Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study

Abstract Background Growing data indicated that individuals diagnosed with major depressive disorder (MDD) had widespread inflammation, oxidative stress, and metabolic dysregulation. The objective of our study was to detect alterations in lipids of individuals with MDD, with the ultimate aim of deve...

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Main Authors: Lei He, Na Duan, Chong Wang, Ru Shan, Jing Li, Lin Wang, Qiuli Liu, Junwei Tao, Li Liu, Xiaoliang Ma, Bing Cao
Format: Article
Language:English
Published: BMC 2025-08-01
Series:BMC Psychiatry
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Online Access:https://doi.org/10.1186/s12888-025-07191-7
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author Lei He
Na Duan
Chong Wang
Ru Shan
Jing Li
Lin Wang
Qiuli Liu
Junwei Tao
Li Liu
Xiaoliang Ma
Bing Cao
author_facet Lei He
Na Duan
Chong Wang
Ru Shan
Jing Li
Lin Wang
Qiuli Liu
Junwei Tao
Li Liu
Xiaoliang Ma
Bing Cao
author_sort Lei He
collection DOAJ
description Abstract Background Growing data indicated that individuals diagnosed with major depressive disorder (MDD) had widespread inflammation, oxidative stress, and metabolic dysregulation. The objective of our study was to detect alterations in lipids of individuals with MDD, with the ultimate aim of developing potential biomarkers that may contribute to the diagnosis and treatment of MDD. Methods The current study was a single-center cross-sectional case-control design. Serum samples were obtained from 107 individuals diagnosed with MDD and 97 healthy controls (HC) aged 18 to 60 years. Lipidomics analysis was performed using an Ultimate 3000 UHPLC system coupled with a Q-Exactive HF MS platform. All data were processed using the specialized online software Metaboanalyst 5.0. Results Based on the filtering criteria of false discovery rate (FDR) -adjusted P < 0.05, variable importance in projection (VIP) > 1.5, and fold change (FC) > 2.0 or < 0.5, a total of 40 lipids were identified as significantly different. In patients with MDD, we observed an increase in 11 types of oxidized fatty acids (OxFAs) and a decrease in 5 types of OxFAs. Additionally, we found that 8 types of acyl-carnitines (CARs) decreased, primarily comprising singular carbon chain structures, while 3 types of CARs increased, all with numerical carbon chain patterns. Lipid profiles yield a high area under the receiver operating characteristic (ROC) curve for differentiating MDD, with the most prominent ROC ranking being mostly attributed to OxFAs. Conclusions Our research found significant variations in lipid levels, specifically increased OxFAs and decreased CARs, in individuals with MDD compared to HCs. Supplementation with PUFAs and acyl-carnitines warrants further investigation as a potential strategy for the management of MDD. Nevertheless, further investigation is necessary, and exercise prudence is required when examining and implementing their forms and proportions.
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spelling doaj-art-da6f3b6a1c0740a98c7a3cd664ac49d32025-08-20T04:02:54ZengBMCBMC Psychiatry1471-244X2025-08-0125111010.1186/s12888-025-07191-7Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control studyLei He0Na Duan1Chong Wang2Ru Shan3Jing Li4Lin Wang5Qiuli Liu6Junwei Tao7Li Liu8Xiaoliang Ma9Bing Cao10The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)The Affiliated Brain Hospital of Zhengzhou University(The Second People’s Hospital of Zhumadian)Key Laboratory of Cognition and Personality, Faculty of Psychology, Ministry of Education, Southwest UniversityAbstract Background Growing data indicated that individuals diagnosed with major depressive disorder (MDD) had widespread inflammation, oxidative stress, and metabolic dysregulation. The objective of our study was to detect alterations in lipids of individuals with MDD, with the ultimate aim of developing potential biomarkers that may contribute to the diagnosis and treatment of MDD. Methods The current study was a single-center cross-sectional case-control design. Serum samples were obtained from 107 individuals diagnosed with MDD and 97 healthy controls (HC) aged 18 to 60 years. Lipidomics analysis was performed using an Ultimate 3000 UHPLC system coupled with a Q-Exactive HF MS platform. All data were processed using the specialized online software Metaboanalyst 5.0. Results Based on the filtering criteria of false discovery rate (FDR) -adjusted P < 0.05, variable importance in projection (VIP) > 1.5, and fold change (FC) > 2.0 or < 0.5, a total of 40 lipids were identified as significantly different. In patients with MDD, we observed an increase in 11 types of oxidized fatty acids (OxFAs) and a decrease in 5 types of OxFAs. Additionally, we found that 8 types of acyl-carnitines (CARs) decreased, primarily comprising singular carbon chain structures, while 3 types of CARs increased, all with numerical carbon chain patterns. Lipid profiles yield a high area under the receiver operating characteristic (ROC) curve for differentiating MDD, with the most prominent ROC ranking being mostly attributed to OxFAs. Conclusions Our research found significant variations in lipid levels, specifically increased OxFAs and decreased CARs, in individuals with MDD compared to HCs. Supplementation with PUFAs and acyl-carnitines warrants further investigation as a potential strategy for the management of MDD. Nevertheless, further investigation is necessary, and exercise prudence is required when examining and implementing their forms and proportions.https://doi.org/10.1186/s12888-025-07191-7Acyl-carnitineFatty acidOxidizedLipidsMajor depressive disorder
spellingShingle Lei He
Na Duan
Chong Wang
Ru Shan
Jing Li
Lin Wang
Qiuli Liu
Junwei Tao
Li Liu
Xiaoliang Ma
Bing Cao
Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study
BMC Psychiatry
Acyl-carnitine
Fatty acid
Oxidized
Lipids
Major depressive disorder
title Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study
title_full Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study
title_fullStr Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study
title_full_unstemmed Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study
title_short Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study
title_sort lipidomic analyses reveal the dysregulation of oxidized fatty acids oxfas and acyl carnitines cars in major depressive disorder a case control study
topic Acyl-carnitine
Fatty acid
Oxidized
Lipids
Major depressive disorder
url https://doi.org/10.1186/s12888-025-07191-7
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