A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for Moxifloxacin

In the present study, a partial least square regression (PLSR) was applied to Fourier-transform infrared (FTIR) data for rapid and direct quantification of a quinolone family antibiotic moxifloxacin and the proposed method was counter validated by reverse-phase high-performance liquid chromatography...

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Main Authors: Muhammad Umair Kamal, Sumia Akram, Muhammad Ahmad Khan, Atique Ur Rehman, Muhammad Ehsan Ameer, Raja Adil Sarfraz, Asif Iqbal, Rizwan Ashraf, Muhammad Mushtaq
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Journal of Spectroscopy
Online Access:http://dx.doi.org/10.1155/jspe/6448992
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author Muhammad Umair Kamal
Sumia Akram
Muhammad Ahmad Khan
Atique Ur Rehman
Muhammad Ehsan Ameer
Raja Adil Sarfraz
Asif Iqbal
Rizwan Ashraf
Muhammad Mushtaq
author_facet Muhammad Umair Kamal
Sumia Akram
Muhammad Ahmad Khan
Atique Ur Rehman
Muhammad Ehsan Ameer
Raja Adil Sarfraz
Asif Iqbal
Rizwan Ashraf
Muhammad Mushtaq
author_sort Muhammad Umair Kamal
collection DOAJ
description In the present study, a partial least square regression (PLSR) was applied to Fourier-transform infrared (FTIR) data for rapid and direct quantification of a quinolone family antibiotic moxifloxacin and the proposed method was counter validated by reverse-phase high-performance liquid chromatography under diode array detection (RP-HPLC-DAD). The PLSR-based processing of FTIR spectra of moxifloxacin standards and formulations disclosed a concentration-dependent mid-IR region at 1456 cm−1. The FTIR-based assay method was validated in accordance with the International Conference on Harmonization (ICH) guidelines and was found to be linear over the concentration range of 0.1 mg/mg–1.0 mg/mg (w/w) of solid mixtures with a coefficient of determination (R2) of 0.9951. Moreover, the results when counter validated by RP-HPLC-DAD were found to be concordant (RSD ≤ 2%). The analysis of moxifloxacin quality control samples through the developed FTIR assay offered overall recovery rates equals to 97.61%–103.74%. The real-time analysis of moxifloxacin formulations marketed in Pakistan authenticated the presence of active pharmaceutical ingredients (APIs) within the claimed ranges. We can eventually conclude that FTIR spectroscopy if coupled with a suitable data-processing scheme can provide more expedient, rapid, and economical choice for the direct quantification of moxifloxacin API and its formulations.
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spelling doaj-art-dbfd4001afc24085ad3bc821fda5b8a82025-08-20T03:46:53ZengWileyJournal of Spectroscopy2314-49392025-01-01202510.1155/jspe/6448992A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for MoxifloxacinMuhammad Umair Kamal0Sumia Akram1Muhammad Ahmad Khan2Atique Ur Rehman3Muhammad Ehsan Ameer4Raja Adil Sarfraz5Asif Iqbal6Rizwan Ashraf7Muhammad Mushtaq8Department of ChemistryDivision of Science and TechnologyDepartment of ChemistryUniversity of Child Heath Science LahoreDepartment of ChemistryDepartment of ChemistryDepartment of ChemistryDepartment of ChemistryDepartment of ChemistryIn the present study, a partial least square regression (PLSR) was applied to Fourier-transform infrared (FTIR) data for rapid and direct quantification of a quinolone family antibiotic moxifloxacin and the proposed method was counter validated by reverse-phase high-performance liquid chromatography under diode array detection (RP-HPLC-DAD). The PLSR-based processing of FTIR spectra of moxifloxacin standards and formulations disclosed a concentration-dependent mid-IR region at 1456 cm−1. The FTIR-based assay method was validated in accordance with the International Conference on Harmonization (ICH) guidelines and was found to be linear over the concentration range of 0.1 mg/mg–1.0 mg/mg (w/w) of solid mixtures with a coefficient of determination (R2) of 0.9951. Moreover, the results when counter validated by RP-HPLC-DAD were found to be concordant (RSD ≤ 2%). The analysis of moxifloxacin quality control samples through the developed FTIR assay offered overall recovery rates equals to 97.61%–103.74%. The real-time analysis of moxifloxacin formulations marketed in Pakistan authenticated the presence of active pharmaceutical ingredients (APIs) within the claimed ranges. We can eventually conclude that FTIR spectroscopy if coupled with a suitable data-processing scheme can provide more expedient, rapid, and economical choice for the direct quantification of moxifloxacin API and its formulations.http://dx.doi.org/10.1155/jspe/6448992
spellingShingle Muhammad Umair Kamal
Sumia Akram
Muhammad Ahmad Khan
Atique Ur Rehman
Muhammad Ehsan Ameer
Raja Adil Sarfraz
Asif Iqbal
Rizwan Ashraf
Muhammad Mushtaq
A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for Moxifloxacin
Journal of Spectroscopy
title A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for Moxifloxacin
title_full A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for Moxifloxacin
title_fullStr A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for Moxifloxacin
title_full_unstemmed A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for Moxifloxacin
title_short A Fourier-Transform Infrared Spectroscopy-Based Simple, Rapid, and Direct Quantification Assay for Moxifloxacin
title_sort fourier transform infrared spectroscopy based simple rapid and direct quantification assay for moxifloxacin
url http://dx.doi.org/10.1155/jspe/6448992
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