Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruit
Abstract Background Fruit size is a crucial economic trait that impacts the quality of jujube (Ziziphus jujuba), however, research in this area remains limited. This study utilized two jujube cultivars with similar genetic backgrounds but differing fruit sizes to investigate the regulatory mechanism...
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BMC
2024-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-05997-3 |
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| author | Meiyu Li Siqian Jiao Pengqiang Yao Yuanzhe Xie Xiaolei Yang Huijin Zhang Hongju Yao Junchao liu Xiaoming Pang Wenhao Bo Shiping Cheng |
| author_facet | Meiyu Li Siqian Jiao Pengqiang Yao Yuanzhe Xie Xiaolei Yang Huijin Zhang Hongju Yao Junchao liu Xiaoming Pang Wenhao Bo Shiping Cheng |
| author_sort | Meiyu Li |
| collection | DOAJ |
| description | Abstract Background Fruit size is a crucial economic trait that impacts the quality of jujube (Ziziphus jujuba), however, research in this area remains limited. This study utilized two jujube cultivars with similar genetic backgrounds but differing fruit sizes to investigate the regulatory mechanisms affecting fruit size through cytological observations, transcriptome sequencing, and heterologous overexpression. Results The findings reveal that variations in mesocarp cell numbers during early fruit development significantly influence final fruit size. Comparative transcriptome sequencing identified 16,778 differentially expressed genes across five developmental stages. Co-expression network analysis identified 16 co-expression modules. KEGG enrichment analysis of these modules indicated the significant role of plant hormone-related pathways in fruit development. A detailed analysis of the ‘sky blue’ module revealed candidate genes associated with the regulation of mesocarp cells, leading to the construction of a regulatory network. Heterologous overexpression of the candidate gene ZjZOG in tomato confirmed its positive role in fruit size enhancement. Transcriptomic analysis of these overexpression lines further validated the regulatory network and identified reliable candidate genes for fruit size control. Conclusion This study provides valuable insights into the regulatory mechanisms of fruit size, which may facilitate the breeding of larger jujube cultivars. |
| format | Article |
| id | doaj-art-da8c51c656a44c71977f6395276dba93 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-da8c51c656a44c71977f6395276dba932024-12-29T12:15:12ZengBMCBMC Plant Biology1471-22292024-12-0124111310.1186/s12870-024-05997-3Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruitMeiyu Li0Siqian Jiao1Pengqiang Yao2Yuanzhe Xie3Xiaolei Yang4Huijin Zhang5Hongju Yao6Junchao liu7Xiaoming Pang8Wenhao Bo9Shiping Cheng10Henan Province Key Laboratory of Germplasm Innovation and Utilization of Eco- economic Woody Plant, Pingdingshan UniversityHenan Province Key Laboratory of Germplasm Innovation and Utilization of Eco- economic Woody Plant, Pingdingshan UniversityHenan Province Key Laboratory of Germplasm Innovation and Utilization of Eco- economic Woody Plant, Pingdingshan UniversityNational Tree Breeding Station for Platycladus orientalis in Jiaxian, Forest Farm of Jiaxian CountyNational Tree Breeding Station for Platycladus orientalis in Jiaxian, Forest Farm of Jiaxian CountyNational Tree Breeding Station for Platycladus orientalis in Jiaxian, Forest Farm of Jiaxian CountyNational Tree Breeding Station for Platycladus orientalis in Jiaxian, Forest Farm of Jiaxian CountyNational Tree Breeding Station for Platycladus orientalis in Jiaxian, Forest Farm of Jiaxian CountyState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry UniversityHenan Province Key Laboratory of Germplasm Innovation and Utilization of Eco- economic Woody Plant, Pingdingshan UniversityAbstract Background Fruit size is a crucial economic trait that impacts the quality of jujube (Ziziphus jujuba), however, research in this area remains limited. This study utilized two jujube cultivars with similar genetic backgrounds but differing fruit sizes to investigate the regulatory mechanisms affecting fruit size through cytological observations, transcriptome sequencing, and heterologous overexpression. Results The findings reveal that variations in mesocarp cell numbers during early fruit development significantly influence final fruit size. Comparative transcriptome sequencing identified 16,778 differentially expressed genes across five developmental stages. Co-expression network analysis identified 16 co-expression modules. KEGG enrichment analysis of these modules indicated the significant role of plant hormone-related pathways in fruit development. A detailed analysis of the ‘sky blue’ module revealed candidate genes associated with the regulation of mesocarp cells, leading to the construction of a regulatory network. Heterologous overexpression of the candidate gene ZjZOG in tomato confirmed its positive role in fruit size enhancement. Transcriptomic analysis of these overexpression lines further validated the regulatory network and identified reliable candidate genes for fruit size control. Conclusion This study provides valuable insights into the regulatory mechanisms of fruit size, which may facilitate the breeding of larger jujube cultivars.https://doi.org/10.1186/s12870-024-05997-3Ziziphus jujubaFruit sizeZjZOGTranscriptomics |
| spellingShingle | Meiyu Li Siqian Jiao Pengqiang Yao Yuanzhe Xie Xiaolei Yang Huijin Zhang Hongju Yao Junchao liu Xiaoming Pang Wenhao Bo Shiping Cheng Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruit BMC Plant Biology Ziziphus jujuba Fruit size ZjZOG Transcriptomics |
| title | Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruit |
| title_full | Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruit |
| title_fullStr | Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruit |
| title_full_unstemmed | Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruit |
| title_short | Comparative transcriptome analysis and heterologous overexpression indicate that the ZjZOG gene may positively regulate the size of jujube fruit |
| title_sort | comparative transcriptome analysis and heterologous overexpression indicate that the zjzog gene may positively regulate the size of jujube fruit |
| topic | Ziziphus jujuba Fruit size ZjZOG Transcriptomics |
| url | https://doi.org/10.1186/s12870-024-05997-3 |
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