In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiota
Non-starch polysaccharides are major bioactive components in chestnuts, and can serve as water-soluble polysaccharides with potential prebiotic properties. This study aims to establish an in vitro digestion and fermentation model to reveal the digestive and fermentative characteristics of Non-starch...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259015752400717X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846125359494332416 |
|---|---|
| author | Fei Peng Zuoqing Yu Kui Niu Bin Du Shujun Wang Yuedong Yang |
| author_facet | Fei Peng Zuoqing Yu Kui Niu Bin Du Shujun Wang Yuedong Yang |
| author_sort | Fei Peng |
| collection | DOAJ |
| description | Non-starch polysaccharides are major bioactive components in chestnuts, and can serve as water-soluble polysaccharides with potential prebiotic properties. This study aims to establish an in vitro digestion and fermentation model to reveal the digestive and fermentative characteristics of Non-starch polysaccharides from chestnut kernels (NSPCK). The results indicated that under simulated digestion, NSPCK was partially digested in gastric juice but remained significantly undigested in saliva and intestinal juice, demonstrating considerable resilience against hydrolysis. After digestion, NSPCK still exhibited stable rough, lamellar, and porous structure and maintained strong antioxidant capacity. Animal experiments revealed positive effects of NSPCK on blood lipid level, and colon tissue of mice. Moreover, NSPCK enhanced the accumulation of short-chain fatty acids during fermentation, particularly acetic acid, propionic acid, and butyric acid. Furthermore, NSPCK intervention increased the abundance of beneficial bacteria such as Lactobacillus and Bifidobacterium, and at the same time reduced that of harmful bacteria such as Enterococcus. |
| format | Article |
| id | doaj-art-d8bcc09b6ba24daf982558af5bea3e43 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-d8bcc09b6ba24daf982558af5bea3e432024-12-13T11:01:21ZengElsevierFood Chemistry: X2590-15752024-12-0124101829In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiotaFei Peng0Zuoqing Yu1Kui Niu2Bin Du3Shujun Wang4Yuedong Yang5Hebei Key Laboratory Of Active Components and Functions In Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Qinhuangdao 066000, ChinaHebei Key Laboratory Of Active Components and Functions In Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaHebei Key Laboratory Of Active Components and Functions In Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Qinhuangdao 066000, ChinaHebei Key Laboratory Of Active Components and Functions In Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Qinhuangdao 066000, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Corresponding author.Hebei Key Laboratory Of Active Components and Functions In Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Qinhuangdao 066000, China; Corresponding author at Hebei Key Laboratory Of Active Components and Functions In Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.Non-starch polysaccharides are major bioactive components in chestnuts, and can serve as water-soluble polysaccharides with potential prebiotic properties. This study aims to establish an in vitro digestion and fermentation model to reveal the digestive and fermentative characteristics of Non-starch polysaccharides from chestnut kernels (NSPCK). The results indicated that under simulated digestion, NSPCK was partially digested in gastric juice but remained significantly undigested in saliva and intestinal juice, demonstrating considerable resilience against hydrolysis. After digestion, NSPCK still exhibited stable rough, lamellar, and porous structure and maintained strong antioxidant capacity. Animal experiments revealed positive effects of NSPCK on blood lipid level, and colon tissue of mice. Moreover, NSPCK enhanced the accumulation of short-chain fatty acids during fermentation, particularly acetic acid, propionic acid, and butyric acid. Furthermore, NSPCK intervention increased the abundance of beneficial bacteria such as Lactobacillus and Bifidobacterium, and at the same time reduced that of harmful bacteria such as Enterococcus.http://www.sciencedirect.com/science/article/pii/S259015752400717XNon-starch polysaccharidesChestnut kernelsDigestionFermentationGut microbiota |
| spellingShingle | Fei Peng Zuoqing Yu Kui Niu Bin Du Shujun Wang Yuedong Yang In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiota Food Chemistry: X Non-starch polysaccharides Chestnut kernels Digestion Fermentation Gut microbiota |
| title | In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiota |
| title_full | In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiota |
| title_fullStr | In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiota |
| title_full_unstemmed | In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiota |
| title_short | In vivo absorption, in vitro digestion, and fecal fermentation properties of non-starch polysaccharides from Chinese chestnut kernels and their effects on human gut microbiota |
| title_sort | in vivo absorption in vitro digestion and fecal fermentation properties of non starch polysaccharides from chinese chestnut kernels and their effects on human gut microbiota |
| topic | Non-starch polysaccharides Chestnut kernels Digestion Fermentation Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S259015752400717X |
| work_keys_str_mv | AT feipeng invivoabsorptioninvitrodigestionandfecalfermentationpropertiesofnonstarchpolysaccharidesfromchinesechestnutkernelsandtheireffectsonhumangutmicrobiota AT zuoqingyu invivoabsorptioninvitrodigestionandfecalfermentationpropertiesofnonstarchpolysaccharidesfromchinesechestnutkernelsandtheireffectsonhumangutmicrobiota AT kuiniu invivoabsorptioninvitrodigestionandfecalfermentationpropertiesofnonstarchpolysaccharidesfromchinesechestnutkernelsandtheireffectsonhumangutmicrobiota AT bindu invivoabsorptioninvitrodigestionandfecalfermentationpropertiesofnonstarchpolysaccharidesfromchinesechestnutkernelsandtheireffectsonhumangutmicrobiota AT shujunwang invivoabsorptioninvitrodigestionandfecalfermentationpropertiesofnonstarchpolysaccharidesfromchinesechestnutkernelsandtheireffectsonhumangutmicrobiota AT yuedongyang invivoabsorptioninvitrodigestionandfecalfermentationpropertiesofnonstarchpolysaccharidesfromchinesechestnutkernelsandtheireffectsonhumangutmicrobiota |