacterial effects of petroleum ether, chloroform and methanol extract of flower of Azadirachta indica on five clinical isolates viz: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae was carried out usi...
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Umaru Musa Yar'adua University, Katsina, Nigeria
2016-12-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/339 |
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| author | Abdullahi, A. Nafiu, S.A Ishaq, S.A. Alkali, Z. D Lambu, Z. N. |
| author_facet | Abdullahi, A. Nafiu, S.A Ishaq, S.A. Alkali, Z. D Lambu, Z. N. |
| author_sort | Abdullahi, A. |
| collection | DOAJ |
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A study on the phytochemistry and antibacterial effects of petroleum ether, chloroform and methanol extract of flower of Azadirachta indica on five clinical isolates viz: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae was carried out using deep well agar diffusion and broth dilution methods. The results obtained showed that methanol flower extract possessed the highest antibacterial activity against P. miravilis with inhibition zone of 23mm at the concentration of 4000μg/ml. The minimum inhibitory concentration (MIC) of the petroleum ether flower extract was 62.5μg/ml for all the test organisms while P. miravilis, S. aureus and K. pneumoniae had MIC value 62.5μg/ml with the exception of E. coli and P. aeruginosa.Methanol flower extract recorded 62.5μg/ml against P. miravilis, S. aureus. While E. coli, P. aeruginosa and K. pneumoniae had no MIC value across all the concentrations. the minimum bactericidal concentration (MBC) for the petroleum ether extracts was 125μg/ml, for chloroform extracts was 250μg/ml and for methanol extracts was 62.5μg/ml. Phytochemical analysis of the flower extracts showed the presence of reducing sugars, alkaloids, tannins, flavonoids, resins, and saponins, in all the extracts. Petroleum ether flower extract (PFE) showed antibacterial activities against all the test organisms while Methanol flower extract (MFE) had antibacterial activities against only two test organisms.
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| format | Article |
| id | doaj-art-dc54ab52d8b84680a0f0ca41b6288ccb |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-dc54ab52d8b84680a0f0ca41b6288ccb2024-11-28T11:10:09ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222016-12-011110.47430/ujmr.1611.009339Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical IsolatesAbdullahi, A.0Nafiu, S.A1Ishaq, S.A.2Alkali, Z. D3Lambu, Z. N.4Department of Science Laboratory Technology, School of Technology, Kano state Polytechnic.Department of Science Laboratory Technology, School of Technology, Kano state Polytechnic.Department of Biology, School of Sciences, FCE (T) Bichi, Kano State Department of Science Laboratory Technology, School of Technology, Kano state Polytechnic.Department of Microbiology, Kano State University of Science and Technology Wudil, A study on the phytochemistry and antibacterial effects of petroleum ether, chloroform and methanol extract of flower of Azadirachta indica on five clinical isolates viz: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae was carried out using deep well agar diffusion and broth dilution methods. The results obtained showed that methanol flower extract possessed the highest antibacterial activity against P. miravilis with inhibition zone of 23mm at the concentration of 4000μg/ml. The minimum inhibitory concentration (MIC) of the petroleum ether flower extract was 62.5μg/ml for all the test organisms while P. miravilis, S. aureus and K. pneumoniae had MIC value 62.5μg/ml with the exception of E. coli and P. aeruginosa.Methanol flower extract recorded 62.5μg/ml against P. miravilis, S. aureus. While E. coli, P. aeruginosa and K. pneumoniae had no MIC value across all the concentrations. the minimum bactericidal concentration (MBC) for the petroleum ether extracts was 125μg/ml, for chloroform extracts was 250μg/ml and for methanol extracts was 62.5μg/ml. Phytochemical analysis of the flower extracts showed the presence of reducing sugars, alkaloids, tannins, flavonoids, resins, and saponins, in all the extracts. Petroleum ether flower extract (PFE) showed antibacterial activities against all the test organisms while Methanol flower extract (MFE) had antibacterial activities against only two test organisms. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/339PhytochemistryAzadirachta indicaextractsClinical isolatesMICMBC |
| spellingShingle | Abdullahi, A. Nafiu, S.A Ishaq, S.A. Alkali, Z. D Lambu, Z. N. Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates UMYU Journal of Microbiology Research Phytochemistry Azadirachta indica extracts Clinical isolates MIC MBC |
| title | Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates |
| title_full | Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates |
| title_fullStr | Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates |
| title_full_unstemmed | Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates |
| title_short | Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates |
| title_sort | antibacterial activities and phytochemical screening of extracts of flower of azadiracthta indica neem against some selected clinical isolates |
| topic | Phytochemistry Azadirachta indica extracts Clinical isolates MIC MBC |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/339 |
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