The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in roots
Arbuscular mycorrhiza (AM) represents a symbiotic mutualistic association between most land plants and Glomeromycota fungi. AM fungi develops specialized intraradical and highly branched structures, called arbuscules, where bidirectional exchange of nutrients between plant and fungi partners occurs,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1472449/full |
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| author | Martín Ramos-Alvelo Nuria Molinero-Rosales María Isabel Tamayo-Navarrete Sanja Ćavar Zeljković Sanja Ćavar Zeljković Petr Tarkowski Petr Tarkowski José Manuel García-Garrido Tania Ho-Plágaro |
| author_facet | Martín Ramos-Alvelo Nuria Molinero-Rosales María Isabel Tamayo-Navarrete Sanja Ćavar Zeljković Sanja Ćavar Zeljković Petr Tarkowski Petr Tarkowski José Manuel García-Garrido Tania Ho-Plágaro |
| author_sort | Martín Ramos-Alvelo |
| collection | DOAJ |
| description | Arbuscular mycorrhiza (AM) represents a symbiotic mutualistic association between most land plants and Glomeromycota fungi. AM fungi develops specialized intraradical and highly branched structures, called arbuscules, where bidirectional exchange of nutrients between plant and fungi partners occurs, improving plant growth and fitness. Transcriptional reprogramming and hormonal regulation are necessary for the formation of the arbuscules. SlDLK2, a member of the third clade from the DWARF14 family of α, β-hydrolases closely related to the strigolactone receptor D14, is a negative regulator of arbuscule branching in tomato, but the underlying mechanisms are unknown. We explored the possible role of SlDLK2 on the regulation of hormonal balance. RNA-seq analysis was performed on roots from composite tomato plants overexpressing SlDLK2 and in control plants transformed with the empty vector. Analysis of transcriptomic data predicted that significantly repressed genes were enriched for genes related to hormone biosynthesis pathways, with a special relevance of carotenoid/apocarotenoid biosynthesis genes. Stable transgenic SlDLK2 overexpressing (OE) tomato lines were obtained, and hormone contents were analyzed in their roots and leaves. Interesting significant hormonal changes were found in roots of SlDLK2 OE lines with respect to the control lines, with a strong decrease on jasmonic acid and ABA. In addition, SlDLK2 OE roots showed a slight reduction in auxin contents and in one of the major strigolactones in tomato, solanacol. Overall, our results suggest that the negative regulation of AM symbiosis by SlDLK2 is associated with the repression of genes involved in the biosynthesis of AM-promoting hormones. |
| format | Article |
| id | doaj-art-d97e6ec3bd7b44218d5f2e181b779d2d |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-d97e6ec3bd7b44218d5f2e181b779d2d2024-12-11T06:45:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14724491472449The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in rootsMartín Ramos-Alvelo0Nuria Molinero-Rosales1María Isabel Tamayo-Navarrete2Sanja Ćavar Zeljković3Sanja Ćavar Zeljković4Petr Tarkowski5Petr Tarkowski6José Manuel García-Garrido7Tania Ho-Plágaro8Department of Soil and Plant Microbiology, Estación Experimental del Zaidín (EEZ), CSIC, Granada, SpainDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín (EEZ), CSIC, Granada, SpainDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín (EEZ), CSIC, Granada, SpainCzech Advanced Technology and Research Institute, Palacky University, Olomouc, CzechiaCentre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc, CzechiaCzech Advanced Technology and Research Institute, Palacky University, Olomouc, CzechiaCentre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc, CzechiaDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín (EEZ), CSIC, Granada, SpainDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín (EEZ), CSIC, Granada, SpainArbuscular mycorrhiza (AM) represents a symbiotic mutualistic association between most land plants and Glomeromycota fungi. AM fungi develops specialized intraradical and highly branched structures, called arbuscules, where bidirectional exchange of nutrients between plant and fungi partners occurs, improving plant growth and fitness. Transcriptional reprogramming and hormonal regulation are necessary for the formation of the arbuscules. SlDLK2, a member of the third clade from the DWARF14 family of α, β-hydrolases closely related to the strigolactone receptor D14, is a negative regulator of arbuscule branching in tomato, but the underlying mechanisms are unknown. We explored the possible role of SlDLK2 on the regulation of hormonal balance. RNA-seq analysis was performed on roots from composite tomato plants overexpressing SlDLK2 and in control plants transformed with the empty vector. Analysis of transcriptomic data predicted that significantly repressed genes were enriched for genes related to hormone biosynthesis pathways, with a special relevance of carotenoid/apocarotenoid biosynthesis genes. Stable transgenic SlDLK2 overexpressing (OE) tomato lines were obtained, and hormone contents were analyzed in their roots and leaves. Interesting significant hormonal changes were found in roots of SlDLK2 OE lines with respect to the control lines, with a strong decrease on jasmonic acid and ABA. In addition, SlDLK2 OE roots showed a slight reduction in auxin contents and in one of the major strigolactones in tomato, solanacol. Overall, our results suggest that the negative regulation of AM symbiosis by SlDLK2 is associated with the repression of genes involved in the biosynthesis of AM-promoting hormones.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1472449/fullarbuscular mycorrhizaplant hormonesDLK2transcriptomicstomato |
| spellingShingle | Martín Ramos-Alvelo Nuria Molinero-Rosales María Isabel Tamayo-Navarrete Sanja Ćavar Zeljković Sanja Ćavar Zeljković Petr Tarkowski Petr Tarkowski José Manuel García-Garrido Tania Ho-Plágaro The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in roots Frontiers in Microbiology arbuscular mycorrhiza plant hormones DLK2 transcriptomics tomato |
| title | The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in roots |
| title_full | The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in roots |
| title_fullStr | The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in roots |
| title_full_unstemmed | The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in roots |
| title_short | The SlDLK2 receptor, involved in the control of arbuscular mycorrhizal symbiosis, regulates hormonal balance in roots |
| title_sort | sldlk2 receptor involved in the control of arbuscular mycorrhizal symbiosis regulates hormonal balance in roots |
| topic | arbuscular mycorrhiza plant hormones DLK2 transcriptomics tomato |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1472449/full |
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