The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogens
Trichoderma is widely utilized in the biological control of plant diseases. In this study, two antagonistic fungi were isolated from cadavers of Spodoptera litura and Mythimna separata, and were identified as Trichoderma afroharzianum and Trichoderma asperelloides. The mycelia of T. afroharzianum gr...
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Elsevier
2025-01-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964424002548 |
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| author | Futao Gao Zihao Wang Junhua Zhu Wenyan Li Xunyou Wang Xifa Yang Youwu Hao Shiheng An Xinming Yin Xiangyang Liu |
| author_facet | Futao Gao Zihao Wang Junhua Zhu Wenyan Li Xunyou Wang Xifa Yang Youwu Hao Shiheng An Xinming Yin Xiangyang Liu |
| author_sort | Futao Gao |
| collection | DOAJ |
| description | Trichoderma is widely utilized in the biological control of plant diseases. In this study, two antagonistic fungi were isolated from cadavers of Spodoptera litura and Mythimna separata, and were identified as Trichoderma afroharzianum and Trichoderma asperelloides. The mycelia of T. afroharzianum grew fastest on the PSA medium under all light conditions, with soluble starch and peptone serving as the optimal carbon and nitrogen sources, respectively. The optimal pH was 6.0 and the lethal temperature was 57°C. The mycelia of T. asperelloides grew fastest on the PDA medium under all light conditions, with maltose and peptone as the optimal carbon and nitrogen sources, respectively. The optimal pH was 5.0 and the lethal temperature was 58°C. The two strains demonstrated antifungal activity against four apple disease pathogens: Alternaria mali, Collectotrichum gloeosporioides, Botryosphaeria dothidea, and Valsa mali. Antagonistic culture results showed that T. afroharzianum achieved an inhibition percentage exceeding 55 % against all four pathogens, while T. asperelloides exhibited the highest antifungal activity against B. dothidea (67.66 %). The mechanisms for controlling apple pathogens were investigated by measuring the activities of Poly Galacturonase (PG) and β-glucosidase (β-GC). In B. dothidea, the activities of PG and β-GC decreased by 58.92 % and 79.54 % when treated with T. afroharzianum culture filtrates, and decreased by 49.28 % and 89.02 % when treated with T. asperelloides culture filtrates, respectively. These results provide valuable information to support the development of T. afroharzianum or T. asperelloides as microbial fungicides for apple diseases. |
| format | Article |
| id | doaj-art-db0f328d4c1d41ef8d63467736354fae |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-db0f328d4c1d41ef8d63467736354fae2025-01-09T06:13:13ZengElsevierBiological Control1049-96442025-01-01200105689The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogensFutao Gao0Zihao Wang1Junhua Zhu2Wenyan Li3Xunyou Wang4Xifa Yang5Youwu Hao6Shiheng An7Xinming Yin8Xiangyang Liu9College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Corresponding authors.College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory for Creation and Application of Novel Pesticides of Henan Province, Zhengzhou 450002, China; Nano Agro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Corresponding authors.Trichoderma is widely utilized in the biological control of plant diseases. In this study, two antagonistic fungi were isolated from cadavers of Spodoptera litura and Mythimna separata, and were identified as Trichoderma afroharzianum and Trichoderma asperelloides. The mycelia of T. afroharzianum grew fastest on the PSA medium under all light conditions, with soluble starch and peptone serving as the optimal carbon and nitrogen sources, respectively. The optimal pH was 6.0 and the lethal temperature was 57°C. The mycelia of T. asperelloides grew fastest on the PDA medium under all light conditions, with maltose and peptone as the optimal carbon and nitrogen sources, respectively. The optimal pH was 5.0 and the lethal temperature was 58°C. The two strains demonstrated antifungal activity against four apple disease pathogens: Alternaria mali, Collectotrichum gloeosporioides, Botryosphaeria dothidea, and Valsa mali. Antagonistic culture results showed that T. afroharzianum achieved an inhibition percentage exceeding 55 % against all four pathogens, while T. asperelloides exhibited the highest antifungal activity against B. dothidea (67.66 %). The mechanisms for controlling apple pathogens were investigated by measuring the activities of Poly Galacturonase (PG) and β-glucosidase (β-GC). In B. dothidea, the activities of PG and β-GC decreased by 58.92 % and 79.54 % when treated with T. afroharzianum culture filtrates, and decreased by 49.28 % and 89.02 % when treated with T. asperelloides culture filtrates, respectively. These results provide valuable information to support the development of T. afroharzianum or T. asperelloides as microbial fungicides for apple diseases.http://www.sciencedirect.com/science/article/pii/S1049964424002548TrichodermaBiological characteristicsAntifungal activityApple pathogensAntifungal mechanism |
| spellingShingle | Futao Gao Zihao Wang Junhua Zhu Wenyan Li Xunyou Wang Xifa Yang Youwu Hao Shiheng An Xinming Yin Xiangyang Liu The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogens Biological Control Trichoderma Biological characteristics Antifungal activity Apple pathogens Antifungal mechanism |
| title | The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogens |
| title_full | The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogens |
| title_fullStr | The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogens |
| title_full_unstemmed | The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogens |
| title_short | The characterization and antifungal activities of two new Trichoderma antagonistic fungi against four apple disease pathogens |
| title_sort | characterization and antifungal activities of two new trichoderma antagonistic fungi against four apple disease pathogens |
| topic | Trichoderma Biological characteristics Antifungal activity Apple pathogens Antifungal mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S1049964424002548 |
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