A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair
Abstract Background Treatment of peripheral nerve defects is a major concern in regenerative medicine. This study therefore aimed to explore the efficacy of a neural graft constructed using adipose mesenchymal stem cells (ADSC), acellular microtissues (MTs), and chitosan in the treatment of peripher...
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| Language: | English |
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BMC
2024-12-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-024-04093-5 |
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| author | Zhifa Zhang Molin Li Gang Cheng Peng Wang Chunhui Zhou Yang Liu Xiaofeng Duan Jing Wang Fang Xie Yaqiong Zhu Jianning Zhang |
| author_facet | Zhifa Zhang Molin Li Gang Cheng Peng Wang Chunhui Zhou Yang Liu Xiaofeng Duan Jing Wang Fang Xie Yaqiong Zhu Jianning Zhang |
| author_sort | Zhifa Zhang |
| collection | DOAJ |
| description | Abstract Background Treatment of peripheral nerve defects is a major concern in regenerative medicine. This study therefore aimed to explore the efficacy of a neural graft constructed using adipose mesenchymal stem cells (ADSC), acellular microtissues (MTs), and chitosan in the treatment of peripheral nerve defects. Methods Stem cell therapy with acellular MTs provided a suitable microenvironment for axonal regeneration, and compensated for the lack of repair cells in the neural ducts of male 8-week-old Sprague Dawley rats. Results In vitro, acellular MTs retained the intrinsic extracellular matrix and improved the narrow microstructure of acellular nerves, thereby enhancing cell functionality. In vivo neuroelectrophysiological studies, gait analysis, and sciatic nerve histology demonstrated the regenerative effects of active acellular MT. The Chitosan + Acellular-MT + ADSC group exhibited superior myelin sheath quality and improved neurological and motor function recovery. Conclusions Active acellular-MTs precellularized with ADSC hold promise as a safe and effective clinical treatment method for peripheral nerve defects. Graphical abstract |
| format | Article |
| id | doaj-art-db324a1029a84c2aa029e07952dd5e57 |
| institution | Kabale University |
| issn | 1757-6512 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj-art-db324a1029a84c2aa029e07952dd5e572025-01-05T12:10:18ZengBMCStem Cell Research & Therapy1757-65122024-12-0115111410.1186/s13287-024-04093-5A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repairZhifa Zhang0Molin Li1Gang Cheng2Peng Wang3Chunhui Zhou4Yang Liu5Xiaofeng Duan6Jing Wang7Fang Xie8Yaqiong Zhu9Jianning Zhang10Departments of Neurosurgery, The First Center of Chinese, PLA General HospitalDepartments of Ultrasound, Tianjin Medical University Cancer Institute & HospitalDepartments of Neurosurgery, The First Center of Chinese, PLA General HospitalDepartments of Neurosurgery, The First Center of Chinese, PLA General HospitalDepartments of Neurosurgery, Chinese PLA General HospitalDepartment of Urology, The Affiliated Hospital of Changchun University of Chinese MedicineDepartment of Anesthesiology, Beijing Chao-Yang Hospital, Capital Medical UniversityDepartment of Orthopedic, Maanshan General Hospital of Ranger-Duree HealthcareDepartments of Ultrasound, The First Center of Chinese, PLA General HospitalDepartments of Ultrasound, The First Center of Chinese, PLA General HospitalDepartments of Neurosurgery, The First Center of Chinese, PLA General HospitalAbstract Background Treatment of peripheral nerve defects is a major concern in regenerative medicine. This study therefore aimed to explore the efficacy of a neural graft constructed using adipose mesenchymal stem cells (ADSC), acellular microtissues (MTs), and chitosan in the treatment of peripheral nerve defects. Methods Stem cell therapy with acellular MTs provided a suitable microenvironment for axonal regeneration, and compensated for the lack of repair cells in the neural ducts of male 8-week-old Sprague Dawley rats. Results In vitro, acellular MTs retained the intrinsic extracellular matrix and improved the narrow microstructure of acellular nerves, thereby enhancing cell functionality. In vivo neuroelectrophysiological studies, gait analysis, and sciatic nerve histology demonstrated the regenerative effects of active acellular MT. The Chitosan + Acellular-MT + ADSC group exhibited superior myelin sheath quality and improved neurological and motor function recovery. Conclusions Active acellular-MTs precellularized with ADSC hold promise as a safe and effective clinical treatment method for peripheral nerve defects. Graphical abstracthttps://doi.org/10.1186/s13287-024-04093-5Peripheral nerve injuryMesenchymal stem cellsTissue repairBiomaterialsChitosanAcellular nerve |
| spellingShingle | Zhifa Zhang Molin Li Gang Cheng Peng Wang Chunhui Zhou Yang Liu Xiaofeng Duan Jing Wang Fang Xie Yaqiong Zhu Jianning Zhang A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair Stem Cell Research & Therapy Peripheral nerve injury Mesenchymal stem cells Tissue repair Biomaterials Chitosan Acellular nerve |
| title | A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair |
| title_full | A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair |
| title_fullStr | A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair |
| title_full_unstemmed | A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair |
| title_short | A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair |
| title_sort | chitosan acellular matrix based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair |
| topic | Peripheral nerve injury Mesenchymal stem cells Tissue repair Biomaterials Chitosan Acellular nerve |
| url | https://doi.org/10.1186/s13287-024-04093-5 |
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