Determination of Intestinal Enzyme Activities During Infancy Period
Introduction: Intestinal enzyme activities are indirect indicators that reflect the existence and metabolic activity of bacteria living in the intestinal flora. The purpose of the study was to measure fecal beta (β)-glucuronidase, β-glucosidase and urease enzyme activities and to determine the facto...
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Galenos Publishing House
2016-08-01
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| Series: | Güncel Pediatri |
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| Online Access: | http://www.guncelpediatri.com/article_10738/Determination-Of-Intestinal-Enzyme-Activities-During-Infancy-Period |
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| author | Emel Örün Songül Yalçın İncilay Lay Arzu Dursun Asuman Özkara |
| author_facet | Emel Örün Songül Yalçın İncilay Lay Arzu Dursun Asuman Özkara |
| author_sort | Emel Örün |
| collection | DOAJ |
| description | Introduction: Intestinal enzyme activities are indirect indicators that reflect the existence and metabolic activity of bacteria living in the intestinal flora. The purpose of the study was to measure fecal beta (β)-glucuronidase, β-glucosidase and urease enzyme activities and to determine the factors that affect levels in 6 week old and 8 month old babies.
Materials and Methods: The study comprised 100 healthy infants at 6 weeks of age. Feces samples were collected from all infants. However, 17 of the feces samples were not included due to the lack of particles in the feces. The same samples were also taken from 35 infants at 8 months of age. Twenty-five of the infants had given feces samples at both 6 weeks and 8 months of age. Urease, β-glucuronidase and β-glucosidase enzyme activities (nmol/min-1/mg-protein-1) were measured.
Results: In repeated measures, the levels of β-glucuronidase and urease declined over time and β-glucosidase levels increased. At 8 months of age, higher β-glucuronidase levels were obtained in premature infants. At 6 weeks of age, lower levels of urease were measured in babies who were started breastfeeding at the first hour of life and were bottle-fed. Exclusive breastfeeding had no influence on the intestinal enzyme activities.
Conclusions: In early infancy period when microflora is structured, intestinal enzyme activities are important that show indirectly functionality of the microflora. However, it is difficult to highlight what affects the levels of intestinal enzymes because activities vary according to the age. |
| format | Article |
| id | doaj-art-da2d8c05f3d04d76af48a19d2bf64a88 |
| institution | Kabale University |
| issn | 1304-9054 |
| language | English |
| publishDate | 2016-08-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Güncel Pediatri |
| spelling | doaj-art-da2d8c05f3d04d76af48a19d2bf64a882025-01-02T03:43:56ZengGalenos Publishing HouseGüncel Pediatri1304-90542016-08-01142535910.4274/jcp.43434Determination of Intestinal Enzyme Activities During Infancy PeriodEmel Örün0Songül Yalçın1İncilay Lay2Arzu Dursun3Asuman Özkara4Turgut Özal Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, Ankara, TürkiyeHacettepe Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, Sosyal Pediatri Ünitesi, Ankara, TürkiyeHacettepe Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Ankara, TürkiyeDr. Sami Ulus Kadın Doğum, Çocuk Sağlığı ve Hastalıkları Eğitim ve Araştırma Hastanesi, Çocuk Sağlığı ve Hastalıkları Kliniği, Ankara, TürkiyeHacettepe Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Ankara, TürkiyeIntroduction: Intestinal enzyme activities are indirect indicators that reflect the existence and metabolic activity of bacteria living in the intestinal flora. The purpose of the study was to measure fecal beta (β)-glucuronidase, β-glucosidase and urease enzyme activities and to determine the factors that affect levels in 6 week old and 8 month old babies. Materials and Methods: The study comprised 100 healthy infants at 6 weeks of age. Feces samples were collected from all infants. However, 17 of the feces samples were not included due to the lack of particles in the feces. The same samples were also taken from 35 infants at 8 months of age. Twenty-five of the infants had given feces samples at both 6 weeks and 8 months of age. Urease, β-glucuronidase and β-glucosidase enzyme activities (nmol/min-1/mg-protein-1) were measured. Results: In repeated measures, the levels of β-glucuronidase and urease declined over time and β-glucosidase levels increased. At 8 months of age, higher β-glucuronidase levels were obtained in premature infants. At 6 weeks of age, lower levels of urease were measured in babies who were started breastfeeding at the first hour of life and were bottle-fed. Exclusive breastfeeding had no influence on the intestinal enzyme activities. Conclusions: In early infancy period when microflora is structured, intestinal enzyme activities are important that show indirectly functionality of the microflora. However, it is difficult to highlight what affects the levels of intestinal enzymes because activities vary according to the age.http://www.guncelpediatri.com/article_10738/Determination-Of-Intestinal-Enzyme-Activities-During-Infancy-PeriodIntestinal enzyme activityureasebeta- glucuronidase |
| spellingShingle | Emel Örün Songül Yalçın İncilay Lay Arzu Dursun Asuman Özkara Determination of Intestinal Enzyme Activities During Infancy Period Güncel Pediatri Intestinal enzyme activity urease beta- glucuronidase |
| title | Determination of Intestinal Enzyme Activities During Infancy Period |
| title_full | Determination of Intestinal Enzyme Activities During Infancy Period |
| title_fullStr | Determination of Intestinal Enzyme Activities During Infancy Period |
| title_full_unstemmed | Determination of Intestinal Enzyme Activities During Infancy Period |
| title_short | Determination of Intestinal Enzyme Activities During Infancy Period |
| title_sort | determination of intestinal enzyme activities during infancy period |
| topic | Intestinal enzyme activity urease beta- glucuronidase |
| url | http://www.guncelpediatri.com/article_10738/Determination-Of-Intestinal-Enzyme-Activities-During-Infancy-Period |
| work_keys_str_mv | AT emelorun determinationofintestinalenzymeactivitiesduringinfancyperiod AT songulyalcın determinationofintestinalenzymeactivitiesduringinfancyperiod AT incilaylay determinationofintestinalenzymeactivitiesduringinfancyperiod AT arzudursun determinationofintestinalenzymeactivitiesduringinfancyperiod AT asumanozkara determinationofintestinalenzymeactivitiesduringinfancyperiod |