Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range.
Mosquitoes harbor a large diversity of eukaryotic viruses. Those viromes probably influence mosquito physiology and the transmission of human pathogens. Nevertheless, their ecology remains largely unstudied. Here, we address two key questions in virome ecology. First, we assessed the influence of mo...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0300915&type=printable |
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| author | Côme Morel Patricia Gil Antoni Exbrayat Etienne Loire Florian Charriat Baptiste Prepoint Celine Condachou Geoffrey Gimonneau Assane Gueye Fall Biram Biteye Momar Talla Seck Marc Eloit Serafin Gutierrez |
| author_facet | Côme Morel Patricia Gil Antoni Exbrayat Etienne Loire Florian Charriat Baptiste Prepoint Celine Condachou Geoffrey Gimonneau Assane Gueye Fall Biram Biteye Momar Talla Seck Marc Eloit Serafin Gutierrez |
| author_sort | Côme Morel |
| collection | DOAJ |
| description | Mosquitoes harbor a large diversity of eukaryotic viruses. Those viromes probably influence mosquito physiology and the transmission of human pathogens. Nevertheless, their ecology remains largely unstudied. Here, we address two key questions in virome ecology. First, we assessed the influence of mosquito species on virome taxonomic diversity and relative abundance. Contrary to most previous studies, the potential effect of the habitat was explicitly included. Thousands of individuals of Culex poicilipes and Culex tritaeniorhynchus, two vectors of viral diseases, were concomitantly sampled in three habitats over two years. A total of 95 viral taxa from 25 families were identified with meta-transcriptomics, with 75% of taxa shared by both mosquitoes. Viromes significantly differed by mosquito species but not by habitat. Differences were largely due to changes in relative abundance of shared taxa. Then, we studied the diversity of viruses with a broad host range. We searched for viral taxa shared by the two Culex species and Aedes vexans, another disease vector, present in one of the habitats. Twenty-six out of the 163 viral taxa were found in the three mosquitoes. These taxa encompassed 14 families. A database analysis supported broad host ranges for many of those viruses, as well as a widespread geographical distribution. Thus, the viromes of mosquitoes from the same genera mainly differed in the relative abundance of shared taxa, whereas differences in viral diversity dominated between mosquito genera. Whether this new model of virome diversity and structure applies to other mosquito communities remains to be determined. |
| format | Article |
| id | doaj-art-db359a7d2ef14595974c73cfa3b4e55d |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-db359a7d2ef14595974c73cfa3b4e55d2024-12-13T05:31:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01194e030091510.1371/journal.pone.0300915Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range.Côme MorelPatricia GilAntoni ExbrayatEtienne LoireFlorian CharriatBaptiste PrepointCeline CondachouGeoffrey GimonneauAssane Gueye FallBiram BiteyeMomar Talla SeckMarc EloitSerafin GutierrezMosquitoes harbor a large diversity of eukaryotic viruses. Those viromes probably influence mosquito physiology and the transmission of human pathogens. Nevertheless, their ecology remains largely unstudied. Here, we address two key questions in virome ecology. First, we assessed the influence of mosquito species on virome taxonomic diversity and relative abundance. Contrary to most previous studies, the potential effect of the habitat was explicitly included. Thousands of individuals of Culex poicilipes and Culex tritaeniorhynchus, two vectors of viral diseases, were concomitantly sampled in three habitats over two years. A total of 95 viral taxa from 25 families were identified with meta-transcriptomics, with 75% of taxa shared by both mosquitoes. Viromes significantly differed by mosquito species but not by habitat. Differences were largely due to changes in relative abundance of shared taxa. Then, we studied the diversity of viruses with a broad host range. We searched for viral taxa shared by the two Culex species and Aedes vexans, another disease vector, present in one of the habitats. Twenty-six out of the 163 viral taxa were found in the three mosquitoes. These taxa encompassed 14 families. A database analysis supported broad host ranges for many of those viruses, as well as a widespread geographical distribution. Thus, the viromes of mosquitoes from the same genera mainly differed in the relative abundance of shared taxa, whereas differences in viral diversity dominated between mosquito genera. Whether this new model of virome diversity and structure applies to other mosquito communities remains to be determined.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0300915&type=printable |
| spellingShingle | Côme Morel Patricia Gil Antoni Exbrayat Etienne Loire Florian Charriat Baptiste Prepoint Celine Condachou Geoffrey Gimonneau Assane Gueye Fall Biram Biteye Momar Talla Seck Marc Eloit Serafin Gutierrez Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range. PLoS ONE |
| title | Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range. |
| title_full | Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range. |
| title_fullStr | Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range. |
| title_full_unstemmed | Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range. |
| title_short | Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range. |
| title_sort | host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0300915&type=printable |
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