Expression of BMP II by human osteoblasts cultivated on dense or porous titanium
Objective: To quantified the expression of the morphogenetic bone protein II (BMP II) produced by human cells with osteoblast differentiation, after cultured over dense or porous samples of pure titanium grade II. Material and methods: The samples were produced by powder metallurgy technique. Mesen...
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| Language: | English |
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Universidade Estadual Paulista
2018-08-01
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| Series: | Brazilian Dental Science |
| Online Access: | https://ojs.ict.unesp.br/index.php/cob/article/view/1586 |
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| author | Carolina Fedel Gagliardi Luana Marotta Reis de Vasconcellos Carlos Alberto Alves Cairo Sylvia Bicalho Rabelo Renata Falchete do Prado |
| author_facet | Carolina Fedel Gagliardi Luana Marotta Reis de Vasconcellos Carlos Alberto Alves Cairo Sylvia Bicalho Rabelo Renata Falchete do Prado |
| author_sort | Carolina Fedel Gagliardi |
| collection | DOAJ |
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Objective: To quantified the expression of the morphogenetic bone protein II (BMP II) produced by human cells with osteoblast differentiation, after cultured over dense or porous samples of pure titanium grade II. Material and methods: The samples were produced by powder metallurgy technique. Mesenquimal steam cells isolated from alveolar bone of healthy donors were stimulated to differentiate, assuming an osteoblastic phenotype, by supplemented medium and plated over the samples. The experimental groups were: control, dense titanium, titanium of higher porosity and titanium of lower porosity. After 7 and 14 days, the RNA was extracted. The cDNA was prepared and the polymerase chain reaction (PCR) technique was used in real time, using the constitutive gene Beta-Actin to relative quantification. The pore morphology and interconnection were confirmed by Scanning Electron Microscopy (SEM). Results: Total porosity (obtained after apply dimensions and density formulas) and surface porosity (SEM) presented significant differences among the groups. For the group of total porosity of 33.79%, the superficial porosity was 32.5% (± 7.74%) and for the group of 41.79%, the superficial porosity was 37.4% (± 7.95%), significantly lower. The expression of BMP II was similar in all groups. Conclusion: It was concluded that the powder metallurgy has a reduced ability to standardize the porosity in the samples and that the porosity does not interfere in the cellular response of BMP II production, an important inducer of osteoblastic differentiation.
Keywords
Porous titanium; BMP II; Cell culture; PCR.
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| format | Article |
| id | doaj-art-d8d0fbdbce9a4366b6f2fd8989876f6c |
| institution | Kabale University |
| issn | 2178-6011 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Brazilian Dental Science |
| spelling | doaj-art-d8d0fbdbce9a4366b6f2fd8989876f6c2025-08-22T19:02:16ZengUniversidade Estadual PaulistaBrazilian Dental Science2178-60112018-08-0121310.14295/bds.2018.v21i3.1586Expression of BMP II by human osteoblasts cultivated on dense or porous titaniumCarolina Fedel Gagliardi0Luana Marotta Reis de Vasconcellos1Carlos Alberto Alves Cairo2Sylvia Bicalho Rabelo3Renata Falchete do Prado4São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.Division of Materials – Air and Space Institute – São José dos Campos – SP – Brazil.São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil.São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil. Objective: To quantified the expression of the morphogenetic bone protein II (BMP II) produced by human cells with osteoblast differentiation, after cultured over dense or porous samples of pure titanium grade II. Material and methods: The samples were produced by powder metallurgy technique. Mesenquimal steam cells isolated from alveolar bone of healthy donors were stimulated to differentiate, assuming an osteoblastic phenotype, by supplemented medium and plated over the samples. The experimental groups were: control, dense titanium, titanium of higher porosity and titanium of lower porosity. After 7 and 14 days, the RNA was extracted. The cDNA was prepared and the polymerase chain reaction (PCR) technique was used in real time, using the constitutive gene Beta-Actin to relative quantification. The pore morphology and interconnection were confirmed by Scanning Electron Microscopy (SEM). Results: Total porosity (obtained after apply dimensions and density formulas) and surface porosity (SEM) presented significant differences among the groups. For the group of total porosity of 33.79%, the superficial porosity was 32.5% (± 7.74%) and for the group of 41.79%, the superficial porosity was 37.4% (± 7.95%), significantly lower. The expression of BMP II was similar in all groups. Conclusion: It was concluded that the powder metallurgy has a reduced ability to standardize the porosity in the samples and that the porosity does not interfere in the cellular response of BMP II production, an important inducer of osteoblastic differentiation. Keywords Porous titanium; BMP II; Cell culture; PCR. https://ojs.ict.unesp.br/index.php/cob/article/view/1586 |
| spellingShingle | Carolina Fedel Gagliardi Luana Marotta Reis de Vasconcellos Carlos Alberto Alves Cairo Sylvia Bicalho Rabelo Renata Falchete do Prado Expression of BMP II by human osteoblasts cultivated on dense or porous titanium Brazilian Dental Science |
| title | Expression of BMP II by human osteoblasts cultivated on dense or porous titanium |
| title_full | Expression of BMP II by human osteoblasts cultivated on dense or porous titanium |
| title_fullStr | Expression of BMP II by human osteoblasts cultivated on dense or porous titanium |
| title_full_unstemmed | Expression of BMP II by human osteoblasts cultivated on dense or porous titanium |
| title_short | Expression of BMP II by human osteoblasts cultivated on dense or porous titanium |
| title_sort | expression of bmp ii by human osteoblasts cultivated on dense or porous titanium |
| url | https://ojs.ict.unesp.br/index.php/cob/article/view/1586 |
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