Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice
In this study, RAW 264.7 cells and immunosuppressive models were used to investigate the immunostimulatory activity of tilapia-head chondroitin sulfate (CS) through in vitro and in vivo experiments. The results showed that tilapia-head chondroitin sulfate (CS) could induce the produce of NO, IL-1β,...
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The editorial department of Science and Technology of Food Industry
2024-12-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023120214 |
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| author | Houpei HUANG Yuan YUAN Zhuo WANG Jing CHEN Bingbing SONG Saiyi ZHONG |
| author_facet | Houpei HUANG Yuan YUAN Zhuo WANG Jing CHEN Bingbing SONG Saiyi ZHONG |
| author_sort | Houpei HUANG |
| collection | DOAJ |
| description | In this study, RAW 264.7 cells and immunosuppressive models were used to investigate the immunostimulatory activity of tilapia-head chondroitin sulfate (CS) through in vitro and in vivo experiments. The results showed that tilapia-head chondroitin sulfate (CS) could induce the produce of NO, IL-1β, IL-6, IL-10 and TNF-α in RAW 264.7 cells. Cyclophosphamide (CTX) induced thymic atrophy in mice (thymic index 0.11%±0.01%), while thymic index was significantly up-regulated by low-dose CS (thymic index (0.17%±0.03%) and high-dose CS (thymic index (0.18%±0.02%) interventions (P<0.05). Meanwhile, CS significantly increased spleen index in CTX-induced mice (P<0.05), promoted villus length and V/C value, with high-dose CS significantly increased villus length (330.92±21.55) μm (P<0.05). Furthermore, CS enhanced the secretion of serum sIgA in CTX-induced mice, indicating that CS could improve intestinal barrier damage and immune function in immunosuppressive mice. Therefore, this study provides a theoretical basis for the intestinal mucosal immune regulation of tilapia chondroitin sulfate, and suggests that tilapia-head chondroitin sulfate (CS) is a potential immunoregulatory polysaccharide that could replace shark chondroitin sulfate, potentially serving as a promising material for functional foods aimed at intervening in immune-related diseases. |
| format | Article |
| id | doaj-art-da40e1a1ef414b238df5e62ff01c61da |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-da40e1a1ef414b238df5e62ff01c61da2024-11-22T07:28:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-12-01452338138710.13386/j.issn1002-0306.20231202142023120214-23Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and MiceHoupei HUANG0Yuan YUAN1Zhuo WANG2Jing CHEN3Bingbing SONG4Saiyi ZHONG5Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaIn this study, RAW 264.7 cells and immunosuppressive models were used to investigate the immunostimulatory activity of tilapia-head chondroitin sulfate (CS) through in vitro and in vivo experiments. The results showed that tilapia-head chondroitin sulfate (CS) could induce the produce of NO, IL-1β, IL-6, IL-10 and TNF-α in RAW 264.7 cells. Cyclophosphamide (CTX) induced thymic atrophy in mice (thymic index 0.11%±0.01%), while thymic index was significantly up-regulated by low-dose CS (thymic index (0.17%±0.03%) and high-dose CS (thymic index (0.18%±0.02%) interventions (P<0.05). Meanwhile, CS significantly increased spleen index in CTX-induced mice (P<0.05), promoted villus length and V/C value, with high-dose CS significantly increased villus length (330.92±21.55) μm (P<0.05). Furthermore, CS enhanced the secretion of serum sIgA in CTX-induced mice, indicating that CS could improve intestinal barrier damage and immune function in immunosuppressive mice. Therefore, this study provides a theoretical basis for the intestinal mucosal immune regulation of tilapia chondroitin sulfate, and suggests that tilapia-head chondroitin sulfate (CS) is a potential immunoregulatory polysaccharide that could replace shark chondroitin sulfate, potentially serving as a promising material for functional foods aimed at intervening in immune-related diseases.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023120214tilapiachondroitin sulfateimmunostimulatory activityimmunosuppressionintestinal barrierraw 264.7 |
| spellingShingle | Houpei HUANG Yuan YUAN Zhuo WANG Jing CHEN Bingbing SONG Saiyi ZHONG Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice Shipin gongye ke-ji tilapia chondroitin sulfate immunostimulatory activity immunosuppression intestinal barrier raw 264.7 |
| title | Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice |
| title_full | Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice |
| title_fullStr | Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice |
| title_full_unstemmed | Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice |
| title_short | Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice |
| title_sort | immunostimulatory activity of tilapia head chondroitin sulfate in raw 264 7 cells and mice |
| topic | tilapia chondroitin sulfate immunostimulatory activity immunosuppression intestinal barrier raw 264.7 |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023120214 |
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