TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A
Abstract Background Tongue squamous cell carcinoma (TSCC) is one of the most common malignant tumors with high mortality and poor prognosis. Its incidence rate is increasing gradually. Tumor necrosis factor receptor-associated factor interacting protein (TRAIP), as a factor related to several tumors...
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BMC
2024-12-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-024-13130-8 |
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| author | Litong Liu Ping Wang Cheng Guo Li Song Lifang Chen Hongbing Qi Yangyang Zheng Xiaoming Xing Chengqin Wang |
| author_facet | Litong Liu Ping Wang Cheng Guo Li Song Lifang Chen Hongbing Qi Yangyang Zheng Xiaoming Xing Chengqin Wang |
| author_sort | Litong Liu |
| collection | DOAJ |
| description | Abstract Background Tongue squamous cell carcinoma (TSCC) is one of the most common malignant tumors with high mortality and poor prognosis. Its incidence rate is increasing gradually. Tumor necrosis factor receptor-associated factor interacting protein (TRAIP), as a factor related to several tumors, reveals that its gene expression is different between normal tissue and primary tumor of head and neck squamous cell carcinoma using bioinformatics analysis. Method In our study, TCGA database, immunohistochemistry, proliferation assay, colony formation, wound healing assay, Transwell, cell cycle analysis and tumor xenografts model were used to determine the expression and functions of TRAIP in TSCC. Result We found that TRAIP may promote the proliferation, migration and invasion of TSCC. Furthermore, the results of bioinformatics analysis, mass spectrometry and co-immunoprecipitation suggested that DDX39A may be a TRAIP interacting protein. DDX39A has been proven to be an oncogene in several tumors, which may have an important effect on cell proliferation and metastasis in multiple tumors. In addition, the high expression of DDX39A implies the poor prognosis of patients. Our study demonstrated that TRAIP probably interact with DDX39A to regulate cell progression through epithelial-mesenchymal transition and Wnt/β-catenin pathway. In addition, we show that the necessary domain of DDX39A for the interaction between DDX39A and TRAIP region. Conclusion These results indicate that TRAIP is important in occurrence and development of TSCC and is expected to become the new promising therapeutic target. |
| format | Article |
| id | doaj-art-d997c24aef0045ee82d0e9d1fb4d1a90 |
| institution | Kabale University |
| issn | 1471-2407 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-d997c24aef0045ee82d0e9d1fb4d1a902024-12-08T12:33:43ZengBMCBMC Cancer1471-24072024-12-0124111410.1186/s12885-024-13130-8TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39ALitong Liu0Ping Wang1Cheng Guo2Li Song3Lifang Chen4Hongbing Qi5Yangyang Zheng6Xiaoming Xing7Chengqin Wang8Department of Pathology, School of Basic Medicine, Qingdao UniversityDepartment of Pathology, Linyi People’s HospitalDepartment of Pathology, School of Basic Medicine, Qingdao UniversityDepartment of Pathology, School of Basic Medicine, Qingdao UniversityDepartment of Pathology, the Affiliated Hospital of Qingdao UniversityDepartment of Pathology, the Affiliated Hospital of Qingdao UniversityDepartment of Pathology, School of Basic Medicine, Qingdao UniversityDepartment of Pathology, the Affiliated Hospital of Qingdao UniversityDepartment of Pathology, School of Basic Medicine, Qingdao UniversityAbstract Background Tongue squamous cell carcinoma (TSCC) is one of the most common malignant tumors with high mortality and poor prognosis. Its incidence rate is increasing gradually. Tumor necrosis factor receptor-associated factor interacting protein (TRAIP), as a factor related to several tumors, reveals that its gene expression is different between normal tissue and primary tumor of head and neck squamous cell carcinoma using bioinformatics analysis. Method In our study, TCGA database, immunohistochemistry, proliferation assay, colony formation, wound healing assay, Transwell, cell cycle analysis and tumor xenografts model were used to determine the expression and functions of TRAIP in TSCC. Result We found that TRAIP may promote the proliferation, migration and invasion of TSCC. Furthermore, the results of bioinformatics analysis, mass spectrometry and co-immunoprecipitation suggested that DDX39A may be a TRAIP interacting protein. DDX39A has been proven to be an oncogene in several tumors, which may have an important effect on cell proliferation and metastasis in multiple tumors. In addition, the high expression of DDX39A implies the poor prognosis of patients. Our study demonstrated that TRAIP probably interact with DDX39A to regulate cell progression through epithelial-mesenchymal transition and Wnt/β-catenin pathway. In addition, we show that the necessary domain of DDX39A for the interaction between DDX39A and TRAIP region. Conclusion These results indicate that TRAIP is important in occurrence and development of TSCC and is expected to become the new promising therapeutic target.https://doi.org/10.1186/s12885-024-13130-8TRAIPProgressionTongue squamous cell carcinomaWnt/β-catenin signalingEMTDDX39A |
| spellingShingle | Litong Liu Ping Wang Cheng Guo Li Song Lifang Chen Hongbing Qi Yangyang Zheng Xiaoming Xing Chengqin Wang TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A BMC Cancer TRAIP Progression Tongue squamous cell carcinoma Wnt/β-catenin signaling EMT DDX39A |
| title | TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A |
| title_full | TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A |
| title_fullStr | TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A |
| title_full_unstemmed | TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A |
| title_short | TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A |
| title_sort | traip enhances progression of tongue squamous cell carcinoma through emt and wnt β catenin signaling by interacting with ddx39a |
| topic | TRAIP Progression Tongue squamous cell carcinoma Wnt/β-catenin signaling EMT DDX39A |
| url | https://doi.org/10.1186/s12885-024-13130-8 |
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