COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO

COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO

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<p><strong>Background:</strong> Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment.</p><p>In...

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Main Authors:	Fathiah Zakham, Oufae Lahlou, Mohammed Akrim, Nada Bouklata, Sanae Jaouhari, Khalid Sadki, Fouad Seghrouchni, Mohammed Elmzibri, Abdelaziz Benjouad, Moulay Mustapha Ennaji, Rajae Elaouad
Format:	Article
Language:	English
Published:	PAGEPress Publications 2012-01-01
Series:	Mediterranean Journal of Hematology and Infectious Diseases
Subjects:	Tuberculosis Mycobacterium tuberculosis PCR, IS6110
Online Access:	http://www.mjhid.org/article/view/10420
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Summary:	<p><strong>Background:</strong> Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment.</p><p>In the objective of early TB diagnosis and rapid detection of <em>Mycobacterium tuberculosis </em>(MTB) in the clinical specimens, the utility of the Polymerase Chain Reaction (PCR) using the Insertion Sequence 6110 (IS6110) as target was compared to conventional methods.</p><p><strong>Methods:</strong> Out of 305 patients with different clinical manifestations: suspected, new, drug relapse, drug failure and chronic cases were enrolled in this study and tested by mycobacteriological and PCR techniques for the investigation about the tubercle bacilli.</p><p><strong>Results:</strong> The results of the in house IS6110 PCR showed a good sensitivity (92, 42%) and high specificity (98%), the positive and negative predictive values were 96.4 % and 95.3 % respectively.</p><p><strong>Conclusion:</strong> This study showed clearly that the PCR testing using the IS6110 in the routine analysis is a potential tool for the rapid TB diagnosis, especially for critical cases and would be of great interest to help the clinician in the misdiagnosed critical cases by the traditional radiology.</p>
ISSN:	2035-3006