WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B Stress
The depletion of the ozone layer has resulted in elevated ultraviolet-B (UV-B) radiation levels, posing a significant risk to terrestrial plant growth. <i>Rhododendron chrysanthum</i> Pall. (<i>R. chrysanthum</i>), adapted to high-altitude and high-irradiation environments, h...
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2025-01-01
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| author | Wang Yu Xiangru Zhou Jinhao Meng Hongwei Xu Xiaofu Zhou |
| author_facet | Wang Yu Xiangru Zhou Jinhao Meng Hongwei Xu Xiaofu Zhou |
| author_sort | Wang Yu |
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| description | The depletion of the ozone layer has resulted in elevated ultraviolet-B (UV-B) radiation levels, posing a significant risk to terrestrial plant growth. <i>Rhododendron chrysanthum</i> Pall. (<i>R. chrysanthum</i>), adapted to high-altitude and high-irradiation environments, has developed unique adaptive mechanisms. This study exposed <i>R. chrysanthum</i> to UV-B radiation for two days, with an 8 h daily treatment, utilizing metabolomic and transcriptomic analyses to explore the role of WRKY transcription factors in the plant’s UV-B stress response and their regulation of flavonoid synthesis. UV-B stress resulted in a significant decrease in rETR and Ik and a significant increase in 1-qP. These chlorophyll fluorescence parameters indicate that UV-B stress impaired photosynthesis in <i>R. chrysanthum</i>. Faced with the detrimental impact of UV-B radiation, <i>R. chrysanthum</i> is capable of mitigating its effects by modulating its flavonoid biosynthetic pathways to adapt positively to the stress. This study revealed changes in the expression of 113 flavonoid-related metabolites and 42 associated genes, with WRKY transcription factors showing significant correlation with these alterations. WRKY transcription factors can influence the expression of key enzyme genes in the flavonoid metabolic pathway, thereby affecting metabolite production. A theoretical reference for investigating plant stress physiology is provided in this work, which also offers insights into the stress responses of alpine plants under adverse conditions. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-db830ec79fc849009d3d1a02588ad39c2025-01-10T13:19:51ZengMDPI AGPlants2223-77472025-01-0114113310.3390/plants14010133WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B StressWang Yu0Xiangru Zhou1Jinhao Meng2Hongwei Xu3Xiaofu Zhou4Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaJilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaJilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaJilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaJilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, ChinaThe depletion of the ozone layer has resulted in elevated ultraviolet-B (UV-B) radiation levels, posing a significant risk to terrestrial plant growth. <i>Rhododendron chrysanthum</i> Pall. (<i>R. chrysanthum</i>), adapted to high-altitude and high-irradiation environments, has developed unique adaptive mechanisms. This study exposed <i>R. chrysanthum</i> to UV-B radiation for two days, with an 8 h daily treatment, utilizing metabolomic and transcriptomic analyses to explore the role of WRKY transcription factors in the plant’s UV-B stress response and their regulation of flavonoid synthesis. UV-B stress resulted in a significant decrease in rETR and Ik and a significant increase in 1-qP. These chlorophyll fluorescence parameters indicate that UV-B stress impaired photosynthesis in <i>R. chrysanthum</i>. Faced with the detrimental impact of UV-B radiation, <i>R. chrysanthum</i> is capable of mitigating its effects by modulating its flavonoid biosynthetic pathways to adapt positively to the stress. This study revealed changes in the expression of 113 flavonoid-related metabolites and 42 associated genes, with WRKY transcription factors showing significant correlation with these alterations. WRKY transcription factors can influence the expression of key enzyme genes in the flavonoid metabolic pathway, thereby affecting metabolite production. A theoretical reference for investigating plant stress physiology is provided in this work, which also offers insights into the stress responses of alpine plants under adverse conditions.https://www.mdpi.com/2223-7747/14/1/133<i>Rhododendron chrysanthum</i> Pall.multi-omicsWRKY transcription factorUV-B stressmetabolomics |
| spellingShingle | Wang Yu Xiangru Zhou Jinhao Meng Hongwei Xu Xiaofu Zhou WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B Stress Plants <i>Rhododendron chrysanthum</i> Pall. multi-omics WRKY transcription factor UV-B stress metabolomics |
| title | WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B Stress |
| title_full | WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B Stress |
| title_fullStr | WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B Stress |
| title_full_unstemmed | WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B Stress |
| title_short | WRKY Transcription Factors Modulate the Flavonoid Pathway of <i>Rhododendron chrysanthum</i> Pall. Under UV-B Stress |
| title_sort | wrky transcription factors modulate the flavonoid pathway of i rhododendron chrysanthum i pall under uv b stress |
| topic | <i>Rhododendron chrysanthum</i> Pall. multi-omics WRKY transcription factor UV-B stress metabolomics |
| url | https://www.mdpi.com/2223-7747/14/1/133 |
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