APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL
Glucomannan purification methods affect the properties of mannan and have influence to the scope of glucomannan applications. Combination between ethanol solution and thermal treatment is commonly method applied to purify glucomannan obtained from Amorphophallus sp. However, 2-propanol was reported...
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2016-04-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201601&vol=1&aid=4360 |
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| author | DYAH H. WARDHANI FATONI NUGROHO M. MUSLIHUDIN NITA ARYANTI |
| author_facet | DYAH H. WARDHANI FATONI NUGROHO M. MUSLIHUDIN NITA ARYANTI |
| author_sort | DYAH H. WARDHANI |
| collection | DOAJ |
| description | Glucomannan purification methods affect the properties of mannan and have influence to the scope of glucomannan applications. Combination between ethanol solution and thermal treatment is commonly method applied to purify glucomannan obtained from Amorphophallus sp. However, 2-propanol was reported to be more effective in removing glucomannan impurities including the starch and the carotenes. The objective of this research was to study the effect of 2-propanol concentration, temperature and time as well as their interaction on purification of glucomannan obtained from Amorphophallus oncophyllus by using response surface methods. The relevant parameters (glucomannan content, starch content, degree of whiteness, yield) were investigated in order to establish mathematical model. The results showed that the linear models were reliable to predict the responses (R2 ≥ 0.926). Temperature was a significant variable for the all responses. This purification method improved the absorbance of the functional groups at ~2900 cm-1 of stretching of C-H vibration and ~1730 cm-1 of acetyl group which is responsible on glucomannan solubility. However, this method reduced the absorbance of the functional groups at ~1650, ~1070, ~1020 and 900 - 800 cm-1 of absorbed water or protein, C-O alcohol and -glucosidic and -mannosidic linkages, respectively. The morphology observation revealed that this purification method achieves separation and removal of impurities which encapsulated the glucomannan and subsequently released the glucomannan granules. The highest content of glucomannan obtained in this study was 72.30 % when 90 % 2-propanol at 75 °C for 180 min was used for purification. |
| format | Article |
| id | doaj-art-da7ffeb8dbde48adbd6349b9dd569f2c |
| institution | DOAJ |
| issn | 1582-540X 1582-540X |
| language | English |
| publishDate | 2016-04-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-da7ffeb8dbde48adbd6349b9dd569f2c2025-08-20T03:09:54ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2016-04-01171063074APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOLDYAH H. WARDHANI0FATONI NUGROHO1M. MUSLIHUDIN2NITA ARYANTI3University of Diponegoro, Faculty of Engineering, Department of Chemical Engineering, Jl. Prof. Sudarto SH, Tembalang, Semarang, Indonesia University of Diponegoro, Faculty of Engineering, Department of Chemical Engineering, Jl. Prof. Sudarto SH, Tembalang, Semarang, Indonesia University of Diponegoro, Faculty of Engineering, Department of Chemical Engineering, Jl. Prof. Sudarto SH, Tembalang, Semarang, Indonesia University of Diponegoro, Faculty of Engineering, Department of Chemical Engineering, Jl. Prof. Sudarto SH, Tembalang, Semarang, Indonesia Glucomannan purification methods affect the properties of mannan and have influence to the scope of glucomannan applications. Combination between ethanol solution and thermal treatment is commonly method applied to purify glucomannan obtained from Amorphophallus sp. However, 2-propanol was reported to be more effective in removing glucomannan impurities including the starch and the carotenes. The objective of this research was to study the effect of 2-propanol concentration, temperature and time as well as their interaction on purification of glucomannan obtained from Amorphophallus oncophyllus by using response surface methods. The relevant parameters (glucomannan content, starch content, degree of whiteness, yield) were investigated in order to establish mathematical model. The results showed that the linear models were reliable to predict the responses (R2 ≥ 0.926). Temperature was a significant variable for the all responses. This purification method improved the absorbance of the functional groups at ~2900 cm-1 of stretching of C-H vibration and ~1730 cm-1 of acetyl group which is responsible on glucomannan solubility. However, this method reduced the absorbance of the functional groups at ~1650, ~1070, ~1020 and 900 - 800 cm-1 of absorbed water or protein, C-O alcohol and -glucosidic and -mannosidic linkages, respectively. The morphology observation revealed that this purification method achieves separation and removal of impurities which encapsulated the glucomannan and subsequently released the glucomannan granules. The highest content of glucomannan obtained in this study was 72.30 % when 90 % 2-propanol at 75 °C for 180 min was used for purification.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201601&vol=1&aid=4360Amorphophallus oncophyllusextractionglucomannan2-propanolpurificationresponse surface methods |
| spellingShingle | DYAH H. WARDHANI FATONI NUGROHO M. MUSLIHUDIN NITA ARYANTI APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry Amorphophallus oncophyllus extraction glucomannan 2-propanol purification response surface methods |
| title | APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL |
| title_full | APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL |
| title_fullStr | APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL |
| title_full_unstemmed | APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL |
| title_short | APPLICATION OF RESPONSE SURFACE METHOD ON PURIFICATION OF GLUCOMANNAN FROM AMORPHOPHALLUS ONCOPHYLLUS BY USING 2-PROPANOL |
| title_sort | application of response surface method on purification of glucomannan from amorphophallus oncophyllus by using 2 propanol |
| topic | Amorphophallus oncophyllus extraction glucomannan 2-propanol purification response surface methods |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201601&vol=1&aid=4360 |
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