METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGY
Aim. The aim of the study was to develop the methodical approach to determine the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea, as well as to develop their technology in laboratory conditions. Materials and Methods. The following met...
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Danylo Halytsky Lviv National Medical University
2022-06-01
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| Series: | Acta Medica Leopoliensia |
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| Online Access: | https://amljournal.com/index.php/journal/article/view/273 |
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| author | Oksana Yezerska Nataliia Hudz Lesya Kobylinska Anna Filipska Vira Turkina Jan Brindza Piotr Pawel Wieczorek |
| author_facet | Oksana Yezerska Nataliia Hudz Lesya Kobylinska Anna Filipska Vira Turkina Jan Brindza Piotr Pawel Wieczorek |
| author_sort | Oksana Yezerska |
| collection | DOAJ |
| description | Aim. The aim of the study was to develop the methodical approach to determine the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea, as well as to develop their technology in laboratory conditions.
Materials and Methods. The following methods were used: analysis, synthesis, systematization, and comparison for processing of published scientific data on antioxidant activity; method for measuring the particle size of raw herbal materials; maceration and remaceration methods for obtaining the tested tinctures; DPPH test for the valuation of the antioxidant activity of the developed tinctures.
Results and Discussion.
The methodical approach to determining the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea was elaborated, the essence of which consisted in the selection of the appropriate dilution of the tinctures. Tinctures were prepared with the help of maceration or/and remaceration in laboratory conditions. The ratios of herbal raw materials (HRM) to the final tincture were close to ratios that are widely employed in the pharmaceutical industry, namely 1 to 5 and 1 to 10. The coefficients of alcohol absorption for the roots of Echinacea purpurea (size 2-5 mm), flowers of Echinacea purpurea (size 1-3 mm), herb of Monarda fistulosa (size 0.5-3 mm) were measured. They were measured as 1.2, 2.25, and 5.0 ml/g, respectively, for 70% ethanol. The studies revealed that Echinacea purpurea tinctures are a valuable source of antioxidant compounds. The antioxidant activity of these tinctures was 254.8-815.8 mg rutin-equivalents in 1 L of the tinctures or 1.12-4.43 mg rutin-equivalents in 1 g of the HRM depending on the part of the plant, particle size and extraction type. The antioxidant activity of the tinctures of the Monarda fistulosa herb was equal to 2203.6 mg eq-rutin/L and 20.3 mg eq-rutin/g for the tincture and HRM at a ratio of 1 to 9.5 and 2119.4 mg eq-rutin/L and 9.7 mg eq-rutin/g for the tincture at a ratio of 1 to 4.5.
Conclusions. The approach to the determination of the antioxidant activity of the tested tinctures was elaborated, namely the dilutions of the tinctures were established for the analytical procedure of the determination of the antioxidant activity. Our studies demonstrated that tinctures of Echinacea purpurea contained compounds with antioxidant activity. The tinctures of Monarda fistulosa herb are very rich in compounds with antioxidant properties. The laboratory technology of six tinctures was elaborated. Further studies will be directed at laboratory studies on microorganisms and animals. |
| format | Article |
| id | doaj-art-d91370118ff4455882a778782c2e35ac |
| institution | Kabale University |
| issn | 1029-4244 2415-3303 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Danylo Halytsky Lviv National Medical University |
| record_format | Article |
| series | Acta Medica Leopoliensia |
| spelling | doaj-art-d91370118ff4455882a778782c2e35ac2025-01-06T10:49:53ZengDanylo Halytsky Lviv National Medical UniversityActa Medica Leopoliensia1029-42442415-33032022-06-01281-2899810.25040/aml2022.1-2.089273METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGYOksana Yezerska0Nataliia Hudz1Lesya Kobylinska2Anna Filipska3Vira Turkina4Jan Brindza5Piotr Pawel Wieczorek6Danylo Halytsky National Medical University, Lviv, UkraineDanylo Halytsky National Medical University, Lviv, UkraineDanylo Halytsky National Medical University, Lviv, UkraineDanylo Halytsky National Medical University, Lviv, UkraineResearch Institute of Epidemiology and HygieneSlovak Agricultural University in Nitra, Nitra, Slovak RepublicUniversity of Opole, Opole, PolandAim. The aim of the study was to develop the methodical approach to determine the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea, as well as to develop their technology in laboratory conditions. Materials and Methods. The following methods were used: analysis, synthesis, systematization, and comparison for processing of published scientific data on antioxidant activity; method for measuring the particle size of raw herbal materials; maceration and remaceration methods for obtaining the tested tinctures; DPPH test for the valuation of the antioxidant activity of the developed tinctures. Results and Discussion. The methodical approach to determining the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea was elaborated, the essence of which consisted in the selection of the appropriate dilution of the tinctures. Tinctures were prepared with the help of maceration or/and remaceration in laboratory conditions. The ratios of herbal raw materials (HRM) to the final tincture were close to ratios that are widely employed in the pharmaceutical industry, namely 1 to 5 and 1 to 10. The coefficients of alcohol absorption for the roots of Echinacea purpurea (size 2-5 mm), flowers of Echinacea purpurea (size 1-3 mm), herb of Monarda fistulosa (size 0.5-3 mm) were measured. They were measured as 1.2, 2.25, and 5.0 ml/g, respectively, for 70% ethanol. The studies revealed that Echinacea purpurea tinctures are a valuable source of antioxidant compounds. The antioxidant activity of these tinctures was 254.8-815.8 mg rutin-equivalents in 1 L of the tinctures or 1.12-4.43 mg rutin-equivalents in 1 g of the HRM depending on the part of the plant, particle size and extraction type. The antioxidant activity of the tinctures of the Monarda fistulosa herb was equal to 2203.6 mg eq-rutin/L and 20.3 mg eq-rutin/g for the tincture and HRM at a ratio of 1 to 9.5 and 2119.4 mg eq-rutin/L and 9.7 mg eq-rutin/g for the tincture at a ratio of 1 to 4.5. Conclusions. The approach to the determination of the antioxidant activity of the tested tinctures was elaborated, namely the dilutions of the tinctures were established for the analytical procedure of the determination of the antioxidant activity. Our studies demonstrated that tinctures of Echinacea purpurea contained compounds with antioxidant activity. The tinctures of Monarda fistulosa herb are very rich in compounds with antioxidant properties. The laboratory technology of six tinctures was elaborated. Further studies will be directed at laboratory studies on microorganisms and animals.https://amljournal.com/index.php/journal/article/view/273echinacea purpureamonarda fistulosaextractionantioxidant activityrutin |
| spellingShingle | Oksana Yezerska Nataliia Hudz Lesya Kobylinska Anna Filipska Vira Turkina Jan Brindza Piotr Pawel Wieczorek METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGY Acta Medica Leopoliensia echinacea purpurea monarda fistulosa extraction antioxidant activity rutin |
| title | METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGY |
| title_full | METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGY |
| title_fullStr | METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGY |
| title_full_unstemmed | METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGY |
| title_short | METHODICAL APPROACH TO THE DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF THE ECHINACEA PURPUREA AND MONARDA FISTULOSA TINCTURES AS A QUALITY CRITERION OF THE DEVELOPMENT OF THEIR COMPOSITION AND LABORATORY TECHNOLOGY |
| title_sort | methodical approach to the determination of the antioxidant activity of the echinacea purpurea and monarda fistulosa tinctures as a quality criterion of the development of their composition and laboratory technology |
| topic | echinacea purpurea monarda fistulosa extraction antioxidant activity rutin |
| url | https://amljournal.com/index.php/journal/article/view/273 |
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