Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach
Curcuma longa is a medicinal plant renowned for its therapeutic properties and potential treatment of cancer. This study focused on the biosynthesis of diterpenoids in the rhizome and leaves of C. longa. The genes responsible for producing these medicinal compounds were analyzed using BLASTx, Gene O...
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| Language: | English |
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University of Brawijaya
2024-09-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3195 |
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| author | Hafizh Fadhullah Devit Purwoko Siti Zulaeha Nurul Fitri Hanifah Endah Dwi Hartuti Gemilang Rahmadara Anna Safarrida Rikania Reninta Evawati Evawati Irwan Roza Teuku Tajuddin |
| author_facet | Hafizh Fadhullah Devit Purwoko Siti Zulaeha Nurul Fitri Hanifah Endah Dwi Hartuti Gemilang Rahmadara Anna Safarrida Rikania Reninta Evawati Evawati Irwan Roza Teuku Tajuddin |
| author_sort | Hafizh Fadhullah |
| collection | DOAJ |
| description | Curcuma longa is a medicinal plant renowned for its therapeutic properties and potential treatment of cancer. This study focused on the biosynthesis of diterpenoids in the rhizome and leaves of C. longa. The genes responsible for producing these medicinal compounds were analyzed using BLASTx, Gene Ontology (GO) annotation, differential expression, and homology. The substantial dataset was obtained from the National Center for Biotechnology Information (NCBI), comprising 151,730,334 clean reads and 167,264 transcripts for the analysis. The results of the BLASTx analysis were as follows: NR yielded 65.93%, Swiss-Prot yielded 44.52%, and COG yielded 17.35%. Subsequently, GO annotation was performed using Blast2GO, resulting in an annotation rate of 56.79%. Differential expression analysis revealed a total of 636 genes that were significantly differentiated between the rhizome and leaves. The homology analysis resulted in 11 proteins associated with diterpenoid biosynthesis and nine proteins related to CYP450. Approximately three class I proteins were highly expressed in the rhizome. Additionally, seven CYP450 enzymes from the CYP71D and CYP726 subfamilies were identified; three were highly expressed in the rhizome. The expression patterns of these enzymes were similar to the aforementioned three class I diTPSs, indicating their potential involvement in macroditerpenoid biosynthesis in C. longa. These findings provide valuable genomic resources for future functional genomics research on C. longa, facilitating targeted efforts to enhance the production of bioactive compounds. |
| format | Article |
| id | doaj-art-dac79bbc80d9453a8a5f8c46f30e20e7 |
| institution | Kabale University |
| issn | 2087-5517 2527-4376 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj-art-dac79bbc80d9453a8a5f8c46f30e20e72024-12-03T01:30:28ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172527-43762024-09-01143509 – 536509 – 53610.11594/jtls.14.03.083140Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing ApproachHafizh Fadhullah0Devit Purwoko1Siti Zulaeha2Nurul Fitri Hanifah3Endah Dwi Hartuti4Gemilang Rahmadara5Anna Safarrida6Rikania Reninta7Evawati Evawati8Irwan Roza9Teuku Tajuddin10Biotechnology Graduate Program, Graduate School, Bogor Agricultural University (IPB University), Bogor 16680, IndonesiaResearch Centre for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Science and Technology Park of Soekarno, Cibinong, Bogor West Java 16911, IndonesiaResearch Centre for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Science and Technology Park of Soekarno, Cibinong, Bogor West Java 16911, IndonesiaResearch Centre for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Science and Technology Park of Soekarno, Cibinong, Bogor West Java 16911, IndonesiaResearch Center for Genetic Engineering, Research Organization for Life Sciences and Environment, BRIN, Bogor, West Java 16911, IndonesiaResearch Centre for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Science and Technology Park of Soekarno, Cibinong, Bogor West Java 16911, IndonesiaResearch Centre for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Science and Technology Park of Soekarno, Cibinong, Bogor West Java 16911, IndonesiaResearch Center for Genetic Engineering, Research Organization for Life Sciences and Environment, BRIN, Bogor, West Java 16911, IndonesiaFood Technology Study Program, Department of Agricultural Engineering, Politeknik Pertanian Negeri Payakumbuh, West Sumatra 26271, IndonesiaFood Technology Study Program, Department of Agricultural Product Technology, Politeknik Pertanian Negeri Payakumbuh, Payakumbuh 26271, IndonesiaResearch Centre for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Science and Technology Park of Soekarno, Cibinong, Bogor West Java 16911, IndonesiaCurcuma longa is a medicinal plant renowned for its therapeutic properties and potential treatment of cancer. This study focused on the biosynthesis of diterpenoids in the rhizome and leaves of C. longa. The genes responsible for producing these medicinal compounds were analyzed using BLASTx, Gene Ontology (GO) annotation, differential expression, and homology. The substantial dataset was obtained from the National Center for Biotechnology Information (NCBI), comprising 151,730,334 clean reads and 167,264 transcripts for the analysis. The results of the BLASTx analysis were as follows: NR yielded 65.93%, Swiss-Prot yielded 44.52%, and COG yielded 17.35%. Subsequently, GO annotation was performed using Blast2GO, resulting in an annotation rate of 56.79%. Differential expression analysis revealed a total of 636 genes that were significantly differentiated between the rhizome and leaves. The homology analysis resulted in 11 proteins associated with diterpenoid biosynthesis and nine proteins related to CYP450. Approximately three class I proteins were highly expressed in the rhizome. Additionally, seven CYP450 enzymes from the CYP71D and CYP726 subfamilies were identified; three were highly expressed in the rhizome. The expression patterns of these enzymes were similar to the aforementioned three class I diTPSs, indicating their potential involvement in macroditerpenoid biosynthesis in C. longa. These findings provide valuable genomic resources for future functional genomics research on C. longa, facilitating targeted efforts to enhance the production of bioactive compounds.https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3195curcuma longaditerpenoid biosynthesisgene expression |
| spellingShingle | Hafizh Fadhullah Devit Purwoko Siti Zulaeha Nurul Fitri Hanifah Endah Dwi Hartuti Gemilang Rahmadara Anna Safarrida Rikania Reninta Evawati Evawati Irwan Roza Teuku Tajuddin Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach Journal of Tropical Life Science curcuma longa diterpenoid biosynthesis gene expression |
| title | Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach |
| title_full | Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach |
| title_fullStr | Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach |
| title_full_unstemmed | Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach |
| title_short | Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach |
| title_sort | identification of gene candidates in diterpenoid biosynthesis of curcuma longa an mrna sequencing approach |
| topic | curcuma longa diterpenoid biosynthesis gene expression |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3195 |
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