Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration
We have developed a high-throughput, full field-of-view (FOV) spectroscopic single-molecule fluorescence instrument, overcoming distortion issues with gratings and prisms. Our calibration techniques ensure spectral uniformity across the whole FOV, and our two-step Gaussian fit method enables better...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2025-01-01
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| Series: | Applied Physics Express |
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| Online Access: | https://doi.org/10.35848/1882-0786/ada10b |
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| author | Deqi Yi Shangyuan Deng Chuang Tan Jie Ma |
| author_facet | Deqi Yi Shangyuan Deng Chuang Tan Jie Ma |
| author_sort | Deqi Yi |
| collection | DOAJ |
| description | We have developed a high-throughput, full field-of-view (FOV) spectroscopic single-molecule fluorescence instrument, overcoming distortion issues with gratings and prisms. Our calibration techniques ensure spectral uniformity across the whole FOV, and our two-step Gaussian fit method enables better than 2 nm precision in spectral peak determination for accurate molecule identification, making it a valuable tool for multi-color single-molecule detection and analysis. |
| format | Article |
| id | doaj-art-da81ef1794694ca5ad3770a7f6171591 |
| institution | Kabale University |
| issn | 1882-0786 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Applied Physics Express |
| spelling | doaj-art-da81ef1794694ca5ad3770a7f61715912025-01-14T16:11:13ZengIOP PublishingApplied Physics Express1882-07862025-01-0118101200310.35848/1882-0786/ada10bDevelopment of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibrationDeqi Yi0Shangyuan Deng1Chuang Tan2Jie Ma3https://orcid.org/0000-0002-7570-9554School of Physics, Sun Yat-sen University , Guangzhou 510275, People’s Republic of China; State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University , Guangzhou 510275, People’s Republic of ChinaSchool of Physics, Sun Yat-sen University , Guangzhou 510275, People’s Republic of China; State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University , Guangzhou 510275, People’s Republic of ChinaSchool of Physics, Sun Yat-sen University , Guangzhou 510275, People’s Republic of China; State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University , Guangzhou 510275, People’s Republic of ChinaSchool of Physics, Sun Yat-sen University , Guangzhou 510275, People’s Republic of China; State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University , Guangzhou 510275, People’s Republic of ChinaWe have developed a high-throughput, full field-of-view (FOV) spectroscopic single-molecule fluorescence instrument, overcoming distortion issues with gratings and prisms. Our calibration techniques ensure spectral uniformity across the whole FOV, and our two-step Gaussian fit method enables better than 2 nm precision in spectral peak determination for accurate molecule identification, making it a valuable tool for multi-color single-molecule detection and analysis.https://doi.org/10.35848/1882-0786/ada10bsingle-molecule fluorescence spectroscopyspectrometer calibrationdistortion correctionsingle-molecule spectral analysis |
| spellingShingle | Deqi Yi Shangyuan Deng Chuang Tan Jie Ma Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration Applied Physics Express single-molecule fluorescence spectroscopy spectrometer calibration distortion correction single-molecule spectral analysis |
| title | Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration |
| title_full | Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration |
| title_fullStr | Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration |
| title_full_unstemmed | Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration |
| title_short | Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration |
| title_sort | development of a full field of view spectroscopic single molecule fluorescence instrument with improved methods for calibration |
| topic | single-molecule fluorescence spectroscopy spectrometer calibration distortion correction single-molecule spectral analysis |
| url | https://doi.org/10.35848/1882-0786/ada10b |
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