Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway
Objective(s): This research examined the protective function of salidroside (SAL) against angiotensin II (Ang II)-infused myocardial fibrosis and its associated mechanism.Materials and Methods: The C57BL/6 male murine models (n=24) received either saline solution or Ang II (1500 ng/kg/day) subcutane...
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Mashhad University of Medical Sciences
2025-06-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_25729_ac5a2b9b6bc377109be13f1e22f4e0e3.pdf |
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| author | Xi Zhu Zhen Hai Zhongping Ning |
| author_facet | Xi Zhu Zhen Hai Zhongping Ning |
| author_sort | Xi Zhu |
| collection | DOAJ |
| description | Objective(s): This research examined the protective function of salidroside (SAL) against angiotensin II (Ang II)-infused myocardial fibrosis and its associated mechanism.Materials and Methods: The C57BL/6 male murine models (n=24) received either saline solution or Ang II (1500 ng/kg/day) subcutaneously and an oral dosage of SAL (50 mg/kg/day) once daily for 28 days. Newborn Sprague-Dawley (SD) rats were used to isolate atrial fibroblasts.Results: The fibrotic region was raised by Ang II infusion, while SAL treatment inhibited it. Collagen I and III expression was raised by Ang II induction, but SAL therapy reduced their expression. SAL therapy also decreased the expression of other fibroblast differentiation-related markers induced by Ang II infusion. It elevated SIRT1, Nrf2, and HO-1 levels in atrial fibroblasts. Additionally, SAL significantly inhibited atrial fibroblasts, whereas EX527, an inhibitor of SIRT1, noticeably increased the migration ability. Furthermore, SAL suppressed intracellular ROS production and oxidative stress in Ang II-infused atrial fibroblasts. Conclusion: SAL protects against myocardial fibrosis infused by Ang II by activating the SIRT1-Nrf2 pathway. |
| format | Article |
| id | doaj-art-db3b478dbc304d8381b9a934dfe73aaa |
| institution | Kabale University |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-db3b478dbc304d8381b9a934dfe73aaa2025-08-20T03:56:45ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-06-0128681582410.22038/ijbms.2025.83659.1810525729Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathwayXi Zhu0Zhen Hai1Zhongping Ning2Department of Cardiology, Shanghai Pudong New Area Zhoupu Hospital (Shanghai Health Medical College Affiliated Zhoupu Hospital) Shanghai 201318, ChinaDepartment of Cardiology, Shanghai Pudong New Area Zhoupu Hospital (Shanghai Health Medical College Affiliated Zhoupu Hospital) Shanghai 201318, ChinaDepartment of Cardiology, Shanghai Pudong New Area Zhoupu Hospital (Shanghai Health Medical College Affiliated Zhoupu Hospital) Shanghai 201318, ChinaObjective(s): This research examined the protective function of salidroside (SAL) against angiotensin II (Ang II)-infused myocardial fibrosis and its associated mechanism.Materials and Methods: The C57BL/6 male murine models (n=24) received either saline solution or Ang II (1500 ng/kg/day) subcutaneously and an oral dosage of SAL (50 mg/kg/day) once daily for 28 days. Newborn Sprague-Dawley (SD) rats were used to isolate atrial fibroblasts.Results: The fibrotic region was raised by Ang II infusion, while SAL treatment inhibited it. Collagen I and III expression was raised by Ang II induction, but SAL therapy reduced their expression. SAL therapy also decreased the expression of other fibroblast differentiation-related markers induced by Ang II infusion. It elevated SIRT1, Nrf2, and HO-1 levels in atrial fibroblasts. Additionally, SAL significantly inhibited atrial fibroblasts, whereas EX527, an inhibitor of SIRT1, noticeably increased the migration ability. Furthermore, SAL suppressed intracellular ROS production and oxidative stress in Ang II-infused atrial fibroblasts. Conclusion: SAL protects against myocardial fibrosis infused by Ang II by activating the SIRT1-Nrf2 pathway.https://ijbms.mums.ac.ir/article_25729_ac5a2b9b6bc377109be13f1e22f4e0e3.pdfangiotensin iioxidative stressreactive oxygen speciessalidrosidesirt1 |
| spellingShingle | Xi Zhu Zhen Hai Zhongping Ning Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway Iranian Journal of Basic Medical Sciences angiotensin ii oxidative stress reactive oxygen species salidroside sirt1 |
| title | Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway |
| title_full | Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway |
| title_fullStr | Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway |
| title_full_unstemmed | Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway |
| title_short | Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway |
| title_sort | salidroside impedes ang ii infused myocardial fibrosis by activating the sirt1 nrf2 pathway |
| topic | angiotensin ii oxidative stress reactive oxygen species salidroside sirt1 |
| url | https://ijbms.mums.ac.ir/article_25729_ac5a2b9b6bc377109be13f1e22f4e0e3.pdf |
| work_keys_str_mv | AT xizhu salidrosideimpedesangiiinfusedmyocardialfibrosisbyactivatingthesirt1nrf2pathway AT zhenhai salidrosideimpedesangiiinfusedmyocardialfibrosisbyactivatingthesirt1nrf2pathway AT zhongpingning salidrosideimpedesangiiinfusedmyocardialfibrosisbyactivatingthesirt1nrf2pathway |