Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME

For the analysis of trimethoprim medication in its pure form and pharmaceutical formulations, precise and accurate microextraction techniques with UV-Vis measurement have been developed and confirmed. In this study, LLE and DLLME procedures were utilized for the separation, pre-concentration, and e...

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Main Authors: Araf I. Jabbar, Mohammed Z. Thani, Ali I. Khaleel
Format: Article
Language:English
Published: Tikrit University 2023-06-01
Series:Tikrit Journal of Pure Science
Subjects:
Online Access:https://tjpsj.org/index.php/tjps/article/view/1422
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author Araf I. Jabbar
Mohammed Z. Thani
Ali I. Khaleel
author_facet Araf I. Jabbar
Mohammed Z. Thani
Ali I. Khaleel
author_sort Araf I. Jabbar
collection DOAJ
description For the analysis of trimethoprim medication in its pure form and pharmaceutical formulations, precise and accurate microextraction techniques with UV-Vis measurement have been developed and confirmed. In this study, LLE and DLLME procedures were utilized for the separation, pre-concentration, and evaluation of trimethoprim in pure form and pharmaceutical preparations by UV-Vis spectroscopy at 390 nm. The method was validated and applied to the evaluation of trimethoprim in pharmaceutical formulations. Several experimental factors containing the type of dispersive and extraction solvents and their volumes, pH, temperature, and centrifuging time were carried out. Under the ideal conditions, the procedures were linear in the range of 2–45 and 1-10 mg/L, with a coefficient of determination (R2) of 0.9961 and 0.9992 for LLE and DLLME, respectively. The limit of detection (LOD) was 1.52 and 0.21 mg/L. Recovery of the target analyte in pharmaceutical formulations was 99.3%-104.9%.
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publisher Tikrit University
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spelling doaj-art-d9208c2a0f4c42d08fa9bb81d3b7c9b42025-08-20T03:50:20ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262023-06-0128310.25130/tjps.v28i3.1422Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLMEAraf I. JabbarMohammed Z. ThaniAli I. Khaleel For the analysis of trimethoprim medication in its pure form and pharmaceutical formulations, precise and accurate microextraction techniques with UV-Vis measurement have been developed and confirmed. In this study, LLE and DLLME procedures were utilized for the separation, pre-concentration, and evaluation of trimethoprim in pure form and pharmaceutical preparations by UV-Vis spectroscopy at 390 nm. The method was validated and applied to the evaluation of trimethoprim in pharmaceutical formulations. Several experimental factors containing the type of dispersive and extraction solvents and their volumes, pH, temperature, and centrifuging time were carried out. Under the ideal conditions, the procedures were linear in the range of 2–45 and 1-10 mg/L, with a coefficient of determination (R2) of 0.9961 and 0.9992 for LLE and DLLME, respectively. The limit of detection (LOD) was 1.52 and 0.21 mg/L. Recovery of the target analyte in pharmaceutical formulations was 99.3%-104.9%. https://tjpsj.org/index.php/tjps/article/view/1422TrimethoprimIon-pair complexliquid-liquid extraction (LLE)dispersive liquid-liquid extraction (DLLME)Microextraction
spellingShingle Araf I. Jabbar
Mohammed Z. Thani
Ali I. Khaleel
Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME
Tikrit Journal of Pure Science
Trimethoprim
Ion-pair complex
liquid-liquid extraction (LLE)
dispersive liquid-liquid extraction (DLLME)
Microextraction
title Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME
title_full Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME
title_fullStr Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME
title_full_unstemmed Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME
title_short Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME
title_sort ion pair complex of trimethoprim with alizarin yellow and its determination and extraction by lle and dllme
topic Trimethoprim
Ion-pair complex
liquid-liquid extraction (LLE)
dispersive liquid-liquid extraction (DLLME)
Microextraction
url https://tjpsj.org/index.php/tjps/article/view/1422
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