Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2)
Sensitization to cross-reactive allergens complicates identifying the culprit insect in Hymenoptera venom allergy via diagnostic tests. This study evaluates sensitization to hyaluronidases (Api m 2 from honey bee (<i>Apis mellifera</i>) venom, HBV; Pol d 2 from European paper wasp (<i...
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2024-11-01
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| author | Johannes Grosch Bernadette Eberlein Sebastian Waldherr Mariona Pascal Britta Dorn Clara San Bartolomé Federico De La Roca Pinzón Maximilian Schiener Ulf Darsow Tilo Biedermann Jonas Lidholm Maria Beatrice Bilò Thilo Jakob Carsten B. Schmidt-Weber Simon Blank |
| author_facet | Johannes Grosch Bernadette Eberlein Sebastian Waldherr Mariona Pascal Britta Dorn Clara San Bartolomé Federico De La Roca Pinzón Maximilian Schiener Ulf Darsow Tilo Biedermann Jonas Lidholm Maria Beatrice Bilò Thilo Jakob Carsten B. Schmidt-Weber Simon Blank |
| author_sort | Johannes Grosch |
| collection | DOAJ |
| description | Sensitization to cross-reactive allergens complicates identifying the culprit insect in Hymenoptera venom allergy via diagnostic tests. This study evaluates sensitization to hyaluronidases (Api m 2 from honey bee (<i>Apis mellifera</i>) venom, HBV; Pol d 2 from European paper wasp (<i>Polistes dominula</i>) venom, PDV; and Ves v 2.0101 and Ves v 2.0201 from yellow jacket (<i>Vespula vulgaris</i>) venom, YJV) and their cross-reactivity in allergic patients from Italy, Spain, and Germany using ImmunoCAPs, ELISA, and basophil activation tests. Sensitization rates were 45% for Api m 2 in HBV-allergic subjects, 25% for Pol d 2 in PDV-allergic individuals, and 20% and 10% for Ves v 2.0201 and Ves v 2.0101 in YJV-allergic patients, respectively. Patients primarily sensitized to Api m 2 showed minimal cross-reactivity to vespid hyaluronidases, whereas those primarily sensitized to Pol d 2 or Ves v 2.0201 exhibited IgE reactivity to Api m 2. Neither Pol d 2 nor Ves v 2.0201 triggered basophil activation. Cross-reactivity of Api m 2, Pol d 2, and Ves v 2.0201 depends on the primary sensitizing venom. Sensitization to Pol d 2 and Ves v 2.0201 remains below 25%, yet these patients may exhibit cross-reactivity to Api m 2. Conversely, HBV-allergic patients sensitized to Api m 2 show minimal reactivity to Pol d 2 or Ves v 2.0201. |
| format | Article |
| id | doaj-art-dbab6879640c47e9aaf3b272aae159c8 |
| institution | Kabale University |
| issn | 2072-6651 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj-art-dbab6879640c47e9aaf3b272aae159c82024-11-26T18:24:10ZengMDPI AGToxins2072-66512024-11-01161149810.3390/toxins16110498Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2)Johannes Grosch0Bernadette Eberlein1Sebastian Waldherr2Mariona Pascal3Britta Dorn4Clara San Bartolomé5Federico De La Roca Pinzón6Maximilian Schiener7Ulf Darsow8Tilo Biedermann9Jonas Lidholm10Maria Beatrice Bilò11Thilo Jakob12Carsten B. Schmidt-Weber13Simon Blank14Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, GermanyDepartment of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, GermanyDepartment of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, GermanyImmunology Department, CDB Hospital Clinic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, 08007 Barcelona, SpainExperimental Dermatology and Allergy Research Group, Department of Dermatology and Allergology, Justus-Liebig-University Gießen, 35392 Gießen, GermanyImmunology Department, CDB Hospital Clinic de Barcelona, Universitat de Barcelona, 08007 Barcelona, SpainAllergy Section, Hospital Clinic de Barcelona, Clinical Medical Group, 08036 Barcelona, SpainCenter of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, GermanyDepartment of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, GermanyDepartment of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, GermanyThermo Fisher Scientific, 75450 Uppsala, SwedenDepartment of Clinical and Molecular Sciences, Polytechnic University of Marche, 60121 Ancona, ItalyExperimental Dermatology and Allergy Research Group, Department of Dermatology and Allergology, Justus-Liebig-University Gießen, 35392 Gießen, GermanyCenter of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, GermanyCenter of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, GermanySensitization to cross-reactive allergens complicates identifying the culprit insect in Hymenoptera venom allergy via diagnostic tests. This study evaluates sensitization to hyaluronidases (Api m 2 from honey bee (<i>Apis mellifera</i>) venom, HBV; Pol d 2 from European paper wasp (<i>Polistes dominula</i>) venom, PDV; and Ves v 2.0101 and Ves v 2.0201 from yellow jacket (<i>Vespula vulgaris</i>) venom, YJV) and their cross-reactivity in allergic patients from Italy, Spain, and Germany using ImmunoCAPs, ELISA, and basophil activation tests. Sensitization rates were 45% for Api m 2 in HBV-allergic subjects, 25% for Pol d 2 in PDV-allergic individuals, and 20% and 10% for Ves v 2.0201 and Ves v 2.0101 in YJV-allergic patients, respectively. Patients primarily sensitized to Api m 2 showed minimal cross-reactivity to vespid hyaluronidases, whereas those primarily sensitized to Pol d 2 or Ves v 2.0201 exhibited IgE reactivity to Api m 2. Neither Pol d 2 nor Ves v 2.0201 triggered basophil activation. Cross-reactivity of Api m 2, Pol d 2, and Ves v 2.0201 depends on the primary sensitizing venom. Sensitization to Pol d 2 and Ves v 2.0201 remains below 25%, yet these patients may exhibit cross-reactivity to Api m 2. Conversely, HBV-allergic patients sensitized to Api m 2 show minimal reactivity to Pol d 2 or Ves v 2.0201.https://www.mdpi.com/2072-6651/16/11/498allergen cross-reactivityApi m 2<i>Apis mellifera</i>hyaluronidasesHymenoptera venom allergyPol d 2 |
| spellingShingle | Johannes Grosch Bernadette Eberlein Sebastian Waldherr Mariona Pascal Britta Dorn Clara San Bartolomé Federico De La Roca Pinzón Maximilian Schiener Ulf Darsow Tilo Biedermann Jonas Lidholm Maria Beatrice Bilò Thilo Jakob Carsten B. Schmidt-Weber Simon Blank Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2) Toxins allergen cross-reactivity Api m 2 <i>Apis mellifera</i> hyaluronidases Hymenoptera venom allergy Pol d 2 |
| title | Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2) |
| title_full | Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2) |
| title_fullStr | Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2) |
| title_full_unstemmed | Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2) |
| title_short | Comparative Assessment of the Allergenicity of Hyaluronidases from <i>Polistes dominula</i> (Pol d 2), <i>Vespula vulgaris</i> (Ves v 2), and <i>Apis mellifera</i> Venom (Api m 2) |
| title_sort | comparative assessment of the allergenicity of hyaluronidases from i polistes dominula i pol d 2 i vespula vulgaris i ves v 2 and i apis mellifera i venom api m 2 |
| topic | allergen cross-reactivity Api m 2 <i>Apis mellifera</i> hyaluronidases Hymenoptera venom allergy Pol d 2 |
| url | https://www.mdpi.com/2072-6651/16/11/498 |
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