Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm
Coronary artery vasospasm (CAV) is characterized by transient constriction of epicardial coronary arteries leading to angina. Its disease mechanisms are multifactorial but has centered mostly on endothelial dysfunction and smooth muscle hyperreactivity. To facilitate the investigation of these mecha...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124003428 |
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| author | Ines Ross Tacco Joseph Olshausen Tse Yuan Chan Naima Turbes Ming-Yow Hung Chi-Tai Yeh Patricia K. Nguyen Karim Sallam Nazish Sayed Ian Y. Chen |
| author_facet | Ines Ross Tacco Joseph Olshausen Tse Yuan Chan Naima Turbes Ming-Yow Hung Chi-Tai Yeh Patricia K. Nguyen Karim Sallam Nazish Sayed Ian Y. Chen |
| author_sort | Ines Ross Tacco |
| collection | DOAJ |
| description | Coronary artery vasospasm (CAV) is characterized by transient constriction of epicardial coronary arteries leading to angina. Its disease mechanisms are multifactorial but has centered mostly on endothelial dysfunction and smooth muscle hyperreactivity. To facilitate the investigation of these mechanisms in cell culture, we generated and characterized three induced pluripotent stem cell (iPSC) lines from patients with CAV. These lines demonstrated normal morphology and karyotypes, robust expression of pluripotency markers, and ability for tri-lineage differentiation. Further differentiation of these cell lines into endothelial and smooth muscle cells will allow mechanistic investigation of their relative contributions to CAV in cell culture. |
| format | Article |
| id | doaj-art-d9abbca3f54c41fc824d28254b4bd653 |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-d9abbca3f54c41fc824d28254b4bd6532025-01-13T04:18:43ZengElsevierStem Cell Research1873-50612025-02-0182103644Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasmInes Ross Tacco0Joseph Olshausen1Tse Yuan Chan2Naima Turbes3Ming-Yow Hung4Chi-Tai Yeh5Patricia K. Nguyen6Karim Sallam7Nazish Sayed8Ian Y. Chen9Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USACardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USACardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University, Stanford, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USADivision of Cardiology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Cardiology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Taipei Heart Institute, Taipei Medical University, Taipei, Taiwan; Department of Medical Research & Education, Taipei Medical University–Shuang Ho Hospital, New Taipei City, TaiwanDivision of Cardiology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Cardiology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Taipei Heart Institute, Taipei Medical University, Taipei, Taiwan; Department of Medical Research & Education, Taipei Medical University–Shuang Ho Hospital, New Taipei City, TaiwanCardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USACardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University, Stanford, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USACardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA; Corresponding author at: 3801 Miranda Ave, Palo Alto, CA 94304, USA. (I.Y. Chen).Coronary artery vasospasm (CAV) is characterized by transient constriction of epicardial coronary arteries leading to angina. Its disease mechanisms are multifactorial but has centered mostly on endothelial dysfunction and smooth muscle hyperreactivity. To facilitate the investigation of these mechanisms in cell culture, we generated and characterized three induced pluripotent stem cell (iPSC) lines from patients with CAV. These lines demonstrated normal morphology and karyotypes, robust expression of pluripotency markers, and ability for tri-lineage differentiation. Further differentiation of these cell lines into endothelial and smooth muscle cells will allow mechanistic investigation of their relative contributions to CAV in cell culture.http://www.sciencedirect.com/science/article/pii/S1873506124003428 |
| spellingShingle | Ines Ross Tacco Joseph Olshausen Tse Yuan Chan Naima Turbes Ming-Yow Hung Chi-Tai Yeh Patricia K. Nguyen Karim Sallam Nazish Sayed Ian Y. Chen Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm Stem Cell Research |
| title | Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm |
| title_full | Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm |
| title_fullStr | Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm |
| title_full_unstemmed | Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm |
| title_short | Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm |
| title_sort | generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124003428 |
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